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 AS  Vol.4 No.12 A , December 2013
The role of green manure nitrogen use by corn and sugarcane crops in Brazil
Abstract: Three assays were developed from April 3, 1995 to October 10, 2005. The work with corn was conducted in a greenhouse, using velvet bean (Mucuna aterrima) and sunn hemp (Crotalaria juncea) as green manure with 15N labeling of either shoots or roots, in two soils with contrasting textural classes. The mineralization of N from legume plants incorporated into the two soils was investigated too. This work included two green manures: velvet bean and sunn hemp, and the common bean (Phaseolus vulgaris) residues. Nitrogen from the velvet bean accounted for a greater proportion of the soil inorganic N; shoots were responsible for most of N accumulated. Common bean residues caused immobilization of inorganic N. The leguminous species added were intensively and promptly mineralized, preserving the soil native nitrogen. One hundred days after emergence of the corn, velvet bean provided higher accumulation of nitrogen in the soil, higher absorption by corn plants and accumulation in the aerial part. The green manure decomposition was more intense in the medium textured soil. In this soil, highest nitrogen losses were also observed. The sugarcane (Saccharum spp.) was cultivated for five years in the field and was harvested three times; 15N recovery was evaluated in the first two harvests. The combination of inorganic fertilizer and green manure resulted in higher sugarcane yields than either N source applied separately; however, in the second cutting the yields were higher where sunn hemp was used than in plots with ammonium sulfate. The recovery of N by the first two consecutive harvests accounted for 19% to 21% of the N applied as sunn hemp and 46% to 49% of the N applied as ammonium sulfate. Very little inorganic N was present in the 0-40 cm soil layer with both N sources.  
Cite this paper: Ambrosano, E. , Cantarella, H. , Bovi Ambrosano, G. , Ferreira Dias, F. , Rossi, F. , Ocheuze Trivelin, P. and Muraoka, T. (2013) The role of green manure nitrogen use by corn and sugarcane crops in Brazil. Agricultural Sciences, 4, 89-108. doi: 10.4236/as.2013.412A008.
References

[1]   Pankhurst, C.E., Stirling, G.R., Magarey, R.C., Blair, B.C., Holt, J.A., Bell, M.J. and Garside, A.L. (2005) Quantification of the effects of rotation breaks on soil biological properties and their impact on yield decline in sugarcane. Soil Biology and Biochemistry, 37, 1121-1130.
http://dx.doi.org/10.1016/j.soilbio.2004.11.011

[2]   Shoko, M.D. and Zhou, M. (2009) Nematode diversity in a soybean-sugarcane production system in a semi-arid region of Zimbabwe. Journal of Entomology and Nematology, 1, 25-28.

[3]   Garside, A.L., Berthelsen, J.E., Richards, C.L. and Toovey, L.M. (1996) Fallow legumes on the wet tropical coast: Some species and management options. Proceedings of the Australian Society of Sugar Cane Technologists, 18, 202-208.

[4]   Shoko, M.D. and Tawira, F. (2007) Benefits of soyabeans as a breakcrop in sugarcane production systems in the South Eastern Lowveld of Zimbabwe. Sugar Journal, 70, 18-22.

[5]   Mc Mahon, G.G., Williams, R.C. and Mc Guire, P.J. (1989) The effects of weed competition on ratoon sugar cane yield. Proceedings of the Australian Society of Sugar Cane Technologists, 10, 88-92.

