JCDSA  Vol.3 No.3 B , November 2013
Impact of Celosia cristata Extract on Adipogenesis of Native Human CD34+/CD31- Cells
Abstract: Background: The adipose tissue mainly consists of adipocytes but also contains non-adipose cells. Among them, progenitor cells represent a local pool of immature cells that, in vitro, can undergo various lineage differentiation processes. These cells are thought to contribute to normal homeostasis of the adipose tissue through adipogenesis but also to the growth of the adipose tissue under chronic energy overload. The aim of the present study is to evaluate in vitro the capacity of a Celosia cristata extract to impact the adipogenic potential of native human adipose tissue progenitor cells, i.e. commitment and differentiation towards adipogenic lineage. Methods: Native adipose tissue progenitor cells were isolated by immunoselection/depletion approaches from human subcutaneous adipose tissues. Two distinct cell culture conditions were used to assess the effect of Celosia cristata extract on commitment and differenciation of progenitor cells. Cells were cultured either in differentiation medium for 10 days in the presence/absence of Celosia cristata extracts to study the impact on differentiation or first cultured in a commitment-inducing medium, with or without Celosia cristata extract, for 48 h and then cultured 10 days in differentiation medium to assess the impact on commitment. In both experimental series, the fate of progenitor cells was studied by quantification of lipids and by determining the expression of key genes involved in adipogenesis. Results: Data show that Celosia cristata extract reduces lipid content of progenitor cells undergoing differentiation. This reduction correlates with a reduced expression of C/EBPα. When progenitor cells are placed in commitment-inducing conditions, Celosia cristata extract induces a more potent reduction of lipid content. This reduction correlates with a decrease in the expression levels of master genes involved in adipogenesis: the genes of transcription factors PPARγ2 and C/EBPα as well as marker genes coding for LPL and GPDH. Conclusions: Celosia cristata extract decreases adipogenesis. The effect of the extract is stronger when studying commitment and differentiation than differentiation alone; it suggests that the extract impact the commitment of human adipose tissue progenitor cells.
Cite this paper: R. Fitoussi, D. Estève, A. Delassus and K. Vié, "Impact of Celosia cristata Extract on Adipogenesis of Native Human CD34+/CD31- Cells," Journal of Cosmetics, Dermatological Sciences and Applications, Vol. 3 No. 3, 2013, pp. 55-63. doi: 10.4236/jcdsa.2013.33A2013.

[1]   T. Tchkonia, T. Thomou, Y. Zhu, I. Karagiannides, C. Pothoulakis, M. D. Jensen and J. L. Kirkland, “Mechanisms and Metabolic Implications of Regional Differences among Fat Depots,” Cell Metabolism, Vol. 17, No. 5, 2013, pp. 644-656.

[2]   A. Miranville, C. Heeschen, C. Sengenès, C. A. Curat, R. Busse and A. Bouloumié, “Improvement of Postnatal Neovascularization by Human Adipose Tissue-Derived Stem Cells,” Circulation, Vol. 110, No. 3, 2004, pp. 349-355.

[3]   C. Sengenès, K. Lolmède, A. Zakaroff-Girard, R. Busse and A. Bouloumié, “Preadipocytes in the Human Subcutaneous Adipose Tissue Display Distinct Features from the Adult Mesenchymal and Hematopoietic Stem Cells,” Journal of Cellular Physiology, Vol. 205, No. 1, 2005, pp. 114-122.

[4]   J. M. Gimble, B. A. Bunnell, E. S. Chiu and F. Guilak, “Concise Review: Adipose-Derived Stromal Vascular Fraction Cells and Stem Cells: Let’s Not Get Lost in Translation,” Stem Cells, Vol. 29, No. 5, 2011, pp. 749-754.

[5]   A. Cignarelli, S. Perrini, R. Ficarella, A. Peschechera, P. Nigro and F. Giorgino, “Human Adipose Tissue Stem Cells: Relevance in the Pathophysiology of Obesity and Metabolic Diseases and Therapeutic Applications,” Expert Reviews in Molecular Medicine, Vol. 14, 2012, p. e19. 1017/erm.2012.13

[6]   J. K. Fraser, R. Schreiber, B. Strem, M. Zhu, Z. Alfonso, I. Wulur and M. H. Hedrick, “Plasticity of Human Adipose Stem Cells toward Endothelial Cells and Cardiomyocytes,” Nature Clinical Practice Cardiovascular Medicine, Vol. 3, Suppl. 1, 2006, pp. S33-S37. cardio0444

[7]   P. A. Zuk, M. Zhu, P. Ashjian, D. A. De Ugarte, J. I. Huang, H. Mizuno, Z. C. Alfonso, J. K. Fraser, P. Benhaim and M. H. Hedrick, “Human Adipose Tissue Is a Source of Multipotent Stem Cells,” Molecular Biology of the Cell, Vol. 13, No. 12, 2002, pp. 4279-4295.

