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 AJAC  Vol.4 No.10 A , October 2013
A Bioenergetic-Redox Approach to the Effect of Live Yeast on Ruminal pH during Induced Acidosis in Dairy Cow
Abstract: The objective of this study was to evaluate the capacity of the live yeast (LY) Saccharomyces cerevisiae in optimizing ruminal pH and in understanding its mode of action during induced acidosis in dairy cow. Two non-lactating cannulated cows were used and offered twice daily a control diet (CD) consisting of 51% corn silage and 49% concentrates or a LY diet (LYD) composed of CD supplemented with 4 g of LY per cow and per day. Measurements of pH and redox potential (Eh) were continuously made at 1 h interval during an experimental period of 9 h per day. Samples of ruminal fluid were also taken at 2 h intervals for analyses of volatile fatty acids (VFA) and lactic acid. Oxygen partial pressure (logPO2) in the ruminal milieu was calculated from the Nernst equation, using either O2-H2O or lactate-propionate redox couples. The results showed an increase of 0.2 unit in ruminal pH when LYD was fed, which was accompanied by a mean difference in Eh of -20 mV with respect to CD. The logPO2 decreased significantly by 0.8 log unit for LYD when compared to CD. Concentrations of VFA and proportion of propionate were higher with LYD (114.4 mM and 17.1% total VFA) compared to CD (102.4 mM and 15.4% total VFA). Proportion of butyrate decreased (from 15.8% to 14.2% total VFA). Lactate concentration decreased by 55% on average. It is proposed that the stabilization of ruminal pH (>6) is the outcome of the LY ability to scavenge oxygen. In doing so, it increased the reducing capacity of the milieu favoring the production of total VFA and depressing the level of lactate. The LY oxygen-scavenging ability was put forward to account for the transformation of lactate (pKa = 3.86) into propionate (pKa = 4.87) under reduced ruminal conditions.
Cite this paper: J. Marden, C. Bayourthe, E. Auclair and R. Moncoulon, "A Bioenergetic-Redox Approach to the Effect of Live Yeast on Ruminal pH during Induced Acidosis in Dairy Cow," American Journal of Analytical Chemistry, Vol. 4 No. 10, 2013, pp. 60-68. doi: 10.4236/ajac.2013.410A1008.
References

[1]   Z. Mir and P. S. Mir, “Effect of the Addition of Live Yeast (Saccharomyces cerevisiae) on Growth and Carcass Quality of Steers Fed High-Forage or High-Grain Diets and on Feed Digestibility and in Situ Degradability,” Journal of Animal Science, Vol. 72, No. 3, 1994, pp. 537-545.

[2]   P. F. Plata, G. D. Mendoza, J. R. Barcena-Gama and S. Gonzalez, “Effect of a Yeast Culture (Saccharomyces cerevisiae) on Neutral Detergent Fibre Digestion in Steers Fed Oat Straw Based Diets,” Animal Feed Science and Technology, Vol. 49, No. 3, 1994, pp. 203-210.
http://dx.doi.org/10.1016/0377-8401(94)90046-9

[3]   G. Piva, S. Belladonna, G. Fusconi and F. Sicbaldi, “Effects of Yeast on Dairy Cow Performance, Ruminal Fermentation, Blood Components, and Milk Manufacturing Properties,” Journal of Dairy Science, Vol. 76, No. 9, 1993, pp. 2717-2722.
http://dx.doi.org/10.3168/jds.S0022-0302(93)77608-0

[4]   D. A. Haimoud-Lekhal, P. Lescoat, C. Bayourthe and R. Moncoulon, “Effects of Saccharomyces cerevisiae and Aspergillus oryzae on Milk Yield and Composition in Dairy Cows: A Review,” Proceedings of the 6th Rencontres Recherches Ruminants, Paris, 1-2 December 1999, p. 157.

[5]   D. L. Swartz, L. D. Muller, G. W. Rogers and G. A. Varga, “Effect of Yeast Cultures on Performance of Lactating Dairy Cows: A Field Study,” Journal of Dairy Science, Vol. 77, No. 10, 1994, pp. 3073-3080.
http://dx.doi.org/10.3168/jds.S0022-0302(94)77249-0

[6]   P. H. Robinson and J. E. Garett, “Effect of Yeast Culture (Saccharomyces cerevisiae) on Adaptation of Cows to Post-Partum Diets on Lactational Performance,” Journal of Animal Science, Vol. 77, No. 4, 1999, pp. 988-999.