[6]   Cheruiyot, E.K., Mumera, L.M., Nakhone, L.N. and Mwonga, S.M. (2003) Effect of legumemanaged fallow on weeds and soil nitrogen in following maize (Zea mays L.) and wheat (Triticum aestivum L.) crops in the Rift Valley highlands of Kenya. Australian Journal of Experimental Agriculture, 43, 597-604.
http://dx.doi.org/10.1071/EA02033

[7]   Jannink, J.L., Liebman, M. and Merrick, L.C. (1996) Biomass production and nitrogen accumulation in pea, oat, and vetch green manure mixtures. Agronomy Journal, 88, 231-240.
http://dx.doi.org/10.2134/agronj1996.00021962008800020019x

[8]   Ambrosano, E.J., Trivelin, P.C.O., Cantarella, H., Ambrosano, G.M.B., Schammass, E.A., Guirado, N., Rossi, F., Mendes, P.C.D. and Muraoka, T. (2005) Utilization of nitrogen from green manure and mineral fertilizer by sugarcane. Scientia Agricola, 62, 534-542.
http://dx.doi.org/10.1590/S0103-90162005000600004

[9]   IBGE (2010) Systematic survey of agricultural production.
http://www.sidra.ibge.gov.br/bda/default.asp? t=5&z=t&o= 1 & u 1 = 1 & u 2 = 1 & u 3 = 1 & u 4 = 1 & u 5 = 1 & u 6 = 1 & u 7 = 1&u8=1&u9=3&u10=1&u11=26674&u12=1&u13=1&u14=1

[10]   Ambrosano, E.J., Azcón, R., Ambrosano, G.M.B., Cantarella, H., Guirado, N., Muraoka, T., Rossi, F., Schammass, E.A., Trivelin, P.C.O., Ungaro, M.R. and Teramoto, S.J.R. (2010) Crop rotation biomass and arbuscular mycorrhizal fungi effects on sugarcane yield. Scientia Agricola, 67, 692-701.

[11]   Dinardo-Miranda, L.L. and Fracasso, J.V. (2009) Spatial distribution of plantparasitic nematodes in sugarcane fields. Scientia Agricola, 66, 188-194.
http://dx.doi.org/10.1590/S0103-90162009000200007

[12]   Ambrosano, E.J., Ambrosano, G.M.B., Azcón, R., Cantarella, H., Dias, F.L.F., Muraoka, T., Trivelin, P.C.O., Rossi, F., Schammass, E.A. and Sachs R.C.C. (2011) Productivity of sugarcane after previous legumes crop. Bragantia, 70, 1-9. (in Portuguese, with abstract in English)

[13]   Muraoka, T., Ambrosano, E.J., Zapata, F., Bortoletto, N., Martins, A.L.M., Trivelin, P.C.O., Boaretto, A.E. and Scivittaro, W.B. (2002) Efficiency of green manure (Crotalaria and Mucuna) and urea as nitrogen source for rice crop. Terra, 20, 17-23. (in Portuguese, with abstract in English)

[14]   Ambrosano, E.J., Trivelin, P.C.O., Cantarella, H., Ambrosano, G.M.B., Schammass, E.A., Muraoka, T., Guirado, N. and Rossi, F. (2009) Nitrogen supply to maize from sunn hemp and velvet bean green manures. Scientia Agricola, 66, 386-394.
http://dx.doi.org/10.1590/S0103-90162009000300014

[15]   Ambrosano, E.J., Trivelin, P.C.O., Cantarella, H., Ambrosano, G.M.B., Schammass, E.A., Muraoka, T. and Rossi, F. (2011) 15N-labeled nitrogen from green manure and ammonium sulfate utilization by the sugarcane ratoon. Scientia Agricola, 68, 361-368.
http://dx.doi.org/10.1590/S0103-90162011000300014

[16]   Albuquerque, G.A.C., Araújo Filho, J.T. and Marinho, M.L. (1980) Green manure and its economic importance. Buletim IAA/PLANALSUCAR, 1, 1-10. (in Portuguese)

[17]   Mascarenhas, H.A.A., Nogueira, S.S.S., Tanaka, R.T., Martins, Q.A.C. and Carmello, Q.A.C. (1998) Effect of productivity of crop rotation and summer and sunn henp in the winter. Scientia Agricola, 55, 534-537. (in Portuguese, with abstract in English)
http://dx.doi.org/10.1590/S0103-90161998000300024