[8]   Y. D. Halvorsen, D. Franklin, A. L. Bond, D. C. Hitt, C. Auchter, A. L. Boskey, E. P. Paschalis, W. O. Wilkison and J. M. Gimble, “Extracellular Matrix Mineralization and Osteoblast Gene Expression by Human Adipose Tissue-Derived Stromal Cells,” Tissue Engineering, Vol. 7, No. 6, 2001, pp. 729-741.

[9]   K. L. Spalding, E. Arner, P. O. Westermark, S. Bernard, B. A. Buchholz, O. Bergmann, L. Blomqvist, J. Hoffstedt, E. Naslund, T. Britton, H. Concha, M. Hassan, M. Ryden, J. Frisen and P. Arner, “Dynamics of Fat Cell Turnover in Humans,” Nature, Vol. 453, No. 7196, 2008, pp. 783-787.

[10]   Y. Macotela, B. Emanuelli, M. A. Mori, S. Gesta, T. J. Schulz, Y. H. Tseng and C. R. Kahn, “Intrinsic Differences in Adipocyte Precursor Cells from Different White Fat Depots,” Diabetes, Vol. 61, No. 7, 2012, pp. 1691-1699.

[11]   E. D. Rosen and O. A. MacDougald, “Adipocyte Differentiation from the Inside Out,” Nature Reviews Molecular Cell Biology, Vol. 7, No. 12, 2006, pp. 885-896.

[12]   Q. Q. Tang and M. D. Lane, “Adipogenesis: From Stem Cell to Adipocyte,” Annual Review of Biochemistry, Vol. 81, No. 1, 2012, pp. 715-736.

[13]   J. Sohle, A. Knott, U. Holtzmann, R. Siegner, E. Gronniger, A. Schepky, S. Gallinat, H. Wenck, F. Stab and M. Winnefeld, “White Tea Extract Induces Lipolytic Activity and Inhibits Adipogenesis in Human Subcutaneous (Pre)-Adipocytes,” Nutrition & Metabolism, Vol. 6, No. 1, 2009, p. 20.

[14]   S. Rayalam, M. A. Della-Fera and C. A. Baile, “Phytochemicals and Regulation of the Adipocyte Life Cycle,” Journal of Nutritional Biochemistry, Vol. 19, No. 11, 2008, pp. 717-726. 10.1016/j.jnutbio.2007.12.007

[15]   N. Gooda Sahib, N. Saari, A. Ismail, A. Khatib, F. Mahomoodally and A. Abdul Hamid, “Plants’ Metabolites as Potential Antiobesity Agents,” The Scientific World Journal, 2012, Article ID: 436039.

[16]   R. Ratnawati, M. R. Indra and A. Satuman, “Epigallocatechin Gallate of Green Tea Inhibits Proliferation, Differentiation and TNF-α in the Primary Human Visceral Preadipocytes Culture,” Majalah Llmu Faal Indonesia, Vol. 6, No. 3, 2007, pp. 160-168.

[17]   P. Tontonoz and B. M. Spiegelman, “Fat and Beyond: The Diverse Biology of PPARgamma,” Annual Review of Biochemistry, Vol. 77, 2008, pp. 289-312. biochem.77.061307.091829

[18]   R. Siersbaek, R. Nielsen and S. Mandrup, “PPARgamma in Adipocyte Differentiation and Metabolism—Novel Insights from Genome-Wide Studies,” FEBS Letters, Vol. 584, No. 15, 2010, pp. 3242-3249.

[19]   Z. Dudhia, J. Louw, C. Muller, E. Joubert, D. de Beer, C. Kinnear and C. Pheiffer, “Cyclopia maculata and Cyclopia subternata (Honeybush Tea) Inhibits Adipogenesis in 3T3-L1 Pre-Adipocytes,” Phytomedicine, Vol. 20, No. 5, 2013, pp. 401-408.

[20]   C. H. Jung, S. J. Jang, J. Ahn, S. Y. Gwon, T. I. Jeon, T. W. Kim and T. Y. Ha, “Alpinia officinarum Inhibits Adipocyte Differentiation and High-Fat Diet-Induced Obesity in Mice through Regulation of Adipogenesis and Lipogenesis,” Journal of Medicinal Food, Vol. 15, No. 11, 2012, pp. 959-967.

[21]   U. H. Park, J. C. Jeong, J. S. Jang, M. R. Sung, H. Youn, S. J. Lee, E. J. Kim and S. J. Um, “Negative Regulation of Adipogenesis by Kaempferol, a Component of Rhizoma Polygonati falcatum in 3T3-L1 Cells,” Biological & Pharmaceutical Bulletin, Vol. 35, No. 9, 2012, pp. 1525-1533.

[22]   S. P. Poulos, M. V. Dodson and G. J. Hausman, “Cell Line Models for Differentiation: Preadipocytes and Adipocytes,” Experimental Biology and Medicine, Vol. 235, No. 10, 2010, pp. 1185-1193.