The study aimed to evaluate the instrumental and clinical properties of an innovative gel formulation for anti-aging treatment. This was an open, non-controlled study, where the eligible subjects, divided into three subgroups according to age, had to perform a single dose application of the gel on the face for a short-term evaluation and a 4-week repeated use, twice a day, for a long-term evaluation. Instrumental and clinical evaluations had to be performed mono-laterally at the level of the face (right or left side according to a predisposed randomization list) in basal conditions (T0), 20 minutes after the first dose application (T20min) and after 2 and 4 weeks of treatment (T2wks - T4wks). Thirty-three subjects completed the study showing, after only 20 minutes from the 1st product application, a clinically important and statistically significant improvement of crow’s feet, skin dullness clinical score and cutaneous microrelief clinical score (p < 0.05), after 2 weeks of treatment an improvement of skin tonicity (p < 0.05) and after 4 weeks of treatment having a significant decrease of nasolabial folds clinical score (p < 0.05). The study showed the great efficacy of an innovative anti-aging gel in reducing the skin roughness and skin dryness, improving the skin firmness and, in general, providing a global skin rejuvenation.
 I. Ghersetich, M. Troiano, V. De Giorgi and T. Lotti, “Receptors in Skin Ageing and Anti-Aging Agents,” Dermatologic Clinics, Vol. 25, No. 4, 2007, pp. 655-662. http://dx.doi.org/10.1016/j.det.2007.06.018
 G. J. Fisher, J. Varani and J. J. Voorhees, “Looking older: Fibroblast Collapse and Therapeutic Implications,” JAMA Dermatology, Vol. 144, No. 5, 2008, pp. 666-672. http://dx.doi.org/10.1001/ archderm.144.5.666
 M. Wlaschek, et al., “Solar UV Irradiation and Dermal Photoaging,” Journal of Photochemistry and Photobiology B: Biology, Vol. 63, No. 1-3, 2001, pp. 41-51. http://dx.doi.org/10.1016/S1011-1344 (01)00201-9
 W. Ma, et al., “Chronological Ageing and Photoageing of the Fibroblasts and the Dermal Connective Tissue,” Clinical and Experimental Dermatology, Vol. 26, No. 7, 2001, pp. 592-599.
 L. D. Muiznieks, A. S. Weiss and F. W. Keeley, “Structural Disorder and Dynamics of ELastin,” Biochemistry and Cell Biology, Vol. 88, No. 2, 2010, pp. 239-250. http://dx.doi.org/10.1139/O09-161
 F. W. Danby, “Nutrition and Aging Skin: Sugar and Glycation,” Clinics in Dermatology, Vol. 4, No. 28, 2010, pp. 409-411. http://dx.doi.org/10.1016/j.clindermatol.2010.03.018
 M. F. Demierre, et al., “Public Knowledge, Awareness, and Perceptions of the Association between Skin Aging and Smoking,” Journal of the American Academy of Dermatology, Vol. 41, No. 1, 1999, pp. 27-30. http://dx.doi.org/10.1016/S0190-9622(99)70401-9
 E. Makrantonaki, et al., “Skin and Brain Age Together: The Role of Hormones in the Ageing Process,” Experimental Gerontology, Vol. 45, No. 10, 2010, pp. 801-813. http://dx.doi.org/10.1016/j.exger. 2010.08.005
 US Food Drug Administration, “Alpha Hydroxy Acids in Cosmetics,” US Food Drug Administration, Silver Spring, 2013. http://www.fda.gov/Cosmetics/ProductandIngredientSafety/SelectedCosmetic Ingredients/ucm107940.htm
 A. Sparavigna, M. Setaro and A. Di Pietro, “Healthy Skin 2005: Results of Italian Study on Healthy Population with Particular Regard to the Ageing Phenomenon,” Journal of Plastic Dermatology, Vol. 2, 2006, pp. 23-29.
 T. Pavicic, et al., “Efficacy of Cream-Based Novel Formulations of Hyaluronic Acid of Different Molecular Weights in Anti-Wrinkle Treatment,” Journal of Drugs in Dermatology, Vol. 10, No. 9, 2011, pp. 990-1000.
 N. Philips, et al., “Direct Inhibition of Elastase and Matrixmetalloproteinases and Stimulation of Biosynthesis of Fibrillar Collagens, Elastin, and Fibrillins by Xanthohumol,” Journal of Cosmetic Science, Vol. 61, No. 2, 2010, 2006, pp. 125-132.