Chromosome segmentation is the most important step in the automatic chromosome analysis system. Since in almost every metaphase image partial touching and overlapping of chromosomes are a common phenomenon, how to separate these chromosomes correctly is a difficult yet vital problem. A number of attempts have been made to deal with this problem. This paper is focused on these attempts. Some algorithms are investigated. The principle and the realization of these algorithms are analyzed. Results of these algorithms are compared and discussed.
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