AiM  Vol.3 No.7 , November 2013
Microbiological Contamination of Bed Linen and Staff Uniforms in a Hospital
ABSTRACT


Hospital linen is clearly recognized as a potential reservoir for microorganisms and could be a vector of disease transmission. The aim of this study was to isolate, count and identify fungi and bacteria from different kinds of clean and dirty linen in a hospital. Microbiological samples have been collected on clean bed linen (n = 200), dirty bed linen (n = 192) and staff uniforms (n = 192) by using contact plates. 55% of samples from clean bed linen were contaminated before contact with the patient, with a mean count of 3 cfu/25 cm2 (range: 1-117 cfu) when contaminated. Virtually all samples from dirty bed linen carried microorganisms, with a mean count of 23 cfu/25 cm2 (range 1-191 cfu). In addition, staff hospital uniforms showed the highest contamination rates in the study, with an average of 45 cfu/25 cm2 (range: 1-218 cfu). Microbial species were mostly bacteria commonly found in the environment or on human skin, such as staphylococci or micrococci. Nevertheless, 57% of the identified species may be opportunistic pathogens for humans, representing a risk for people with a deficient or weakened immune system, especially in cases of superinfection. Since contamination of linen seems to occur after washing, actively antimicrobial textiles would represent a valuable measure to prevent textiles from being a vehicle for transfer of microorganisms.



Cite this paper
A. Pinon, J. Gachet, V. Alexandre, S. Decherf and M. Vialette, "Microbiological Contamination of Bed Linen and Staff Uniforms in a Hospital," Advances in Microbiology, Vol. 3 No. 7, 2013, pp. 515-519. doi: 10.4236/aim.2013.37069.
References
[1]   R. M. Klevens, J. R. Edwards, C. L. Richards, et al., “Estimating Health Care-Associated Infections and Deaths in US Hospitals, 2002,” Public Health Reports, Vol. 122, No. 2, 2007, pp. 160-166.

[2]   European Center for Disease Prevention and Control, “Annual Epidemiological Report on Communicable Diseases in Europe 2008,” Stockholm, 2008.

[3]   I. Youngster, M. Berkovitch, E. Heyman, Z. Lazarovitch and M. Goldman, “The Stethoscope as a Vector of Infectious Diseases in the Pediatric Division” Acta Paediatrica, Vol. 97, No. 9, 2008, pp. 1253-1255.
http://dx.doi.org/10.1111/j.1651-2227.2008.00906.x

[4]   M. E. Zuliani Maluf, A. F. Maldonado, M. E. Bercial and S. A. Pedroso, “Stethoscope: A Friend or an Enemy?” Sao Paulo Medical Journal, Vol. 120, No. 1, 2002, pp. 13-15.

[5]   S. O. Teng, W. S. Lee, T. Y. Ou, Y. C. Hsieh, W. C. Lee and Y. C. Lin, “Bacterial Contamination of Patients’ Medical Charts in a Surgical Ward and the Intensive Care Unit: Impact on Nosocomial Infections,” Journal of Microbiology, Immunology and Infection, Vol. 42, No. 1, 2009, pp. 86-91.

[6]   A. N. Neely and M. P. Maley, “Survival of Enterococci and Staphylococci on Hospital Fabrics and Plastic,” Journal of Clinical Microbiology, Vol. 38, No. 2, 2000, pp. 724-726.

[7]   A. N. Neely, “A Survey of Gram-Negative Bacteria Survival on Hospital Fabrics and Plastics,” Journal of Burn Care & Rehabilitation, Vol. 21, No. 6, 2000, pp. 523-527.
http://dx.doi.org/10.1097/00004630-200021060-00009

[8]   M. Srinivasan, A. Uma, A. Vinodhkumaradithyaa, S. Gomathi and P. Thirumalaikolundusubramanian, “The Medical Overcoat—Is It a Transmitting Agent for Bacterial Pathogens?” Japanese Journal of Infectious Diseases, Vol. 60, No. 2-3, 2007, pp. 121-122.

[9]   A. M. Treakle, K. A. Thom, J. P. Furuno, S. M. Strauss, A. D. Harris and E. N. Perencevich, “Bacterial Contamination of Health Care Workers’ White Coats,” American Journal of Infection Control, Vol. 37, No. 2, 2009, pp. 101-105. http://dx.doi.org/10.1016/j.ajic.2008.03.009

[10]   W. Loh, V. V. Ng and J. Holton, “Bacterial Flora on the White Coats of Medical Students,” Journal of Hospital Infection, Vol. 45, No. 1, 2000, pp. 65-68.
http://dx.doi.org/10.1053/jhin.1999.0702

