Flavonoids are the
most widely used in the field of the food, medicine, and cosmetic due to their
pharmacological functions and biological activities, such as anti-tumor,
anti-inflammatory and antioxidation. This work described the assignment of
purity values to 3 flavonoids certified reference materials (CRMs) including
baicalein, 5,7-dihydoxy flavones and
naringenine which were developed in this study according to the ISO Guides 34
and 35. The qualitative analysis was performed
by liquid chromatography-mass spectrometry (LC-MS) and infrared
spectroscopy (IR). The CRMs’ purity values were assigned based on the weighted
average of quantitative nuclear magnetic resonance method and mass balance
approach with high resolution liquid chromatography-VWD. All the three CRMs
with following value mass fractions: baicalein at a certified purity P ± U (k = 2) of 98.8% ± 0.8%; 5,7-dihydoxy
flavones of 99.1% ± 0.7% and naringenine of 99.5% ± 1.0% respectively. The
homogeneity of the CRMs was determined by an in-house validated liquid
chromatographic method. Potential degradation during storage was also
investigated and a shelf-life based on this value was established.
Cite this paper
Quan, C. , Yao, H. and Hou, C. (2013) Certification and uncertainty evaluation of flavonoids certified reference materials. Agricultural Sciences
, 89-96. doi: 10.4236/as.2013.49B016
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