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 AJAC  Vol.2 No.1 , February 2011
Rapid HPLC Method for Monitoring Relevant Residues of Pharmaceuticals Products in Environmental Samples
Abstract: This work presents a multi-residue analytical method based on solid phase extraction (SPE) followed by high-performance liquid chromatographic (HPLC) with diode array (DAD) detection for the simultaneous determination of a group of pharmaceutical products that include ten antidepressants and three anticanceri- genic in environmental samples (water and soil). Baseline separation of the studied compounds was obtained on an ultrabase C18 (4.6 mm i.d. × 150 mm, 5 μm particle) column using acetonitrile:phosphate buffer pH 2.5 (35:65 v/v) as mobile phase with a flow rate of 1.5 mL/min. Different aspects including linearity, accuracy, precision and detection and quantification limits were examined in order to validate the proposed method. Detection limits between 1 and 50 ng/mL were obtained for all the target compounds. This method was ap- plied to the analysis of environmental samples as waters and soils of different precedence. Prior, the HPLC determination the samples were purified and enriched using SPE or liquid-liquid extraction (LLE) of the tar- get compounds.
Cite this paper: nullJ. Flores, A. Salcedo and L. Fernández, "Rapid HPLC Method for Monitoring Relevant Residues of Pharmaceuticals Products in Environmental Samples," American Journal of Analytical Chemistry, Vol. 2 No. 1, 2011, pp. 18-26. doi: 10.4236/ajac.2011.21003.
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