[7]   H. A. Lynch and S. A. Martin, “Effects of Saccharomyces cerevisiae Culture and Saccharomyces cerevisiae Live Cells on in Vitro Mixed Ruminal Microorganism Fermentation,” Journal of Dairy Science, Vol. 85, No. 10, 2002, pp. 2603-2608.
http://dx.doi.org/10.3168/jds.S0022-0302(02)74345-2

[8]   D. Girard and K. A. Dawson, “Effects of a Yeast Culture on the Growth Characteristics of Representative Ruminal Bacteria,” Journal of Animal Science, Vol. 72, Supplement I, 1994, p. 300.

[9]   S. A. Martin and D. J. Nisbet, “Effect of Direct-Fed Microbials on Ruminal Microbial Fermentation,” Journal of Dairy Science, Vol. 75, No. 6, 1992, pp. 1736-1744.
http://dx.doi.org/10.3168/jds.S0022-0302(92)77932-6

[10]   F. Chaucheyras-Durand, G. Fonty and G. Bertin, “Effect of a Strain of Saccharomyces cerevisiae (LEVUCELL SC), a Microbial Additive for Ruminants, on Lactate Metabolism in Vitro,” Canadian Journal of Microbiology, Vol. 42, No. 9, 1996, pp. 927-933.
http://dx.doi.org/10.1139/m96-119

[11]   P. E. V. Williams and C. J. Newbold, “Rumen Probiosis: The Effects of Novel Microorganisms on Rumen Fermentation and Ruminant productivity,” In: D. J. A. Cole and W. Haresign, Eds., Recent Advances in Animal Nutrition, Butterworths, London, 1990, pp. 211-227.

[12]   L. J. Erasmus, P. M. Botha and A. Kistner, “Effect of a Yeast Culture Supplement on Production, Rumen Fermentation and Duodenal Nitrogen Flow in Dairy Cows,” Journal of Dairy Science, Vol. 75, No. 11, 1992, pp. 3056-3065.
http://dx.doi.org/10.3168/jds.S0022-0302(92)78069-2

[13]   M. Doreau and J. P. Jouany, “Effect of Saccharomyces cerevisiae Culture on Nutrient Digestion in Lactating Dairy Cows,” Journal of Dairy Science, Vol. 81. No. 12, 1998. pp. 3214-3221.
http://dx.doi.org/10.3168/jds.S0022-0302(98)75885-0

[14]   R. J. Wallace, “Rumen Microbiology, Biotechnology, and Ruminant Nutrition: Progress and Problems,” Journal of Animal Science, Vol. 72, No. 11, 1994, pp. 2992-3003.

[15]   R. J. Wallace and C. J. Newbold, “Probiotics for Ruminants,” In: R. Fuller, Ed., Probiotics: The Scientific Basis, Chapman and Hall, London, 1992, pp. 317-353.

[16]   C. J. Newbold, R. J. Wallace and F. M. McIntosh, “The Stimulation of Rumen Bacteria by Saccharomyces cerevisiae is Dependent on the Respiratory Activity of the Yeast,” Journal of Animal Science, Vol. 71, Supplement 1, 1993, p. 280.

[17]   F. Mathieu, J. P. Jouany, J. Senaud, J. Bohatier, G. Bertin and M. Mercier, “The Effect of Saccharomyces cerevisiae and Aspergillus oryzae on Fermentations in the Rumen of Faunated and Defaunated Sheep: Protozoal and Probiotics Interactions,” Reproduction Nutrition Développement, Vol. 36, No. 3, 1996, pp. 271-287.
http://dx.doi.org/10.1051/rnd:19960305

[18]   M. Marounek, S. Bartos and G. I. Kalachnyuk, “Dynamics of the Redox Potential and rh of the Rumen Fluid of Goats,” Physiologia Bohemoslovaca, Vol. 31, No. 4, 1982, pp. 369-374.

