Quantitative differentiation of phenolic compounds in different varieties of buckwheat cultivars from China, Japan and Korea

Jeong Min  Seo; Da Bin  Lee; Mariadhas  Valan Arasu; Qi  Wu; Tatsuro  Suzuki; Young-Ho  Yoon; Sang-Won  Lee; Sang Un  Park; Sun-Ju  Kim

doi:10.4236/jacen.2013.24016

JACEN Vol.2 No.4 , November 2013

Quantitative differentiation of phenolic compounds in different varieties of buckwheat cultivars from China, Japan and Korea

Jeong Min Seo, Da Bin Lee, Mariadhas Valan Arasu, Qi Wu, Tatsuro Suzuki, Young-Ho Yoon, Sang-Won Lee, Sang Un Park, Sun-Ju Kim^*

Abstract: The aim of this study was to determine the variation of phenolic compounds in common and tartary buckwheats collected from China, Japan and Korea. Two buckwheat varieties of each country were cultivated using nutrient solution and vermiculite in the greenhouse from February to May, 2012 and harvested at two-week intervals. Phenolics such as chlorogenic acid, C-glycosylflavones (orientin, isoorientin, vitexin, and isovitexin), rutin, and quercetin were quantified using high performance liquid chroma-tography (HPLC). At 17, 29, 44, 58, and 72 days after sowing (DAS), before harvesting each organs, rutin content of cultivars from China and Korea at 17 DAS was the highest (mean 43.63 and 39.95 mg?g–1 DW, respectively) than that of 29 and 44 DAS. However, two varieties from Japan at 44 DAS were documented maximum; whereas, the amount of rutin in “Hokkai T10” harvested at 44 DAS (58.36 mg?g–1 DW) was the highest of all cultivars. Rutin level in the leaves and stems at 72 DAS were comparatively higher than at 58 DAS. The highest of rutin content was found at 72 DAS (73.33 mg?g–1 DW) in “Hokkai T10” leaves followed by “Daegwan No.3-3” (61.13 mg?g–1 DW), “rice tartary” (53.89 mg?g–1 DW). Rutin content of flowers was presented as the highest amount in “Hokkai T10” at 72 DAS (88.3 mg?g–1 DW) was approximately 14-fold higher than that of 58 DAS (6.44 mg?g–1 DW). However, rutin content of flower in “Xiqiao No.2” was 3-fold higher at 72 DAS than at 58 DAS. Rutin and total phenolic compounds content in flowers, leaves, and stems were higher in the order. The content of four C-glycosylflavones in common buckwheat was higher than those in tartary buckwheat, but rutin content which accounted >90% of the total phenolic compounds was higher in tartary buckwheat. The highest amounts of chlorogenic acid and quercetin were measured in the flower of “Xiqiao No.2” (6.85 and 11.69 mg?g–1 DW, respectively). Based on these results, the presence of different phenolic compounds in all the varieties of buckwheat confirmed that it can be regarded as a potent source of functional foods.

Keywords: Buckwheat; Phenolic Compounds; Rutin; Functional Food

Cite this paper: J. Seo, D. Lee, M. Valan Arasu, Q. Wu, T. Suzuki, Y. Yoon, S. Lee, S. Park and S. Kim, "Quantitative differentiation of phenolic compounds in different varieties of buckwheat cultivars from China, Japan and Korea," Journal of Agricultural Chemistry and Environment, Vol. 2 No. 4, 2013, pp. 109-116. doi: 10.4236/jacen.2013.24016.

References

[1] Holasova, M., Fiedlerova, V., Smrcinova, H., Orsak, M., Lachman, J. and Vavreinova, S. (2002) Buckwheat—The source of antioxidant activity in functional foods. Food Research International, 35, 207-211. http://dx.doi.org/10.1016/S0963-9969(01)00185-5

[2] Kayashita, J., Shimaoka, I. and Nakajyoh, M. (1995) Hypocholes-terolemic effect of buckwheat protein extract in rats fed cholesterol enriched diets. Nutrition Research, 15, 691-698. http://dx.doi.org/10.1016/0271-5317(95)00036-I

[3] Tomotake, H., Shimaoka, I., Kayashita, J., Yokoyama, F., Nakajoh, M. and Kato, N.A. (2000) Buckwheat protein product suppresses gallstone formation and plasma cholesterol more strongly than soy protein isolate in hamsters. Journal of Nutrition, 130, 1670-1674.

[4] Aliaga, C. and Lissi, E.A. (2004) Comparison of the free radical scavenger activities of quercetin and rutin—An experimental and theoretical study. Canadian Journal of Chemistry, 82, 1668-1673. http://dx.doi.org/10.1139/v04-151

[5] Li, S.Q. and Zhang, Q.H. (2001) Advances in the development of functional foods from buckwheat. Critical Reviews in Food Science and Nutrition, 41, 451-464. http://dx.doi.org/10.1080/20014091091887

[6] Morishita, T., Yamaguchi, H. and Degi, K. (2007) The contribution of poly-phenols to antioxidative activity in common buckwheat and tartary buckwheat grain. Plant Production Science, 10, 99-104. http://dx.doi.org/10.1626/pps.10.99

[7] Fabjan, N., Rode, J., Kosir, I.J., Zang, Z. and Kreft, I. (2003) Tartary buckwheat (Fagopyrum tartaricum Gaertn.) as a source of dietary rutin and quercetrin. Journal of Agricultural and Food Chemistry, 51, 6452-6455. http://dx.doi.org/10.1021/jf034543e

[8] Liu, C.L., Chen, Y.S., Yang, J.H. and Chiang, B.H. (2008) Antioxidant activity of tartary (Fagopyrum tataricum (L.) Gaertn.) and common (Fagopyrum tataricum Gaertn). Buckwheat sprouts. Journal of Agricultural and Food Chemistry, 56, 173-178. http://dx.doi.org/10.1021/jf072347s

[9] Jackson, S.J. and Venema, R.C. (2006) Quercetin inhibits eNOS, microtubule polymerization, and mitotic progresssion in bovine aortic endothelial cells. Journal of Nutrition, 136, 1178-1184.

