The complete coding sequence ofRaphanus sativusWUS gene was obtained by usingArabidopsis thalianagene se-quence (NM_127349) as a probe in silico cloning. Then the hydrophilicity, secondary structure and advanced structure of WUS protein inRaphanus sativusare analyzed by using bioinformatics methods. The results show that the cDNA was 1298 bp, with no intron, contains an open reading frame of 936bp, encoding 312aa protein. The protein coded byRaphanus sativusgene showed 74% similarity toArabidopsis thaliana.
 I. Baurle and T. Laux, “Regulation of WUSCHEL Transcription in the Stem Cell Niche of the Arabidopsis Shoot Meristem,” Plant Cell, Vol. 17, 2005, pp. 2271-2280. http://dx.doi.org/10.1105/tpc.105.032623
 G. Sena, X. Wang, H. Y. Liu, H. Hofhuis and K. D. Birnbaum, “Organ Regeneration Does Not Require a Functional Stem Cell Niche in Plants,” Nature, Vol. 457, 2009, pp. 1150-1153. http://dx.doi.org/10.1038/nature07597
 T. Kondo, S. Sawa A. Kinoshita, S. Mizuno, T. Kakimoto, H. Fukuda and Y. Sakagami, “A Plant Peptide Encoded by CLV3 Identified by in Situ MALDI-TOF MS Analysis,” Science, Vol. 313, 2006, pp. 845-848. http://dx.doi.org/10.1126/science.1128439
 U. Brand, M. Grünewald, M. Hobe and R. Simon, “Regulation of CLV3 Expression by Two Homeobox Genes in Arabidopsis,” Plant Physiology, Vol. 129, 2002, pp. 565- 575. http://dx.doi.org/10.1104/pp.001867
 H. Li, G. Y. Zhou, H. Y. Zhang, L. Lin and J. Liu, “In Silico Cloning and Bioinformatic Analysis of PEPCK Gene in Fusarium oxysporum,” African Journal of Biotechnology, Vol. 9, 2010, pp. 1864-1870.