ENG  Vol.5 No.10 B , October 2013
Bone Regeneration Enhanced by Antigen-Extracted Xenogeneic Cancellous Bone Graft with rhBMP-2 in Rabbits Mandibular Defect Repair
Abstract: The effects of large piece xenogeneic bone which was separated from healthy pigs as a scaffold on repair of mandibular defect was investigated and the applicability of antigen-extracted xenogeneic cancellous bone (AXCB) soaked with rhBMP-2 in bone defect repair was assessed. Mandibular defects were created in 48 New Zealand Rabbits, and then randomly divided into 4 groups, which was grafted in the mandibular defects with AXCB, AXCB soaked with rhBMP-2, autograft bone, or blank. Equal number of animals from each group was classified into three time points (4, 8, and 12 weeks) after operation for gross pathological observation, hematoxylin and eosin (H & E) staining, radiographic examination, and bone density measurement. H & E staining revealed that the area percentage of bone regeneration in the group of AXCB/rhBMP-2 graft was 27.72 ± 4.68, 53.90 ± 21.92, and 77.35 ± 9.83 when at 4, 8, and 12 weeks, which was better than that of auto bone graft, prompting that the group of AXCB/rhBMP-2 graft had commendable osteogenic effect. And comparing with the AXCB without rhBMP-2, of which the area percentage of bone regeneration was only 14.03 ± 5.02, 28.49 ± 11.35, and 53.90 ± 21.92, the osteogenic effect of AXCB/rhBMP-2 graft was demonstrated to be much better. In the group of AXCB/rhBMP-2 graft, the area percentage of bone regeneration increased, and the implanted materials were gradually degraded and replaced by autogenous bone regeneration over time. We concluded that antigen-extracted xenogeneic cancellous bone (AXCB) graft soaked with rhBMP-2 had shown excellent osteogenic effect in repair of bone defects, with good biocompability.
Cite this paper: Lai, R. , Li, Z. , Zhang, Y. and Zhou, Z. (2013) Bone Regeneration Enhanced by Antigen-Extracted Xenogeneic Cancellous Bone Graft with rhBMP-2 in Rabbits Mandibular Defect Repair. Engineering, 5, 108-113. doi: 10.4236/eng.2013.510B022.

[1]   C. Madrigal, R. Ortega, C. Meniz, et al., “Study of Available Bone Forinterforaminal Implant Treatment Using Cone-Beam Computed Tomography,” Medicina Oral Patologia Oral y Cirugia Bucal, Vol. 1, No. 13, 2008, pp. E307-312.

[2]   W. G. De Long Jr., T. A. Einhorn, K. Koval, et al., “Bone Grafts and Bone Graft Substitutes in Orthopaedic Trauma Surgery,” The Journal of Bone & Joint Surgery, Vol. 89, 2007, pp. 649-658.

[3]   E. N. Ebbesen, J. S. Thomsen and L. Mosekilde, “Nondestructive Determination of Iliac Crest Cancellous Bone Strength by pQCT,” Bone, Vol. 21, 1997, pp. 535-540.

[4]   C. G. Finkemeier, “Bone-Grafting and Bone-Graft Substitutes,” The Journal of Bone & Joint Surgery, Vol. 84A, 2002, pp. 454-464.

[5]   A. S. Herford and P. J. Boyne, “Reconstruction of Mandibular Continuity Defects with Bone Morphogenetic Protein-2 (rhBMP-2),” Journal of Oral and Maxillofacial Surgery, Vol. 66, 2008, pp. 616-624.

[6]   S. A. Jovanovic, D. R. Hunt, et al., “Bone reconstruction Following Implantation of rhBMP-2 and Guided Bone Re-generation in Canine Alveolar Ridge Defects,” Clinical Oral Implants Research, Vol. 18, 2007, pp. 224-230.

[7]   Z. Luo, Y. Hu and Q. Wang, “The Experimental Studies of Immune Response of Antigen-Extracted Bovine Cancellous Bone Grafting,” Zhonghua Wai Ke Za Zhi, Vol. 35, 1997, pp. 690-693.

[8]   S. Oeberg, C. Johansson and J. B. Rosenquist, “Bone Formation after Implantation of Autolysed Antigen Extracted Allogeneic Bone in Ovariectomized Rabbits,” International Journal of Oral and Maxillofacial Surgery, Vol. 32, 2003, pp. 628-632.

[9]   H. Schliephake, “Application of Bone Growth Factors: The Potential of Different Carrier Systems,” Oral and Maxil-lofacial Surgery, Vol. 14, 2010, pp. 17-22.

[10]   K. H. Schuckert, S. Jopp and S. H. Teoh, “Mandibular Defect Reconstruction Using Three-Dimensional Polyca-prolactone Scaffold in Combination with Platelet-Rich Plasma and Recombinant Human Bone Morphogenetic Protein-2: De Novo Synthesis of Bone in a Single Case,” Tissue Engineering Part A, Vol. 15, 2009, pp. 493-499.

[11]   C. Shi, W. Chen, Y. Zhao, et al., “Regeneration of Full-Thickness Abdominal Wall Defects in Rats Using Collagen Scaffolds Loaded with Collagen-Binding Basic Fibroblast Growth Factor,” Biomaterials, Vol. 32, 2011, pp. 753-759.

[12]   D. I. Spector, J. H. Keating and R. J. Boudrieau, “Immediate Mandibular Reconstruction of a 5 cm Defect Using rhBMP-2 after Partial Mandibulectomy in a Dog,” Veterinary Surgery, Vol. 36, 2007, pp. 752-759.

[13]   R. Visser, P. M. Arrabal, J. Becerra, et al., “The Effect of an rhBMP-2 Absorbable Collagen Sponge-Targeted System on Bone Formation in Vivo,” Biomaterials, Vol. 30, 2009, pp. 2032-2037.

[14]   B. Wenz, B. Oesch and M. Horst, “Analysis of the Risk of Transmitting Bovine Spongiform Encephalopathy through Bone Grafts Derived from Bovine Bone,” Biomaterials, Vol. 22, 2001, pp. 1599-1606.

[15]   J.-C. Xu, G.-H. Wu, H.-L. Liu, et al., “The Effect of Leptin on the Osteoinductive Activity of Recombinant Human Bone Morphogenetic Protein-2 in Nude Mice,” Saudi Medical Journal, Vol. 31, 2010, pp. 615-621.

[16]   M. Zhou, X. Peng, C. Mao, et al., “Primate Mandibular Reconstruction with Prefabricated, Vascularized Tissue-Engineered Bone Flaps and Recombinant Human Bone Morphogenetic Protein-2 Implanted in Situ,” Biomaterials, Vol. 31, 2010, pp. 4935-4943.