[10] Edwards, R.L., Lyon, T., Litwin, S.E., Rabovsky, A., Symons, J.D. and Jalili, T. (2007) Quercetin reduces blood pressure in hypertensive subjects. Journal of Nutrition, 137, 2405-2411.

[11] Kim S.J., Zaidul, I.S.M., Suzuki, T., Mukasa, Y., Hashimoto, N., Takigawa, S., Noda, T., Chie, M.E. and Yamauchi, H. (2008) Comparison of phenolic compositions between common and tartary buckwheat (Fag-opyrum) sprouts. Food Chemistry, 110, 814-820. http://dx.doi.org/10.1016/j.foodchem.2008.02.050

[12] Szostak, D.D. (2004) Flavonoids in hulls of different varieties of buckwheat and their antioxidant activity. Proceedings of the 9th International Symposium on Buckwheat, Prague, 18-22 August 2004, 621-625.

[13] Kim J.H., Lee B.C., Kim J.H., Sim G.S., Lee D.H., Lee K.E., Yun Y.P. and Pyo H.B. (2005) The isolation and antioxidative effects of vitexin from Acerpalmatum. Archives of Pharmacal Research, 28, 195-202. http://dx.doi.org/10.1007/BF02977715

[14] Devi P.U., Ganasaundari, A., Vrinda, B., Srinivasan, K.K. and Unnikrishnan, M.K. (2000) Radiation protection by the Ocimum flavonoids orientin and vicenin: Mechanism of action. Radiation Research, 154, 455-460. http://dx.doi.org/10.1667/0033-7587(2000)154[0455:RPBTOF]2.0.CO;2

[15] Amimoto, K., Yamazaki, A., Tokoro, K., Kudo, R. and Fukui, H. (1996) The ingredient analysis of vegetables for the purpose of chemical development. (The first report)-Comparison between the kinds of the lettuce ingredient (in Japanese). J-STAGE, 8, 146-153. http://dx.doi.org/10.2525/jshita.8.146

[16] Sharma, P. and Gujral, H.S. (2010) Antioxidant and polyphenol oxidase activity of germinated barley and its milling fractions. Food Chemistry, 120, 673-678. http://dx.doi.org/10.1016/j.foodchem.2009.10.059

[17] Tian, S., Nakamura, K. and Kayahara, H. (2004) Analysis of phenolic compounds in white rice, brown rice, and germinated brown rice. Journal of Agricultural and Food Chemistry, 52, 4808-4813. http://dx.doi.org/10.1021/jf049446f

[18] Cho S.Y., Lee Y.N. and Park, H.J. (2009) Optimization of ethanol extraction and further purification of isoflavones from soybean sprout cotyledon. Food Chemistry, 117, 312-317. http://dx.doi.org/10.1016/j.foodchem.2009.04.003

[19] Kim E.H., Kim S.H., Chung J.I., Chi H.Y., Kim J.A. and Chung, I.M. (2006) Analysis of phenolic compounds and isoflavones in soybean seeds (Glycine max (L.) Merill) and sprouts grown under different conditions. European Food Research and Technology, 222, 201-208. http://dx.doi.org/10.1007/s00217-005-0153-4

[20] Duenas, M., Hernandez, T., Estrella, I. and Fernandez, D. (2009) Germination as a process to increase the polyphenol content and antioxidant activity of lupin seeds (Lupinus angustifolius L.). Food Chemistry, 117, 599-607. http://dx.doi.org/10.1016/j.foodchem.2009.04.051

[21] Zielinski, H., Honke, J., ?atosz, A., Troszyńska, A., Ciska, E., Waszczuk, K., Szawara-Nowak, D. and Koz?owska, H. (1998) A rapid method for measurement of total antioxidant status of selected cereal grains-short report. Polish Journal of Food and Nutrition Sciences, 7, 533-538.

[22] Zielinski, H. and Troszyńska, A. (2000) Antioxidant capacity of raw and hydrothermal processed cereal grains. Polish Journal of Food and Nutrition Sciences, 9/50, 79- 83.

[23] Tahir, I. and Farooq, S. (1985) Grain composition in some buckwheat cultivars (Fagopyrum Spp.) with particular reference to protein fractions. Plant Foods for Human Nutrition, 35, 153-158. http://dx.doi.org/10.1007/BF01092131

[24] Oomah, B.D. and Mazza, G. (1996) Flavonoids and antioxidative activities in buckwheat. Journal of Agricultural and Food Chemistry, 44, 1746-1750. http://dx.doi.org/10.1021/jf9508357

[25] Park B.J., Park J.I., Chang K.J. and Park, C.H. (2004) Comparison in rutin content in seed and plant of tartary buckwheat (Fagopyrum tartaricum). Proceedings of the 9th International Symposium on Buckwheat, Prague, 18-22 August 2004, 626-629.