OJST  Vol.3 No.9 A , December 2013
Fluid filtration and dye leakage testing of Resilon/Epiphany and guttapercha/Pulp Canal Sealer root canal fillings
Abstract: The aim of the present study was to compare sealing abilities of Resilon/Epiphany (R/E) with those of guttapercha and Kerr Pulp Canal Sealer EWT (GP/PCS). 50 single rooted teeth were randomly divided into 2 groups (n = 22) (R/E; GP/PCS), and 2 control groups (n = 3) (Positive, Negative). Fluid flow rate through the filled roots was measured using a fluid filtration device. The same specimens were immersed in a 2% Methylen Blue Solution. Roots were embedded in clear epoxy resin and cross sectionet at 1 mm intervals along the length of the root. Circumferential dye penetration was evaluated at 40× magnification. Fluid filtration of teeth obturated with the R/E system (0.085 [0.057, 0.113] μL·min-1), and dye penetration test showed no statistically significant difference compared to those obturated with GP/PCS (0.113 [0.057, 0.141] μL·min-1). Within the limits of the present study, there is no statistically significant difference between R/E and GP/PCS sealing abilities.
Cite this paper: Conte, G. , Mancini, M. , Cerroni, L. , Costantini, M. and Cianconi, L. (2013) Fluid filtration and dye leakage testing of Resilon/Epiphany and guttapercha/Pulp Canal Sealer root canal fillings. Open Journal of Stomatology, 3, 14-21. doi: 10.4236/ojst.2013.39A003.

[1]   Bergenholtz, G. (1974) Microorganisms from necrotic pulp of traumatized teeth. Odontologisk Revy, 25, 347-358.

[2]   Kakehashi, S., Stanley, H. and Fitzgerald, R. (1965) The effect of surgical exposure of dental pulps in ger-free and conventional laboratory rats. Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontology, 20, 340-349.

[3]   Moller, A.J., Fabricius, L., Dahlen, G., Ohman, A.E. and Heyden, G. (1981) Influence on periradicular tissues of indigenous oral bacteria and necrotic pulp tissue in monkeys. Scandinavian Journal of Dental Research, 89, 475-484.

[4]   Byström, A. and Sundqvist, G. (1983) Bacteriological evaluation of the effect of 0.5 percent sodium hypochlorite in endodontic therapy. Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontology, 55, 307-312.

[5]   Byström, A. and Sundqvist, G. (1985) The antibacterial action of sodium hypochlorite and EDTA in 60 cases of endodontic therapy. International Endodontic Journal, 18, 35-40.

[6]   Dalton, B.C., Ørstavik, D., Phillips, C., Pettiette, M. and Trope, M. (1998) Bacterial reduction with nickel-titanium rotary instrumentation. Journal of Endodontics, 24, 763-767.

[7]   Shuping, G.B., Ørstavik, D., Sigurdsson, A. and Trope, M. (2000) Reduction of intracanal bacteria using nickel-titanium rotary instrumentation and various medications. Journal of Endodontics, 26, 751-755.

[8]   Schilder, H. (1967) Filling root canals in three dimensions. Dental Clinics of North America, 11, 723-744.

[9]   Khayat, A., Lee, S.J. and Torabinejad, M. (1993) Human saliva penetration of coronally unsealed obturated root canals. Journal of Endodontics, 19, 458-461.

[10]   Madison, S. and Wilcox, L.R. (1988) An evaluation of coronal microleakage in endodontically treated teeth. Part III. In vivo study. Journal of Endodontics, 14, 455-458.

[11]   Swanson, K. and Madison, S. (1987) An evaluation of coronal microleakage in endodontically treated teeth. Part I. Time Periods. Journal of Endodontics, 13, 56-59.

[12]   Torabinejad, M., Ung, B. and Kettering, J.D. (1990) In Vitro bacterial penetration of coronally unsealed endodontically treated teeth. Journal of Endodontics, 16, 566-569.

[13]   Hovland, E.J. and Dumsha, Y.C. (1986) Leakage evaluation in vitro of the root canal sealer Sealapex. International Endodontic Journal, 19, 21-28.

[14]   Wu, M.K. and Wesselink, P.R. (1993) Endodontic leakage studies reconsidered, part 1: methodology, application and relevance. International Endodontic Journal, 26, 37-43.

[15]   Lee, K.W., Williams, M.C., Camps, J.J. and Pashley, D.H. (2002) Adhesion of endodontic sealers to dentin and gutta-percha. Journal of Endodontics, 28, 684-688.

[16]   Shipper, G. and Trope, M. (2004) In vitro microbial leakage of endodontically treated teeth using new and standard obturation techniques. Journal of Endodontics, 30, 154-158.