[19]   C. J. Newbold, R. J. Wallace and F. M. McIntosh, “Mode of Action of the Yeast Saccharomyces cerevisiae as a Feed Additive for Ruminants,” British Journal of Nutrition, Vol. 76, No. 2, 1996, pp. 249-261.
http://dx.doi.org/10.1079/BJN19960029

[20]   R. I. Scott, N. Yarlett, K. Hillman, T. N. Williams, A. G. Williams and D. Lloyd, “The Presence of Oxygen in Rumen Liquor and Its Effects on Methanogenesis,” Journal of Applied Bacteriology, Vol. 55, No. 1, 1983, pp. 143-149.
http://dx.doi.org/10.1111/j.1365-2672.1983.tb02658.x

[21]   R. A. Kohn and R. C. Boston, “The Role of Thermodynamics in Controlling Rumen Metabolism,” In: J. P. Mc Namara, J. France and D. E. Beever, Eds., Modelling Nutrient Utilization in Farm Animals, CAB International, Wallingford, 2000, pp. 11-24.
http://dx.doi.org/10.1079/9780851994499.0011

[22]   J. P. Marden, C. Bayourthe, F. Enjalbert and R. Moncoulon, “A New Device for Measuring Kinetics of Ruminal pH and Redox Potential in Dairy Cow,” Journal of Dairy Science, Vol. 88, No. 1, 2005, pp. 277-281.
http://dx.doi.org/10.3168/jds.S0022-0302(05)72685-0

[23]   D. K. Nordstrom, “Thermochemical Redox Equilibria of Zo Bell’s Solution,” Geochimica and Cosmochimica Acta, Vol. 41, No. 12, 1977, pp. 1835-1841.
http://dx.doi.org/10.1016/0016-7037(77)90215-0

[24]   J. Vidal, “Thermodynamique: Application au Génie Chimique et à L’Industrie Pétrolière,” Editions OPHRYS., Paris, 1997.

[25]   V. T. Valsaraj, “Elements of Environmental Engineering: Thermodynamics and Kinetics,” 2nd Edition, Lewis Publishers, Chelsea, 2000, pp. 91-93.

[26]   T. Picek, M. Simek and H. Santruckova, “Microbial Responses to Fluctuation of Soil Aeration Status and Redox Conditions,” Biology and Fertility of Soils, Vol. 31, No. 3-4, 2000, pp. 315-322.
http://dx.doi.org/10.1007/s003740050662

[27]   H. C. Helgeson, C. E. Owens, A. M. Knox and L. Richard, “Calculation of the Standard Molal Thermodynamic Properties of Crystalline, Liquid and Gas Organic Molecules at High Temperatures and Pressures,” Geochimica and Cosmochimica Acta, Vol. 62, No. 6, 1998, pp. 985-1081.
http://dx.doi.org/10.1016/S0016-7037(97)00219-6

[28]   Y. Tardy, L. Mercury, C. Roquin and P. Vieillard, “Le Concept d’eau Ice-Like: Hydratation-Déshydratation des sels, Hydroxydes, Zéolites, Argiles et Matières Organiques Vivantes ou Inertes,” Comptes Rendus de l’Académie des Sciences—Series IIA—Earth and Planetary Science, Vol. 329, No. 6, 1999, pp. 377-388.

[29]   J. P. Jouany, “Volatile Fatty Acid and Alcohol Determination, in Digestive Contents, Silage Juices, Bacterial Cultures and Anaerobic Fermentor Contents,” Science des Aliments, Vol. 2, No. 2, 1982, pp. 131-144.

[30]   D. Sauvant, F. Meschy and D. Mertens, “Les Composantes de l’Acidose Ruminale et les Effets Acidogènes des Rations,” INRA Productions Animales, Vol. 12, No. 1, 1999, pp. 49-60.

[31]   L. J. Erasmus, P. M. Botha and A. Kistner, “Effect of a Yeast Culture Supplement on Production, Rumen Fermentation and Duodenal Nitrogen Flow in Dairy Cows,” Journal of Dairy Science, Vol. 75, No. 11, 1992, pp. 3056-3065.
http://dx.doi.org/10.3168/jds.S0022-0302(92)78069-2

[32]   L. O. Fiems, B. G. Cottyn, L. Dussert and J. M. Vanacker, “Effect of a Viable Yeast Culture on Digestibility and Rumen Fermentation in Sheep Fed Different Types of Diets,” Reproduction Nutrition Développement, Vol. 33, No. 1, 1993, pp. 43-49.
http://dx.doi.org/10.1051/rnd:19930104

[33]   K. A. Dawson, K. E. Newman and J. A. Boling, “Effects of Microbial Supplements Containing Yeast and Lactobacilli on Roughage Fed Microbial Activities,” Journal of Animal Science, Vol. 68, No. 10, 1990, pp. 3392-3398.