[18]   Kanthack, R.A.D., Mascarenhas, H.A.A., Castro, O.M. and Tanaka, R.T. (1991) Nitrogen applied as side dressing in corn after white lupine. Brazilian Agricultural Research, 26, 99-104. (in Portuguese, with abstract in English)

[19]   Ambrosano, E.J, Trivelin, P.C.O., Cantarella, H., Ambrosano, G.M.B. and Muraoka, T. (2003) Nitrogen mineralizetion in soils amended with sunnhemp, velvet bean and common bean residues. Scientia Agricola, 60, 133-137.
http://dx.doi.org/10.1590/S0103-90162003000100020

[20]   Ambrosano, E.J., Trivelin, P.C.O. and Muraoka, T. (1997) A technique developed for labeling the green manures (sunnhemp and velvet bean) with 15N for nitrogen dynamic studies. Bragantia, 56, 219-224. (in Portuguese, with abstract in English)

[21]   Buresh, R.J., Austin, E.R. and Craswell, E.T. (1982) Analytical methods in 15N research. Fertilizer Research, 3, 37-62. http://dx.doi.org/10.1007/BF01063408

[22]   Rittenberg, D. (1946) The preparation of gas sample for mass-spectrometric analysis. In Wilson, D.W., Ed., Preparation and Measure of Isotopic Tracers, Ann Arbor, Michigan, 31.

[23]   International Atomic Energy Agency. (1976) Tracer manual on crops and soils. IAEA, Viena, 277. (Tecnical Reports Series, 171)

[24]   Bremner, J.M. (1996) Nitrogen-total. In: Sparks, D.L., Ed., Methods of Soil Analysis, Part 3, Soil Science Society of America, Madison, 1085-1121.

[25]   Proksch, G. (1969) Routine analysis of 15N in plant material by mass-spectrometry. Plant and Soil, 31, 380-384.
http://dx.doi.org/10.1007/BF01373583

[26]   Trivelin, P.C.O., Salati, E. and Matsui, E. (1973) Preparation of samples for 15N analysis by mass spectrometry. USP/CENA, Piracicaba, 41. (Technical Bulletin, 2, in Portuguese, with abstract in English)

[27]   Ambrosano, E.J., Trivelin, P.C.O., Cantarella, H., Rossetto, R., Muraoka, T., Bendassolli, J.A., Ambrosano, G.M.B., Tamiso, L.G., Vieira, F.C. and Prada Neto, I. (2003) Nitrogen-15 labeling of Crotalaria juncea green manure. Scientia Agricola, 60, 181-184.

[28]   Tanimoto, T. (1964) The press method of cane analysis. Hawaiian Planter’s Record, 57, 133-150.

[29]   Trivelin, P.C.O., Lara Cabezas, W.A.R., Victoria, R.L. and Reichardt, K. (1994) Evaluation of a 15N plot design for estimating plant recovery of fertilizer nitrogen applied to sugar cane. Scientia Agricola, 51, 226-234.
http://dx.doi.org/10.1590/S0103-90161994000200005

[30]   Tanaka, R.T., Mascarenhas, H.A.A., Dias, O.S., Campidelli, C. and Bulisani, E.A. (1992) Soybean cultivation after incorporation of green manure and organic compost. Brazilian Agricultural Research, 27, 1477-1483. (in Portuguese, with abstract in English)

[31]   Jans-Hammermeister, D.C., McGill, W.B. and Jensen, T.L. (1994) Nitrogen accumulations and relative rates of mineralization in two soils following legume green manuring. Canadian Journal of Soil Science, 74, 23-28.
http://dx.doi.org/10.4141/cjss94-003

[32]   Calvache Ulloa, A.M., Libardi, P.L. and Reichardt, K. (1982) Utilization of nitrogen fertilizer by two corn hybrids. Cargill Foundation, Campinas, 66.

