The dynamic speckle is a non-destructive optical technique that has been used as a tool for the characterization of the biological activity and several studies are conducted to obtain for more information about the correspondence of the observed phenomena and their expressions in the interference images. Analysis in the frequency domain has been considered as powerful alternative, and although there are works using Fourier transform in the frequency analysis of the biospeckle signals, the majority presents the wavelet transform as tool for spectral analysis. In turn, there are still doubts if the Fourier transform is not enough for the analysis of the biospeckle, which would enable the reduction of processing time since an operation is computationally simpler. In this context, the present study aims to compare the constituents’ parts of the speckle signal according to Fourier and wavelet transforms for numerical analysis. The comparative analysis based on the absolute values of the differences technique (AVD) was carried out for performance evaluation of the Fourier and wavelet transforms, in which the speckle signals were decomposed spectrally and subsequently reconstructed with the elimination of specific frequency bands. Results showed that the wavelet transform allowed more information about signals constituents of the dynamic speckle, emphasizing its use instead of the Fourier transform, which in turn was restricted the situations in which the only interest is to know the spectral content of the data.
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