Fertile, advanced generation hybrids derived from crosses between Texas (Poa arachnifera Torr.) and Kentucky (Poa pratensis L.) bluegrass have been selected. The hybrids are currently being evaluated for low-input turf potential. Since they are derived from hand-harvested seed from first-generation hybrids located in field nurseries their exact genetic origin is unknown. This experiment was conducted to determine if there are still genetic relationships among the advanced generation hybrids and some of the Texas and Kentucky parents in their pedigrees. Four Inter Simple Sequence Repeat (ISSR) primer combinations resolved on 6% nondenaturing polyacrylamide gels resulted in 179 polymorphic bands that were scored to create a genetic similarity matrix and dendrogram based on Jaccard’s coefficient. The clustering of the advanced generation hybrids was generally in agreement with what would be expected based on their pedigrees and indicated it was more likely to select a fertile hybrid from an advanced generation, rather than the F1 generation.
Cite this paper
J. Goldman, "Genetic Relationships in Advanced Generation Hybrids Derived from Crosses between Texas (Poa arachnifera
) and Kentucky (Poa pratensis
) Bluegrass Using ISSR Markers," American Journal of Plant Sciences
, Vol. 4 No. 12, 2013, pp. 2440-2443. doi: 10.4236/ajps.2013.412303
 A. S. Hitchcock, “Manual of the Grasses of the United States (revised by A. Chase),” US Government Printing Office, Washington DC, 1950, p. 1051.
 J. C. Read, “Utilization of Apomictic and Dioecious Methods of Reproduction in Breeding poa spp.,” International Turfgrass Society Research Journal, No. 9, 2001, pp. 202-205.
 J. C. Read, J. A. Reinert, P. F. Colbaugh and W. E. Knoop, “Registration of ‘Reveille’ Hybrid Bluegrass,” Crop Science, Vol. 39, No. 2, 1999, p. 590.http://dx.doi.org/10.2135/cropsci1999.0011183X003900020059x
 J. J. Goldman and P. L. Sims, “Production of an Interspecific Hybrid between Texas and Argentine Bluegrass,” Plant Breeding, Vol. 124, No. 4, 2005, pp. 419-420. http://dx.doi.org/10.1111/j.1439-0523.2005.01113.x
 J. J. Goldman, “The Use of ISSR Markers to Identify Texas Bluegrass Interspecific Hybrids,” Plant Breeding, Vol. 127, No. 6, 2008, pp. 644-646.http://dx.doi.org/10.1111/j.1439-0523.2008.01526.x
 E. Zietkiewicz, A. Rafalski and D. Labuda, “Genomic Fingerprinting by Simple Sequence Repeat (SSR)-Anchored Polymerase Chain Reaction Amplification,” Genomics, Vol. 20, No. 2, 1994, pp. 176-183.http://dx.doi.org/10.1006/geno.1994.1151
 S. O. Rogers and A. J. Bendich, “Extraction of DNA from Milligram Amounts of Fresh, Herbarium and Mummified Plant Tissues,” Plant Molecular Biology, Vol. 5, No. 2, 1985, pp. 69-76.
 J. R. Brody and S. E. Kern, “Sodium Boric Acid: A Tris-Free, Cooler Conductive Medium for DNA Electrophoresis,” BioTechniques, Vol. 36, No. 2, 2004, pp. 214-216.
 P. Jaccard, “Nouvelles Recherches sur la Distribution Florale,” Bulletin de la Société Vaudoise des Sciences Naturelles, Vol. 44, No. 163, 1908, pp. 223-270.
 F. J. Rohlf, “NTSYS-PC: Numerical Taxonomy and Multivariate Analysis System, Version 2.02i,” Department of Ecology and Evolution, State University of New York, Setauket, 1998.