[33]   Fried, D.M. and Broeshart, H. (1974) Priming effect of nitrogen fertilizer on soil nitrogen. Proceedings of the Soil Science Society of America, 38, 858-864.
http://dx.doi.org/10.2136/sssaj1974.03615995003800050046x

[34]   Jenkinson, D.S., Fox, R.H. and Rayner, J.H. (1985) Interaction between fertilizer nitrogen and soil nitrogen—The so-called “Priming” effect. Journal of Soil Science, 36, 425-444.
http://dx.doi.org/10.1111/j.1365-2389.1985.tb00348.x

[35]   Hart, P.B.S., Rayner, J.H. and Jenkinson, D.S. (1986) Influence of pool substitution on the interpretation of fertilizer experiments with 15N. Journal of Soil Science, 37, 389-403.
http://dx.doi.org/10.1111/j.1365-2389.1986.tb00372.x

[36]   Rao, A.C.S., Smith, J.L., Parr, J.F. and Papendick, R.J. (1992) Considerations in estimating nitrogen recovery efficiency by the difference and isotopic dilution methods. Fertilizer Research, 33, 209-217.
http://dx.doi.org/10.1007/BF01050876

[37]   Ambrosano, E.J., Ambrosano, G.M.B., Corsi, M., Cantarella, H., Trivelin, P.C.O. and Muraoka, T. (1996) Utilization of nitrogen by ammonium sulfate (15N) at grasscolonião at successive cuts in different seasons and fertilization. Brazilian Journal of Soil Science, 20, 277-282. (in Portuguese, with abstract in English)

[38]   Stevenson, F.J. (1982) Nitrogen in agricultural soils. In: Organic Forms of Soil Nitrogem, ASA, Madison, 67-122.

[39]   Ladd, J.N., Amato, M., Jackson, R.B. and Butler, J.H.A. (1983) Utilization by wheat crops of nitrogen from legume residues decomposing in soils in the field. Soil Biology and Biochemistry, 15, 231-238.
http://dx.doi.org/10.1016/0038-0717(83)90064-0

[40]   Stevenson, F.J. (1994) Humus chemistry: Genesis, composition, reactions. 2nd Edition, John Wiley, New York, 95-97.

[41]   Bayer, C., Mielniczuk, J., Martin-Neto, L. and Errnani, P.R. (2004) Stocks and humification degree of organic matter fractions as affected by no-tillage on a subtropical soil. Plant and Soil, 238, 133-140.

[42]   Bertoncini, E.I., D’Orazio, V., Senesi, N. and Mattiazzo, M.E. (2005) Fluorescence analysis of humic and fulvic acids from two Brazilian oxisols as affected by biosolid amendment. Analytical and Bioanalytical Chemistry, 381, 1281-1288. http://dx.doi.org/10.1007/s00216-005-3054-2

[43]   Ladd, J.N., Oades, J.M. and Amato, M. (1981) Microbial biomass formed from 14C, 15N-labeled plant material decomposing in soils in the field. Soil Biology and Biochemistry, 13, 119-126.
http://dx.doi.org/10.1016/0038-0717(81)90007-9

[44]   Bartholomew, W.V. (1965) Mineralization and immobilization of nitrogem in the decomposition of plant and animal residues. In: Bartholomew, W.V. and Clark, F.E., Eds., Soil Nitrogen, American Society of Agronomy, Madison, 287-306. (American Society of Agronomy Monograph, 10)

[45]   Flores, L.M. (1986) Quantitative evaluation of the efficiency of use of two nitrogen sources, CO(15NH2)2 e (15NH4)2SO4 by maize crops (Zea mays L.). Master’s Thesis, USP/ESALQ, Piracicaba, 103.

[46]   Villas Bôas, R.L. (1990) Alternatives to increase the recovery of urea nitrogen by corn (Zea mays L). Master’s Thesis, USP/ESALQ, Piracicaba, 78.

