MSCE  Vol.1 No.7 , December 2013
Bamboo Fibers Elaborating Cellulose Hydrogel Films for Medical Applications
ABSTRACT

Bamboo fibers were used as source to prepare cellulose hydrogel films for cell cultivation scaffold. The preparation of cellulose solutions was carried out by three different dissolving methods with NaOH-based and NaOH/urea aqueous solutions and DMAc/LiCl solution. Several hydrogel films were elaborated and their properties were compared to evaluate the effect of the dissolving method. It was found that tensile strength of the resultant hydrogel films increased from 21 to 66 N/mm2 when DMAc/LiCl was used instead of the NaOH/urea solution. The same tendency was observed in the obtained elongation values. Moreover, a remarkable difference in fibroblast cell cultivation was observed in higher cell density, when DMAc/LiCl method was used. The obtained results with DMAc/LiCl also were seen to be higher than the results for PS dish used as control. However, low cytocompatibility was observed when NaOH and NaOH/urea methods were used. The obtained results showed that hydrogel films elaborated with cellulose solution prepared with DMAc/LiCl method exhibited good cytocompatibility for the cell cultivation scaffold.


Cite this paper
Tovar-Carrillo, K. , Tagaya, M. and Kobayashi, T. (2013) Bamboo Fibers Elaborating Cellulose Hydrogel Films for Medical Applications. Journal of Materials Science and Chemical Engineering, 1, 7-12. doi: 10.4236/msce.2013.17002.
References
[1]   F. A. Muller, L. Muller, I. Hofman, P, Greil, M. M. Wenzel and R. Staudenmaier, “Cellulose Based Scaffold Materials for Cartilage Tissue Engineering,” Biomaterials, Vol. 27, No. 21, 2006, pp. 3955-3963.
http://dx.doi.org/10.1016/j.biomaterials.2006.02.031

[2]   H. Hoenich, “Cellulose for Medical Applications: Past, Present, and Future,” Bioresources, Vol. 2, No. 2, 2006, pp. 270-280.

[3]   K. L. Tovar-Carrillo, M. Tagaya and T. Kobayashi, “Fibroblast Compatibility on Scaffold Hydrogels Prepared from Agave Tequilana Weber Bagasse for Tissue Regeneration,” Industrial & Engineering Chemistry Research, Vol. 52, No. 33, 2013, pp. 11607-11613.
http://dx.doi.org/10.1021/ie401793w

[4]   W. Deoliveira and W. G. Glasser, “Hydrogels from Polysaccharides. 1. Cellulose Beads for Chromatographic,” Journal of Applied Polymer Science, Vol. 60, No. 1, 1996, pp. 63-73.
http://dx.doi.org/10.1002/(SICI)1097-4628(19960404)60:1<63::AID-APP8>3.0.CO;2-T

[5]   H. Saito, A. Sakurai, M. Sakakibara and H. Saga, “Preparation and Properties of Transparent Cellulose Hydrogels,” Journal of Applied Polymer Science, Vol. 90, No. 11, 2002, pp. 3020-3025.
http://dx.doi.org/10.1002/app.13015

[6]   S. Zhang, F. X. Li and J. Y, Yu, “Preparation of Cellulose/Chitin Blend Bio-Fibers via Direct Dissolution,” Cellulose Chemistry and Technology, Vol. 43, No. 9-10, 2009, pp. 393-398.

[7]   C. F. Liu, R. C. Sun, A. P. Zhang and J. L. Ren, “Preparation of Sugarcane Bagasse Cellulosic Phthalate Using an Ionic Liquid as Reaction Medium,” Carbohydrate Polymers, Vol. 68, No. 1, 2007, pp. 17-25.
http://dx.doi.org/10.1016/j.carbpol.2006.07.002

[8]   M. Abe, Y. Fukaya and H. Ohno, “Fast and Factible Dissolution of Cellulose with Tetrabutylphosphonium Hydroxide Containing 40 wt% Water,” Chemical Communications, Vol. 48, No. 12, 2012, pp. 1808-1810.
http://dx.doi.org/10.1039/c2cc16203b

[9]   L. Yan and Z. Gao, “Dissolving of Cellulose in PEG/ NaOH Aqueous Solution,” Cellulose, Vol. 15, No. 6, 2008, pp. 789-796.
http://dx.doi.org/10.1007/s10570-008-9233-5

[10]   J, Vitz, T. Erdmenger, C. Haensch and U. S. Schubert, “Extended Dissolution Studies of Cellulose in Imidazolium Based Ionic Liquids,” Green Chemistry, Vol. 11, No. 3, 2009, pp. 417-424.
http://dx.doi.org/10.1039/b818061j

[11]   E. Sjoholm, K. Gustafsson, B. Eriksson, W. Brown and A. Colmjo, “Aggregation of Cellulose in Lithium Chloride/ N,N-dimethylacetamide,” Carbohydrate Polymers, Vol. 41, No. 2, 2000, pp. 153-161.
http://dx.doi.org/10.1016/S0144-8617(99)00080-6

[12]   E. Sjoholm, K, Gustafsson, B. Pettersson and A. Colmsjo, “Characterization of the Cellulosic Residues from Lithium Chloride/N,N-dimethylacetamide Dissolution of Softwood Kraft Pulp,” Carbohydrate Polymers, Vol. 32, No. 1, 1997, pp. 57-63.
http://dx.doi.org/10.1016/S0144-8617(96)00129-4