[11]   C. Perry, R. Marshall and E. Jones, “Bacterial Contamination of Uniforms,” Journal of Hospital Infection, Vol. 48, No. 3, 2001, pp. 238-241.
http://dx.doi.org/10.1053/jhin.2001.0962

[12]   E. Yamaguchi, F. Valena, S. M. Smith, A. Simmons and R. H. Eng, “Colonization Pattern of Vancomycin-Resistant Enterococcus faecium,” American Journal of Infection Control, Vol. 22, No. 4, 1994, pp. 202-206.
http://dx.doi.org/10.1016/0196-6553(94)90068-X

[13]   J. A. Wilson, H. P. Loveday, P. N. Hoffman and R. J. Pratt, “Uniform: An Evidence Review of the Microbiological Significance of Uniforms and Uniform Policy in the Prevention and Control of Healthcare-Associated Infections. Report to the Department of Health (England),” Journal of Hospital Infection, Vol. 66, No. 4, 2007, pp. 301-307. http://dx.doi.org/10.1016/j.jhin.2007.03.026

[14]   J. M. Boyce, G. Potter-Bynoe, C. Chenevert and T. King, “Environmental Contamination Due to Methicillin-Resistant Staphylococcus aureus: Possible Infection Control Implications,” Infection Control and Hospital Epidemiology, Vol. 18, No. 9, 1997, pp. 622-627.
http://dx.doi.org/10.1086/647686

[15]   R Development Core Team, “R: A Language and Environment for Statistical Computing,” R Foundation for Statistical Computing, Vienna, 2010.

[16]   D. Wong, K. Nye and P. Hollis, “Microbial Flora on Doctors’ White Coats,” British Medical Journal, Vol. 303, No. 6817, 1991, pp. 1602-1604.
http://dx.doi.org/10.1136/bmj.303.6817.1602

[17]   S. Malnick, R. Bardenstein, M. Huszar, J. Gabbay and G. Borkow, “Pyjamas and Sheets as a Potential Source of Nosocomial Pathogens,” Journal of Hospital Infection, Vol. 70, No. 1, 2008, pp. 89-92.
http://dx.doi.org/10.1016/j.jhin.2008.05.021

[18]   M. Burden, L. Cervantes, D. Weed, A. Keniston, C. S. Price and R. K. Albert, “Newly Cleaned Physician Uniforms and Infrequently Washed White Coats Have Similar Rates of Bacterial Contamination after an 8-Hour Workday: A Randomized Controlled Trial,” Journal of Hospital Medicine, Vol. 6, No. 4, 2011, pp. 177-182.
http://dx.doi.org/10.1002/jhm.864

[19]   K. E. Orr, M. G. Holliday, A. L. Jones, I. Robson and J. D. Perry, “Survival of Enterococci during Hospital Laundry Processing,” Journal of Hospital Infection, Vol. 50, No. 2, 2002, pp. 133-139.
http://dx.doi.org/10.1053/jhin.2001.1137

[20]   A. Hambraeus, S. Bengtsson and G. Laurell, “Bacterial Contamination in a Modern Operating Suite. 4. Bacterial Contamination of Clothes Worn in the Suite,” Journal of Hygiene (London), Vol. 80, No. 2, 1978, pp. 175-181.
http://dx.doi.org/10.1017/S0022172400053523

[21]   R. R. Banville and E. McNeil, “Microbiology of Drycleaning,” Applied Microbiology, Vol. 14, No. 1, 1966, pp. 1-7.

[22]   E. A. Hooker, S. Allen, L. Gray and C. Kaufman, “A Randomized Trial to Evaluate a Launderable Bed Protection System for Hospital Beds,” Antimicrobial Resistance and Infection Control, 2012.
http://dx.doi.org/10.1186/2047-2994-1-27

[23]   J. R. Babb, J. G. Davies and G. A. Ayliffe, “Contamination of Protective Clothing and Nurses' Uniforms in an Isolation Ward,” Journal of Hospital Infection, Vol. 4, No. 2, 1983, pp. 149-157.
http://dx.doi.org/10.1016/0195-6701(83)90044-0

[24]   G. Borkow and J. Gabbay, “Biocidal Textiles Can Help Fight Nosocomial Infections,” Medical Hypotheses, Vol. 70, No. 5, 2008, pp. 990-994.
http://dx.doi.org/10.1016/j.mehy.2007.08.025

[25]   G. M. L. Bearman, A. Rosato, K. Elam, et al., “A Crossover Trial of Antimicrobial Scrubs to Reduce MethicillinResistant Staphylococcus aureus Burden on Healthcare Worker Apparel,” Infection Control and Hospital Epidemiology, Vol. 33, No. 3, 2012, pp. 268-275.
http://dx.doi.org/10.1086/664045

 
 
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