ENG  Vol.5 No.10 B , October 2013
Molecular Cloning, Characterization and Expression Analysis of Macrohage-Colony Stimulating Factor 2 Gene from Grass Carp (Ctenopharyngodon idellus)
Abstract

Macrophage colony-stimulating factor (CSF-1/M-CSF) is a key factor for the differentiation, growth and survival of monocytes/macrophages and osteoclasts. The functions of M-CSF have been well characterized in mammals. In this study, we have cloned and sequenced the cDNA of M-CSF2 in grass carp. The grass carp M-CSF2 cDNA was 1487 bp in length, containing an open reading frame of 855 bp that encodes 284 amino acids. The deduced protein of grass carp M-CSF2 possessed same domains similar to its mammalian counterparts. Multiple alignments and phylogenetic tree indicate that the grass carp M-CSF2 exhibits close evolutionary relationship with its counterparts in other teleosts. Lastly, the tissue distribution results also showed that the grass carp M-CSF2 transcript was dominantly expressed in head kidney, kidney and spleen in vivo.


Cite this paper
Du, L. , Qin, L. , Wang, S. , Yang, L. , Yang, K. , Wang, X. and Zhou, H. (2013) Molecular Cloning, Characterization and Expression Analysis of Macrohage-Colony Stimulating Factor 2 Gene from Grass Carp (Ctenopharyngodon idellus). Engineering, 5, 220-225. doi: 10.4236/eng.2013.510B046.
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