AiM  Vol.3 No.7 , November 2013
Mycelial Growth of Paecilomyces hepiali in Various Agar Media and Yield of Fruit Bodies in Rice Based Media
ABSTRACT
Growth of Paecilomyces hepiali in various agar media and yield of fruit bodies in rice based media were studied. The best growth in agar media was obtained at 25 (61.86 mm colony diameter in 14 days). The initial agar media pH range from 6 to 8 was found to be the most favourable for mycelial growth. This study found that agars made with powders of cereal grains alone do not support good mycelial growth of P. hepiali. Addition of peptone improved mycelial growth significantly. The most favourable carbon sources were Mannose, Fructose and Glucose. Organic nitrogen sources were found to be the most preferred. The results demonstrated that brown rice is better than polished rice in yield of fruit bodies. Addition of peptone was found to be quite significant in enhancing yield of fruit bodies. Peptone, as a supplement, gave a better yield than addition of egg yolk, albumen and a mixture of the two. The medium with 40 g brown rice, 0.325 g glucose, 0.65 g sucrose, 2 g peptone and 65 ml corn steep liquor was found to be the most favourable and it yielded 19.3 g of fresh fruit bodies.

Cite this paper
A. Chioza and S. Ohga, "Mycelial Growth of Paecilomyces hepiali in Various Agar Media and Yield of Fruit Bodies in Rice Based Media," Advances in Microbiology, Vol. 3 No. 7, 2013, pp. 529-536. doi: 10.4236/aim.2013.37071.
References
[1]   J.-S. Zhu, L. Gao, X.-H. Li, Y.-S. Yao, J.-Q. Zhao, Y.-J. Zhou and J.-H. Lu, “Maturational Alteration of Opposite- ly Orientated rDNA and Differential Proliferation of GC- and AT-biased Genotypes of Ophiocordyceps sinensis and Paecilomyces hepiali in Natural Cordyceps sinensis,” American Journal of Biomedical Sciences, Vol. 2, No. 3, 2010, pp. 217-238.
http://dx.doi.org/10.5099/aj100300217

[2]   G. S. Barseghyan, J. C. Holliday, T. C. Price, L. M. Ma- dison and S. P. Wasser, “Growth and Cultural-Morpho- logical Characteristics of Vegetative Mycelia of Medici- nal Caterpillar Fungus Ophiocordyceps sinensis G.H. Sung et al. (Ascomycetes) Isolates from Tibetan Plateau (P.R. China),” International Journal of Medicinal Mushrooms, Vol. 13, No. 6, 2011, pp. 565-581.
http://dx.doi.org/10.1615/IntJMedMushr.v13.i6.90

[3]   G.-H. Sung, N. L. Hywel-Jones, J.-M. Sung, J. J. Luang- sa-ard, B. Shrestha and J. W. Spatafora, “Phylogenetic Classification of Cordyceps and the Clavicipitaceous Fungi,” Studies in Mycology, Vol. 57, No. 1, 2007, pp. 5-59. http://dx.doi.org/10.3114/sim.2007.57.01

[4]   Y.-Q. Chen, N. Wang, L.-H. Qu, T.-H. Li, W.-M. Zhang, “Determination of the Anamorph of Cordyceps Sinensis Inferred from Analysis of the Ribosomal DNA Internal Transcribed Spacers and 5.8S rDNA,” Biochemical Sys- tematics and Ecology, Vol. 29, No. 6, 2001, pp. 597-607.
http://dx.doi.org/10.1016/S0305-1978(00)00100-9

[5]   Z.-Y. Liu, Y.-J. Yao, Z.-Q. Liang, A.-Y. Liu, D. N. Pe- gler and M. W. Chase, “Molecular Evidence for Anamor- ph-Teleomorph Connection in Cordyceps sinensis,” My- cological Research, Vol. 105, No. 7, 2001, pp. 827-832.
http://dx.doi.org/10.1017/S095375620100377X

[6]   Z.-Y. Liu, Z.-Q. Liang, A.-Y. Liu, Y.-J. Yao, K. D. Hyde and Z.-N. Yu, “Molecular Evidence for Teleomorph-Ana- morph Connections in Cordyceps Based on ITS-5±8S rDNA Sequences,” Mycological Research, Vol. 106, No. 9, 2002, pp. 1100-1108.
http://dx.doi.org/10.1017/S0953756202006378

[7]   A. K. Bhandari, J. S. Negi, V. K. Bisht, M. K. Bharti and N. Singh, “Chemical Constituent, Inorganic Elements and Properties of Cordyceps sinensis—A Review,” Nature and Science, Vol. 8, No. 9, 2010.

[8]   S.-J. Yu, Y. Zhang, C.-R. Li, Q. Zhang, Z.-Y. Ma and M.- Z. Fan, “Optimization of Ultrasonic Extraction of Myce- lial Polysaccharides from Paecilomyces hepiali Using Re- sponse Surface Methodology and Its Antioxidant Activ- ity,” African Journal of Biotechnology, Vol. 10, No. 75, 2011, pp. 17241-17250.