[17]   Amass, W., Amass, A. and Tighe, B. (1998) A review of biodegradable polymers: Uses, current developments in the synthesis and characterization of biodegradable polyesters, blend of biodegradable polymers and recent advances in biodegradation studies. Polymer International, 47, 89-144.<89::AID-PI86>3.0.CO;2-F

[18]   Jia, W.T. and Alpert, B. (2003) Root canal filling material. US Patent No. 20030113686.

[19]   Shipper, G., Ørstavik, D., Teixeira, F.B. and Trope, M. (2004) An evaluation of microbial leakage in roots filled with a thermoplastic synthetic polymer-based root canal filling material (Resilon). Journal of Endodontics, 30, 342-347.

[20]   Shipper, G., Teixeira, F.B., Arnold, R.R. and Trope, M. (2005) Periapical inflammation after coronal microbial inoculation of dogs roots filled with gutta-percha or resilon. Journal of Endodontics, 31, 91-96.

[21]   Teixeira, F.B., Teixeira, E.C.N., Thompson, J.Y. and Trope, M. (2004) Fracture resistance of roots endodontically treated with a new resin filling material. Journal of American Dental Association, 135, 646-652.

[22]   Antonopoulos, K.G., Attin, T. and Hellwig, E. (1998) Evaluation of the apical seal of root canal fillings with different methods. Journal of Endodontics, 24, 655-658.

[23]   Michailesco, P.M., Valcarel, J., Grieve, A.R., Levallois, B., Lerner, B. and Lerner, D. (1996) Bacterial leakage in endodontics: An improved method for quantification. Journal of Endodontics, 22, 535-539.

[24]   Haikel, Y., Wittenmeyer, W., Bateman, G., Bentaleb, A. and Allemann, C. (1999) A new method for the quantitative analysis of endodontic microleakage. Journal of Endodontics, 25, 172-178.

[25]   Kaya, B.U., Kececi, A.D. and Belli, S. (2007) Evaluation of the sealing ability of gutta-percha and thermoplastic synthetic polymer-based system along the root canals trough the glucose penetration model. Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontology, 104, 66-73.

[26]   Karagöz-Kücükay, I. and Bayirli, G. (1994) An apical leakage study in the presence and absence of the smear layer. International Endodontic Journal, 27, 87-93.

[27]   Camps, J. and Pashley, D. (2003) Reliability of the dye penetration studies. Journal of Endodontics, 29, 592-594.

[28]   Ramachandran Nair, P.N. (1997) Apical periodontitis: A dynamic encounter between root canal infection and host response. Periodontology, 13, 121-148.

[29]   Pommel, L., Jacquot, B. and Camps, J. (2001) Lack of correlation among three methods for evaluation of apical leakage. Journal of Endodontics, 27, 347-350.

[30]   Orucoglu, H., Sengun, A. and Yilmaz, N. (2005) Apical leakage of resin based root canal sealers with a new computerized fluid filtration meter. Journal of Endodontics, 31, 886-890.

[31]   Wu, M.K., De Gee, A.J., Wesselink, P.R. and Moorer, W.R. (1993) Fluid transport and bacterial penetration along root canals fillings. International Endodontic Journal, 26, 203-208.

[32]   Galvan, R.R., West, L.A., Liewehr, F.R. and Pashley, D.H. (2002) Coronal microleakage of five materials used to create an intracoronal seal in endodontically treated teeth. Journal of Endodontics, 28, 59-61.

[33]   Pitout, E., Oberholzer, T.G., Blignaut, E. and Molepo, J. (2006) Coronal leakage of teeth root filled with guttapercha or resilon root canal filling material. Journal of Endodontics, 32, 879-881.

[34]   Wu, M.K., De Gee, A.J. and Wesselink, P.R. (1994) Fluid transport and dye penetration along root canal fillings. International Endodontic Journal, 27, 233-238.

[35]   Wu, M.K., De Gee, A.J. and Boersma, J. (1995) A 1-year follow up study on leakage of four root canal sealers at different thickness. International Endodontic Journal, 28, 185-189.

[36]   Biggs, S.G., Knowles, K.I., Ibarrola, J.L. and Pashley, D.H. (2006) An in vitro assessment of the sealing ability of Resilon/Epiphany using fluid filtration. Journal of Endodontics, 32, 759-761.

[37]   Onay et, E.O., Ungor, M. and Orucoglu, H. (2006) An in vitro evaluation of the apical sealing ability of a new resin based root canal obturation system. Journal of Endodontics, 32, 976-978.

[38]   Paqué, F. and Sirtes, G. (2007) Apical sealing ability of Resilon/Epiphany versus gutta-percha/AH Plus: Immediate and 16-months leakage. Internationa Endodontic Journal, 40, 722-729.

