AMPC  Vol.3 No.8 , December 2013
A Fluorescence Ratiometric Probe for Detection of Cyanide in Water Sample and Living Cells
ABSTRACT

In the present work, Compound 1 has been synthesized as a novel fluorescence ratiometric probe for CN-. Upon treatment with CN-, Probe 1 exhibited a fluorescence ratiometric response, with the emission wavelength shift from 570 nm to 608 nm. When 90 μM CN- was introduced, the emission ratios (I570/I608) of the probe changed dramatically from 0.52156 to 4.21472. The detection limit was also measured to be 0.24 μM (S/N = 3). In addition, Probe 1 had a selective response to CN-, while other anions caused nearly no interference. The sensing reaction product of Probe 1 with CN- was characterized by 1H NMR spectra and ESI Mass spectrometry. Furthermore, Probe 1 has been successfully applied to detect CN- in natural water samples. The fluorescence imaging experiments in living cells also demonstrated that Probe 1 could monitor CN- in biological samples.


Cite this paper
L. Long, L. Wang and Y. Wu, "A Fluorescence Ratiometric Probe for Detection of Cyanide in Water Sample and Living Cells," Advances in Materials Physics and Chemistry, Vol. 3 No. 8, 2013, pp. 307-313. doi: 10.4236/ampc.2013.38042.
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