JWARP  Vol.5 No.12 , December 2013
Abatement of Helminth Eggs and Bacterial and Viral Indicators in Soil after Land Application of Treated Sludges
ABSTRACT

The reduction of helminth eggs, fecal coliforms and somatic coliphages present in sewage sludge after treatments and abatement by application to soil was determined. Traditional stabilization processes produced small changes in the concentrations of the parameters studied. In contrast, thermal treatments and liming produced dramatic reductions. Fecal coliforms were the most affected by both types of treatments; somatic coliphages showed some persistence after 30 minutes at 60°C; and both somatic coliphages and helminth eggs showed some persistence to storage in quick lime. However, both treatments supplied biosolid suitable for unrestricted application in agriculture. Abatement in soils in the climatic conditions tested (mild to cold temperatures and high relative humidity) was slow and took several months to reach the background levels. These results suggest that environmental conditions (temperature and humidity) play the main role in inactivating the microorganisms, since abatement was similar in different soil types. The extended permanence of pathogens and microbial indicators in soil after the application of treated sludges indicates that, in the normal weather conditions of the areas where the study was performed and the amounts of sludges applied, contaminant microbes are not easily mobilized from the complex matrixes that constitute the treated sludges and that consequently in normal conditions their release as diffuse pollution is of lesser importance.


Cite this paper
C. Campos, M. Beltrán, M. Duarte, L. Medina, F. Lucena and J. Jofre, "Abatement of Helminth Eggs and Bacterial and Viral Indicators in Soil after Land Application of Treated Sludges," Journal of Water Resource and Protection, Vol. 5 No. 12, 2013, pp. 1155-1164. doi: 10.4236/jwarp.2013.512122.
References
[1]   E. Epstein, “Pathogenic Health Aspects of Land Application,” BioCycle, Vol. 39, No. 6, 1998, pp. 62-67.

[2]   Journal Officiel, “Prescriptions Techniques Applicable aux Epandages de Boues sur sols Agricoles en Application du Decret na 97-1133,” Gouvernement de France, Paris, 1998.

[3]   European Community, “Working Document—Biological Treatment of Biowaste,” 2nd Draft, 2001.
http://europa.eu.int/comm/environment/waste/factsen.htm

[4]   USEPA, “Biosolids Technology Fact Sheet. Use of Land Filling for Biosolids Management,” United States Environmental Protection Agency, 2008, p. 103.

[5]   C. Gantzer, L. Gillerman, M. Kuznetsov and G. Oron, “Adsorption and Survival of Faecal Coliforms, Somatic Coliphages and F-Specific RNA Phages in Soil Irrigated with Wastewater,” Water Science and Technology, Vol. 43, No. 12, 2001, pp. 117-124.

[6]   L. Sahlstrom, “A Review of Survival of Pathogenic Bacteria in Organic Waste Used in Biogas Plants,” Bioresources Technology, Vol. 87, No. 2, 2003, pp. 161-166.

[7]   C. Guzmán, J. Jofre, M. Montemayor and F. Lucena, “Occurrence and Levels of Indicators and Selected Pathogens in Different Sludges and Biosolids,” Journal of Applied Microbiology, Vol. 103, No. 6, 2007, pp. 2420-2429.

[8]   J. Santamaria and G. A. Torzanzos, “Enteric Pathogens and Soil: A Short Review,” International Microbiology, Vol. 6, No. 1, 2003, pp. 5-9.

[9]   A. Pourcher, F. Picard-Bonnaud, V. Ferré, A. Gosinska, V. Stan and G. Moguedet, “Survival of Faecal Indicators and Enteroviruses in Soil after Land-Spreading of Municipal Sewage Sludge,” Applied Soil Ecology, Vol. 35, No. 3, 2007, pp. 473-479.

[10]   IAWPRC, Study Group on Health Related Water Microbiology, “Bacteriophages as Model Viruses in Water Quality Control,” Water Research, Vol. 25, No. 5, 1991, pp. 529-545.

[11]   P. Payment and E. Franco, “Clostridium perfringens and Somatic Coliphages as Indicators of the Efficiency of Drinking Water Treatment for Viruses and Protozoan Cysts,” Applied and Environmental Microbiology, Vol. 59, No. 8, 1993, pp. 2418-2424.

[12]   B. Jimenez, “Helminth Ova Control in Sludge: A Review,” Water Science and Technology, Vol. 56, No. 9, 2007, pp. 147-155.

[13]   B. Jiménez, C. Maya, E. Sánchez, A. Romero, L. Lira and J. A. Barrios, “Comparison of the Quantity and Quality of the Microbiological Content of Sludge in Countries with Low and High Content of Pathogens,” Water Science and Technology, Vol. 46, No. 10, 2002, pp. 17-24.

[14]   L. Mocé-Llivina, M. Muniesa, H. Pimenta-Vale, F. Lucena and J. Jofre, “Survival of Bacterial Indicator Species and Bacteriophages after Thermal Treatment of Sludge and Sewage,” Applied and Environmental Microbiology, Vol. 69, No. 3, 2003, pp. 1452-1456.