[39]   Sagsen, B., Kahraman, Y. and Orucoglu, H. (2006) Evaluation of microleakage of roots filled with different techniques with a computerized fluid filtration technique. Journal of Endodontics, 32, 1168-1170.

[40]   Tunga, U. and Bodrumlu, E. (2006) Assessment of the sealing ability of a new root canal obturation material. Journal of Endodontics, 32, 876-878.

[41]   Wedding, J.R., Brown, C.E., Legan, J.J., Moore, B.K. and Vail, M.M. (2007) An in vitro comparison of microleakage between Resilon and Gutta-perhca with a fluid filtration model. Journal of Endodontics, 33, 1447-1449.

[42]   Baumgartner, G., Zehnder, M. and Paque, F. (2007) Enterococcus faecalis type strain leakage through root canals filled with Gutta-Percha/AH plus or Resilon/Epiphany. Journal of Endodontics, 33, 45-47.

[43]   De-Deus, G., Audi, C., Murad, C., Fidel, S. and Fidel, R.A. (2007) Sealing ability of oval-shaped canals filled using the system B heat source with either Gutta-percha or Resilon: An ex vivo study using a polymicrobial leakage model. Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontology, 104, 114-119.

[44]   Munoz, H.R., Saravia-Lemus, A., Florian, W.E. and Lainfiesta, J.F. (2007) Microbial leakage of Enterococus faecalis after post space preparation in teeth filled in vivo with realseal versus gutta-percha. Journal of Endodontics, 33, 673-675.

[45]   Cobankara, F.K., Adanir, N. and Belli, S. (2004) Evaluation of the influence of the smear layer on the apical and coronal sealing abilites of two sealers. Journal of Endodontics, 30, 406-409.

[46]   Raina, R., Loushine, R.J., Weller, N., Tay, F.R., Pashley, D.H. (2007) Evaluation of the quality of the apical seal in Resilon/Epiphany and Gutta-Percha/AH plus-filled root canals by using a fluid filtration approach. Journal of Endodontics, 33, 944-947.

[47]   Tay, F.R., Hiraishi, N. and Pashley, D.H. (2006) Bondability of Resilon to a methacrylate based root canal sealer. Journal of Endodontics, 32, 133-137.

[48]   Tay, F.R., Loushine, R.J., Lambrechts, P., Weller, N. and Pashley, D.H. (2005) Geometric factors affecting dentin bonding in root canals: A theoretical modeling approach. Journal of Endodontics, 31, 584-589.

[49]   Tay, F.R., Loushine, R.J., Weller, R.N., Kimbrough, W. F., Pashley, D.H., Mak, Y.F., Lai, C.N., et al. (2005) Ultrastructural evaluation of the apical seal in roots filled with a polycaprolactone-based root canal filling material. Journal of Endodontics, 32, 514-519.

[50]   Perdigao, J., Lopes, M.M. and Gomes, G. (2007) Interfacial adaptation of adhesive materials to root canal dentin. Journal of Endodontics, 33, 259-263.

[51]   Bouillaguet, S., Troesch, S., Wataha, J.C., Krejci, I., Meyer, J.M. and Pashley, D.H. (2003) Microtensile bond strength between adhesive cements and root canal dentin. Dental Materials, 19, 199-205.

[52]   Hiraishi, N., Papacchini, F. and Loushine, R.J. (2005) Shear bond strength of Resilon to a methacrylate-based root canal sealer. International Endodontic Journal, 38, 753-763.

[53]   Gesi, A., Raffaelli, O., Goracci, C., Pashley, D.H., Tay, F.R. and Ferrari, M. (2005) Interfacial strength of Resilon and gutta-percha to intraradicular dentin. Journal of Endodontics, 31, 809-813.

[54]   De-Deus, G., Namen, F. and Galan Jr., J. (2008) Reduced long-term sealing ability of adhesive root fillings after water-storage stress. Journal of Endodontics, 34, 322-325.

[55]   Gan, Z., Liang, Q., Zhang, J. and Jing, X. (1997) Enzymatic degradation of poly(_-caprolactone) film in phosphate buffer solution containing lipases. Polymer Degradation and Stability, 56, 209-213.

[56]   Hayashi, T., Nakayama, K., Mochizuki, M. and Masuda, T. (2002) Studies on biodegradable poly(hexano-6-lactone) fibers. Part 3. Enzymatic degradation in vitro (IUPAC Technical Report). Pure and Applied Chemistry, 74, 869-880.

[57]   Lefèvre, C., Tidjani, A., Vander Wauven, C. and David, C. (2002) The interaction between microorganisms and substrate in the biodegradation of polycaprolactone. Journal of Applied Polymer Science, 83, 1334-1340.