[47]   Ng Kee Kwong, K.F. and Deville, J. (1994) Application of 15N-labelled urea to sugar cane through a drip-irrigation system in Mauritius. Fertilizer Research, 39, 223-228. http://dx.doi.org/10.1007/BF00750250

[48]   Farquhar, G.D., Wetselaar, R. and Firth, P.M. (1979) Ammonia volatilization from senescing leaves of maize. Science, 203, 1257-1258.
http://dx.doi.org/10.1126/science.203.4386.1257

[49]   Stutte, C.A., Weiland, R.T. and Blem, A.R. (1979) Gaseous nitrogen loss from soybean foliage. Agronomy Journal, 71, 95-97.

[50]   Silva, P.R.F. and Stutte, C.A. (1981) Nitrogem loss in conjunction with transpiration from rice leaves as influenced by growth stage, leaf position and N supply. Agronomy Journal, 73, 38-48.
http://dx.doi.org/10.2134/agronj1981.00021962007300010009x

[51]   Allison, F.E. (1966) The fate of nitrogen applied to soils. Advances in Agronomy, 18, 219-258.
http://dx.doi.org/10.1016/S0065-2113(08)60651-3

[52]   Azam, F., Malik, K.A. and Sajjad, M.I. (1985) Transformations in soil and availability to plants of 15N applied as inorganic fertilizer and legume residues. Plant and Soil, 86, 3-13. http://dx.doi.org/10.1007/BF02185020

[53]   Silva, E.C., Muraoka, T., Buzetti, S., Guimarã es, G.L., Trivelin, P.C.O. and Veloso, M.E.C. (2006) Utilization of residual nitrogen (15N) by urea cover crops by corn. Brazilian Journal of Soil Science, 30, 965-974. (in Portuguese, with abstract in English)

[54]   Ladd, J.N., Oades, J.M. and Amato, M. (1981) Distribution and recovery of nitrogem from legume residues decomposing in soils sown to wheat in the field. Soil Biology and Biochemistry, 13, 251-256.
http://dx.doi.org/10.1016/0038-0717(81)90058-4

[55]   Harris, G.H. and Hesterman, O.B. (1990) Quantifying the nitrogen contribution from alfafa to soil and two succeeding crops using nitrogen-15. Agronomy Journal, 82, 129-134. http://dx.doi.org/10.2134/agronj1990.00021962008200010028x

[56]   Sakai, R.H., Ambrosano, E.J., Guirado, N., Rossi, F., Mendes, P.C.D., Cantarella, H., Arevalo, R.A. and Ambrosano, G.M.B. (2007) Agronomic evaluation of four species of mucuna used as green manure in agro ecological systems. Brazilian Journal of Agroecology, 2, 910-913. (in Portuguese, with abstract in English)

[57]   Franchini, J.C., Meda, A.R., Cassiolato, M.E., Miyazawa, M. and Pavan, M.A. (2001) Plant residue extracts potential for lime mobility in the soil using a biological method. Scientia Agricola, 58, 357-360.
http://dx.doi.org/10.1590/S0103-90162001000200020

[58]   Humbert, R.P. (1968) Nutrition of sugar cane. In: The Growing of Sugar Cane, Elsevier, Amsterdan, 133-309.

[59]   Silva, L.C.F. and Casagrande, J.C. (1983) Mineral Nutrition of sugarcane (macronutrientes). In: Orlando Filho, J. Ed., Nutrition and Fertilization of Sugarcane in Brazil. IAA Planalsucar, Piracicaba, 77-99. (in Portuguese)

[60]   Mascarenhas, H.A.A., Tanaka, R.T., Costa, A.A., Rosa, F.V. and Costa, V.F. (1994) Residual effects of legumes on the physical and economic yield of sugarcane. Instituto Agronômico, Campinas. (in Portuguese)

[61]   Rochester, I. and Peoples, M. (2005) Growing vetches (Vicia Villosa Roth) in irrigated cotton systems: Inputs of fixed N, N fertilizer savings and cotton productivity. Plant and Soil, 271, 251-264.
http://dx.doi.org/10.1007/s11104-004-2621-1

[62]   Silveira, J.A.G. and Crocomo, O.J. (1990) Nitrogen assimilation in sugarcane plants as affected by high levels of N and vinasse in the soil. Brazilian Journal of Plant Physiology, 2, 7-15.