[9]   A. Thakur, R. Hui, Z. Hongyan, Y. Tian, C. Tianjun and C. Mingwei, “Pro-Apoptotic Effects of Paecilomyces he- piali, a Cordyceps sinensis Extract on Human Lung Ade- nocarcinoma A549 Cells in Vitro,” Journal of Cancer Re- search and Therapeutics, Vol. 7, No. 4, 2011, pp. 421-426. http://dx.doi.org/10.4103/0973-1482.92007

[10]   P. Stamets, “Growing Gourmet and Medicinal Mushrooms,” 3rd Edition, Ten Speed Press, California, 2000, 87 p.

[11]   J. C. Holliday, P. Cleaver, M. Loomis-Powers and D. Pa- tel, “Analysis of Quality and Techniques for Hybridiza- tion of Medicinal Fungus Cordyceps sinensis (Berk.) Sacc. (Ascomycetes),” International Journal of Medicinal Mushrooms, Vol. 6, No. 2, 2004, pp. 151-164.
http://dx.doi.org/10.1615/IntJMedMushr.v6.i2.60

[12]   “New Chapter Inc. LifeShield Cordyceps,” 2013.
http://www.newchapter.com/mushrooms/lifeshield-cordyceps

[13]   A. Imtiaj, C. Jayasinghe, G. W. Lee and T. Lee, “Compa- rative Study of Environmental and Nutritional Factors on the Mycelial Growth of Edible Mushrooms,” Journal of Culture Collections, Vol. 6, 2008, pp. 97-105.
http://www.nbimcc.org/JCC/2009/JCC09612/JCC09612S.htm

[14]   S. M. Shim, K. R. Lee, S. H. Kim, K. H. Im, J. W. Kim, U. Y. Lee, J. O. Shim, M. W. Lee and T. S. Lee, “The Op- timal Culture Conditions Affecting the Mycelial Growth and Fruiting Body Formation of Paecilomyces fumoso- roseus,” Mycobiology, Vol. 31, No. 4, 2003, pp. 214-220.
http://dx.doi.org/10.4489/MYCO.2003.31.4.214

[15]   B. Yang and S. Ohga, “Growth Characteristic and Polysa- ccharide Content of Cordyceps sobolifera,” Journal of Food Function, Vol. 4, No. 1, 2008, pp. 1-6.

[16]   F. Sasaki, T. Miyamoto, Y. Tamai and T. Yajima, “Opti- mum Temperature and pH for Mycelial Growth of Cor- dyceps nutans Pat. (Ascomycetes),” International Journal of Medicinal Mushrooms, Vol. 7, No. 1-2, 2005, pp. 301- 304. http://dx.doi.org/10.1615/IntJMedMushr.v7.i12.270

[17]   G. Sung, B. Shrestha, S. Han and J. Sung, “Growth and Cultural Characteristics of Ophiocordyceps longissima Col- lected from Korea,” Mycobiology, Vol. 39, No. 2, 2011, pp. 85-91.
http://dx.doi.org/10.4489/MYCO.2011.39.2.085

[18]   G. Sung, B. Shrestha, S. Han and J. Sung, “Cultural Char- acteristics of Ophiocordyceps heteropoda Collected from Korea,” Mycobiology, Vol. 39, No. 1, 2011, pp. 1-6.
http://dx.doi.org/10.4489/MYCO.2011.39.1.001

[19]   G. Sung, B. Shrestha, S. Han, S. Kim and J. Sung, “Growth and Cultural Characteristics of Cordyceps cardinalis Col- lected from Korea,” Mycobiology, Vol. 38, No. 4, 2010, pp. 274-281.
http://dx.doi.org/10.4489/MYCO.2010.38.4.274

[20]   S. M. R. Amin, N. Alam, M. Tania and M. A. Khan, “Study of Mycelial Growth of Cordyceps sinensis on Dif- ferent Culture Media, at Different pH Level and Tempe- rapture,” Bangladesh Journal of Mushroom, Vol. 2, No. 2, 2008, pp. 43-48.

[21]   C.-H. Dong and Y.-J. Yao, “Nutritional Requirements of Mycelial Growth of Cordyceps sinensis in Submerged Culture,” Journal of Applied Microbiology, Vol. 99, 2005, pp. 483-492.
http://dx.doi.org/10.1111/j.1365-2672.2005.02640.x

[22]   D. H. Jennings and G. Lysek, “Fungal Biology: Under- standing the Fungal Lifestyle,” 2nd Edition, BIOS Scien- tific Publishers Ltd., New York, 1999, p. 45.

[23]   P. G. Miles and S. T. Chang, “Mushroom Biology—Con- cise Basics and Current Developments,” World Scientific, 1997, p. 41.

[24]   S. C. Sati and S. Bisht, “Utilization of Various Carbon Sources for the Growth of Waterborne Conidial Fungi,” Mycologia, Vol. 98, No. 5, 2006, pp. 678-681.
http://dx.doi.org/10.3852/mycologia.98.5.678

[25]   S.-Y. Kim, B. Shrestha, G.-H. Sung, S.-K. Han and J.-M. Sun, “Optimum Conditions for Artificial Fruiting Body Formation of Cordyceps cardinalis,” Mycobiology, Vol. 38, No. 2, 2010, pp. 133-136.
http://dx.doi.org/10.4489/MYCO.2010.38.2.133

[26]   P. D. Babu, R. S. Subhasree, R. Bhakyaraj and R. Vidhy- alakshmi, “Brown Rice-Beyond the Color Reviving a Lost Health Food—A Review,” American-Eurasian Journal of Agronomy, Vol. 2, No. 2, 2009, pp. 67-72.

 
 
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