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 AiM  Vol.3 No.8 , December 2013
Rapid Detection Method for Enteroaggregative Escherichia coli Using Simple Clump Formation and Aggregative Assay
Abstract: Enteroaggregative Escherichia coli (EAggEC) strains cause the persistent diarrhea in infants and compromised hosts in developing countries. These strains are currently defined as E. coli that adheres to HEp-2 cells in an aggregative adherence (AA) pattern. In this study, we compared 4 different rapid methods for the detection of EAggEC using a PCR assay, clump formation test, glass slide adherence assay, and the HEp-2 cell adherence assay. Out of 683 E. coli strains isolated from diarrheal stool samples, we detected 17 aggR and/or clump-positive strains, and identified 2 aggR-positive, clump-negative strains and 2 aggR-negative, clump-positive strains. All the aggR positive and clump positive strains also showed positive results in glass slide adherence and HEp-2 cell adherence assays. From all these results, we suggest the following procedure for the rapid identification of EAggEC strains: first, screen E. coli strains with the clump formation test and subsequently perform the glass slide adherence assay to observe AA for confirmation.
Cite this paper: M. Fujioka, C. Ahsan and Y. Otomo, "Rapid Detection Method for Enteroaggregative Escherichia coli Using Simple Clump Formation and Aggregative Assay," Advances in Microbiology, Vol. 3 No. 8, 2013, pp. 552-556. doi: 10.4236/aim.2013.38074.
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