[15]   F. Lucena and J. Jofre, “Potential Use of Bacteriophages as Indicators of Water Quality and Wastewater Treatment Processes,” In: P. M. Sabour and M. W. Griffiths, Eds., Bacteriophages in the Control of Food- and Waterborne Pathogens, ASM Press, Washington DC, 2010, pp. 103-118.

[16]   C. Gantzer, P. Gaspard, L. Galvez, A. Huyard, N. Dumouthier and J. Schwartzbrod, “Monitoring of Bacterial and Parasitological Contamination during Various Treatment of Sludge,” Water Research, Vol. 16, No. 16, 2001, pp. 3763-3370.

[17]   P. Gessel, N. Hansen, S. Goyal, L. Johnston and J. Webb, “Persistence of Zoonotic Pathogens in Surface Soil Treated with Different Rates of Liquid Pig Manure,” Applied Soil Ecology, Vol. 25, No. 3, 2004, pp. 237-243.

[18]   WHO, “The Global Burden of Disease. 2004 Update,” World Health Organization, Geneva, 2008.
http://www.who.int/healthinfo/global_burden_disease/en/

[19]   A. Prüss-üstün and C. Corvalan, “Preventing Disease through Healthy Environments. Towards an Estimate of the Estimate of the Environmental Burden of Disease,” WHO Press, World Health Organization, Genev, 2006, p. 105.

[20]   J. Lasobras, J. Dellundé, J. Jofre and F. Lucena, “Occurrence and Levels of Phages Proposed as Surrogate Indicators of Enteric Viruses in Different Types of Sludges,” Journal of Applied Microbiology, Vol. 86, No. 4, 1999, pp. 723-729.

[21]   ISO, “ISO 10705-2: Water Quality—Detection and Enumeration of Bacteriophages—Part 2: Enumeration of Somatic Coliphages,” International Organisation for Standardisation, Geneva, 2000.

[22]   Norma Oficial Mexicana, “Lodos y Biosólidos. Especificaciones y Límites Máximos Permisibles de Contaminantes Para su Aprovechamiento y Disposición Final,” NOM-004-ECOL-2002, 2002, 61 p.

[23]   S. Astals, C. Venegas, M. Peces, J. Jofre, F. Lucena and J. Mata-Alvarez, “Balancing Hygienization and Anaerobic Digestion of Raw Sewage Sludge,” Water Research, Vol. 46, No. 19, 2012, pp. 6218-6227.

[24]   K. Cheung, M. Lang and S. Smith, “The Effects of Centrifugation Dewatering on Escherichia coli Numbers in Digested Sewage Sludge,” Proceeding of the Joint CIWEM Aqua Environmental Technology Transfer 8th European Biosolids and Organic Residuals Conference, Wakefield, 24-26 November 2003.

[25]   M. C. Monteleone, D. Furness, B. Jefferson and E. Cartmell, “Fate of E. coli across Mechanical Dewatering Processes,” Environmental Technology, Vol. 25, No. 7, 2004, pp. 825-831.

[26]   J. Jofre, “Is the Replication of Somatic Coliphages in Water Environments Significant?” Journal of Applied Microbiology, Vol. 106, No. 4, 2009, pp. 1059-1069.

[27]   B. Jimenez, C. Maya and G. Salgado, “The Elimination of Helminth Ova, Faecal Coliforms, Salmonella and Protozoan Cysts by Various Physicochemical Processes in Wastewater and Sludge,” Water Science and Technology, Vol. 43, No. 12, 2001, pp. 179-182.

[28]   P. Jatinder, J. P. S. Sdihu and S. G. Toze, “Human Pathogens and Their Indicators in Biosolids: A Literature Review,” Environmental International, Vol. 35, No. 2, 2009, pp. 187-201.

[29]   USEPA, “Environmental Regulations and Technology. Control of Pathogens and Vector Attraction in Sewage Sludge, Appendix F—EPA/625/R-92/013,” Office of Research and Development, 1999.

[30]   S. K. Spillmann, F. Traub, M. Schwyzer and R. Wyler, “Inactivation of Animal Viruses during Sewage Sludge Treatment,” Applied and Environmental Microbiology, Vol. 53, No. 9, 1987, pp. 2077-2081.

[31]   C. Bean, J. Hansen, A. Margolin, H. Balkin, G. Batzer and G. Widmer, “Class B Alkaline Stabilization to Achieve Pathogen Inactivation,” International Journal of Research in Public Health, Vol. 4, No. 1, 2007, pp. 53-60.

[32]   B. Mignotte-Cadiergues, C. Gantzer, L. Schwartzbrod, “Evaluation of Bacteriophages during the Treatment of Sludge,” Water Science and Technology, Vol. 46, No. 4, 2002, pp. 189-194.

[33]   R. Gibbs, C. Hu, G. Ho and I. Unkovich, “Regrowth of Faecal Coliforms and Salmonellae in Stored Biosolids and Soil Amended with Biosolids,” Water Science and Technology, Vol. 35, No. 11, 1996, pp. 269-275.

[34]   K. J. Zaleski, K. L. Josephson, C. Gerba and P. Pepper, “Potential Regrowth and Recolonization of Salmonellae and Indicators in Biosolids and Biosolid Amended Soil,” Applied and Environmental Microbiology, Vol. 71, No. 7, 2005, pp. 3701-3708.

 
 
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