[13]   A. Ostlund, D. Lundberg, L. Nordstierna, K. Holmberg and M. Nyden, “Dissolution and Gelation of Cellulose in TBAF/DMSO Solutions: The Roles of Fluoride Ions and Water,” Biomacromolecules, Vol. 10, No. 9, 2009, pp. 2401-2407. http://dx.doi.org/10.1021/bm900667q

[14]   B. Lindman, G. Karlstrom and L. Stigsson, “On the Mechanism of Dissolution of Cellulose,” Journal of Molecular Liquids, Vol. 156, No. 1, 2010, pp. 76-81.
http://dx.doi.org/10.1016/j.molliq.2010.04.016

[15]   A. M. Striegel, “Theory and Applications of DMAc/LiCl in the Analysis of Polysaccharides,” Carbohydrate Polymers, Vol. 34, No. 4, 1997, pp. 267-274.
http://dx.doi.org/10.1016/S0144-8617(97)00101-X

[16]   S. Zhang, F. X. Li, J. Y. Yu and Y. L. Hsieh, “Dissolution Behavior and Solubility If Cellulose in NaOH Complex Solution,” Carbohydrate Polymers, Vol. 81, No. 3, 2010, 668-674.
http://dx.doi.org/10.1016/j.carbpol.2010.03.029

[17]   A. Isogai and R. H. Atalla, “Dissolution of Cellulose in Aqueous NaOH Solutions,” Cellulose, Vol. 5, No. 4, 1995, pp. 309-319.
http://dx.doi.org/10.1023/A:1009272632367

[18]   S. Zhang, F. X. Li, J. Y. Yu and G. L. Xia, “Dissolved State and Viscosity Properties of Cellulose in a NaOH Complex Solvent,” Cellulose Chemistry and Technology, Vol. 43, No. 9-10, 2009, pp. 241-249.

[19]   H. Jin, C. Zha and L. Gu, “Direct Dissolution of Cellulose in NaOH/Thiourea/Urea Aqueous Solution,” Carbohydrate Research, Vol. 342, No. 6, 2007, pp. 851-858.
http://dx.doi.org/10.1016/j.carres.2006.12.023

[20]   J. Behin, F. Mikanikl and Z. Fadael, “Dissolving Pulp (Alpha-Cellulose) from Corn Stalk by Kraft Process,” Iranian Journal of Chemistry Engineering, Vol. 5, No. 3, 2008, pp. 14-28.

[21]   J. Zhou and L. Zhang, “Solubility of Cellulose in NaOH/ Urea Aqueous Solution,” Polymer Journal, Vol. 32, No. 10, 2000, pp. 866-870.

[22]   L. A. Ramos, D. L. Morgado, O. A. El Seoud, V. C. da Silva and E. Frollini, “Acetylation of Cellulose in LiClN,N-Dimethylacetamide: First Report on the Correlation between the Reaction Effiency and the Aggregation Number of Dissolved Cellulose,” Cellulose, Vol. 18, No. 2, 2011, pp. 385-392.
http://dx.doi.org/10.1007/s10570-011-9496-0

[23]   D. Ishii, D. Tatsumi and T. Matsumoto, “Effect of Solvent Exchange on the Supramolecular Structure, the Molecular Mobility and the Dissolution Behaviour of Cellulose in LiCl/DMAc,” Carbohydrate Research, Vol. 343, No. 5, 2008, pp. 919-928.
http://dx.doi.org/10.1016/j.carres.2008.01.035

[24]   N. G. Wanhg, J. Kim, Y. Chen, S. R. Yun and S. K. Lee, “Electro-Active-Paper Actuator Made with LiCl/Cellulose Films: Effect of LiCl content,” Macromolecular Research, Vol. 14, No. 6, 2006, pp. 624-629.
http://dx.doi.org/10.1007/BF03218734

[25]   H. Qi, Q. Yang, L. Zhang, T. Liebert and T. Heinze, “The Dissolution of Cellulose in NaOH-based Aqueous System by Two-Step Process,” Cellulose, Vol. 18, No. 2, 2011, pp. 237-245.
http://dx.doi.org/10.1007/s10570-010-9477-8

[26]   K. Mequanint, A. Patel and D. Bezuidenhout, “Synthesis, Swelling Behaviour, and Biocompatibility of Novel Physically Cross-Linked Polyurethane-Block-POLY (Glycerol Methacrylate) Hydrogels,” Biomacromolecules, Vol. 7, No. 3, 2006, pp. 883-891.
http://dx.doi.org/10.1021/bm0507047

[27]   T. R. Dawsey and C. L. McCormick, “The Lithium Chloride/Dimethylacetamide Solvent for Cellulose: A Literature Review,” Journal of Macromolecular Science, Part C: Polymers Reviews, Vol. 30, No. 3-4, 1990, pp. 405440. http://dx.doi.org/10.1080/07366579008050914

[28]   A. K. Salem, S. J. Tendler, C. J. Roberts, “Interactions of 3T3 Fibroblasts and Endothelial Cells with Defined Pore Features,” Journal of Biomedical Materials Research, Vol. 61, No. 2, 2002, pp. 212-217.
http://dx.doi.org/10.1002/jbm.10195

 
 
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