[63]   Freney, J.R., Denmead, O.T., Wood, A.W., Saffigna, P.G., Chapman, L.S., Ham, G.J., Hurney, A.P. and Stewart, R.L. (1992) Factors controlling ammonia loss from trash covered sugarcane fields fertilized with urea. Fertilizer Resarch, 31, 341-349.
http://dx.doi.org/10.1007/BF01051285

[64]   Gava, G.J.C., Trivelin, P.C.O., Vitti, A.C. and Oliveira, M.W. (2003) Recovery of nitrogen (15N) from urea and cane trash by sugar cane ratoon (Saccharum spp.). Brazilian Agricultural Research, 27, 621-630. (in Portuguese, with abstract in English)

[65]   Trivelin, P.C.O., Rodrigues, J.C.S. and Victoria, R.L. (1996) Utilization by early harvest sugar cane ratoon of the nitrogen from 15N-aqua ammonia and 15N-urea applied to the soil as vinasse N-complement. Brazilian Agricultural Research, 31, 89-99. (in Portuguese, with abstract in English).

[66]   Cantarella, H., Trivelin, P.C.O. and Vitti, A.C. (2007) Nitrogen and sulfur in the sugar cane. In: Yamada, T., Abdalla, S.R.S. and Vitti, G.C., Eds., Nitrogen and Sulfur in the Brazilian Agriculture, International Plant Nutrition Institute, Piracicaba, 355-412.

[67]   Perin, A., Santos, R.H.S., Urquiaga, S., Guerra, J.G.M. and Cecon, P.R. (2006) Sunn hemp and millet as green manure for tropical maize production. Scientia Agricola, 63, 453-459.
http://dx.doi.org/10.1590/S0103-90162006000500006

[68]   Silva, E.C., Muraoka Buzetti, S., Veloso, M.E.C. and Trivelin, P.C.O. (2006) Utilization of nitrogen (15N) from sun hemp and millet by corn crop under no-tillage in Red Latosol of Cerrado. Rural Science, 36, 739-746. (in Portuguese, with abstract in English)

[69]   Seo, J., Meisinger, J.J. and Lee, H. (2006) Recovery of nitrogen-15-labeled hairy veth and fertilizer applied to corn. Agronomy Journal, 98, 245-254.
http://dx.doi.org/10.2134/agronj2005.0013

[70]   Boaretto, A.E., Spolidoria, E.S., Freitas, J.G., Trivelin, P.C.O., Muraoka, T. and Cantarella, H. (2004) Fate of 15N-urea applied to wheat-soybean succession crop. Bragantia, 63, 265-274. (in Portuguese, with abstract in English)
http://dx.doi.org/10.1590/S0006-87052004000200011

[71]   Basanta, M.V., Dourado Neto, D., Reichardt, K., Bacchi, O.O.S., Oliveira, J.C.M., Trivelin, P.C.O., Timm, L.C., Tominaga, T.T., Correchel, V., Cassaro, F.A.M., Pires, L.F. and Macedo, J.R. (2003) Management effects on nitrogen recovery in a sugarcane crop grown in Brazil. Geoderma, 116, 235-248.
http://dx.doi.org/10.1016/S0016-7061(03)00103-4

[72]   Asagi, N. and Ueno, H. (2009) Nitrogen dynamics in paddy soil applied with various 15N-labelled green manures. Plant and Soil, 322, 251-262.
http://dx.doi.org/10.1007/s11104-009-9913-4

[73]   Ghiberto, P.J.L., Libardi, P.L., Brito, A.S. and Trivelin, P.C.O. (2009) Leaching of nutrients from a sugarcane crop growing on an Ultisol in Brazil. Agricultural Water Management, 96, 1443-1448.
http://dx.doi.org/10.1016/j.agwat.2009.04.020

 
 
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