[34]   M. D. Carro, P. Lebzien and K. Rohr, “Influence of Yeast Culture on the in Vitro Fermentation (Rusitec) of Diets Containing Variable Portions of Concentrates,” Animal Feed Science and Technology, Vol. 37, No. 3-4, 1992, pp. 209-220.
http://dx.doi.org/10.1016/0377-8401(92)90005-Q

[35]   M. A. Cotta, “Interaction of Ruminal Bacteria in the Production and Utilisation of Malto-Oligosaccharides from Starch,” Applied and Environmental Microbiology, Vol. 58, No. 1, 1992, pp. 48-54.

[36]   G. H. M. Counotte, R. A. Prins., R. A. M. Janssen and J. A. de Biem, “Role of Megasphera elsdenii in the Fermentation of Dl-(2-13C) Lactate in the Rumen of Dairy Cattle,” Applied and Environmental Microbiology, Vol. 42, No. 4, 1981, pp. 649-655.

[37]   J. B. Russel and R. B. Hespell, “Microbial Rumen Fermentation,” Journal of Dairy Science, Vol. 64, No. 6, 1981, pp. 1153-1169.
http://dx.doi.org/10.3168/jds.S0022-0302(81)82694-X

[38]   G. Broberg, “Oxygen’s Significance for the Ruminal Flora as Illustrated by Measuring the Redox Potential in Rumen Contents,” Nordisk Veterinaermedicin, Vol. 9. 1957, pp. 57-60.

[39]   G. Broberg, “Measurements of the Redox Potential in Rumen Contents. III. Investigations into the Effect of Oxygen on the Redox Potential and Quantitative in Vitro Determinations of the Capacity of Rumen Contents to Consume Oxygen,” Nordisk Veterinaermedicin, Vol. 9, 1957, pp. 942-950.

[40]   T. N. Barry, A. Thompson and D. G. Armstrong, “Rumen Fermentation Studies on Two Contrasting Diets. 1. Some Characteristics of the in Vivo Fermentation, with Special Reference to the Composition of the Gas Phase, Oxidation/Reduction State and Volatile Fatty Acid Proportions,” Journal of Agricultural Science, Vol. 89, No. 1, 1977, pp. 183-195.
http://dx.doi.org/10.1017/S0021859600027362

[41]   P. V. D. Andrade, S. Giger-Reverdin and D. Sauvant, “Relation Entre Deux Paramètres (pH et Potentiel Redox) Caractérisant l’état du Rumen. Effet de la Nature de la Ration,” Proceedings of the 9th Rencontres Recherches Ruminants, Paris, 4-5 December 2002, p. 9.

[42]   L. P. Broudiscou, A. Agbabla-Dohnani, Y. Papon, A. Cornu, E. Grenet and A. Broudiscou, “Quantitative Effects of Alfalfa Extract Supply on Rice Straw Degradation, Fermentation and Biomass Synthesis by Rumen Microorganisms in Vitro,” Animal Research, Vol. 50, No. 6, 2001, pp. 429-440.
http://dx.doi.org/10.1051/animres:2001105

[43]   B. Gedek, C. Enders, F. Ahrens and C. Roques, “The Effect of Saccharomyces cerevisiae (BIOSAF Sc 47) on Ruminal Flora and Rumen Fermentation Pattern in Dairy Cows,” Annales de Zootechnie, Vol. 42, No. 2, 1993, p. 175. http://dx.doi.org/10.1051/animres:19930243

[44]   I. Chademana and N. W. Offer, “The Effect of Dietary Inclusion of Yeast Culture on Digestion in the Sheep,” Animal Production, Vol. 50, No. 3, 1990, pp. 483-489.
http://dx.doi.org/10.1017/S0003356100004967

[45]   G. A. harrison, R. W. Hemken and K. A. Dawson, “Influence of Addition of Yeast Culture Supplement to Diets of Lactating Cows on Ruminal Fermentation and Microbial Populations,” Journal of Dairy Science, Vol. 71, No. 11, 1988, pp. 2967-2975.
http://dx.doi.org/10.3168/jds.S0022-0302(88)79894-X

[46]   D. J. Nisbet and S. A. Martin, “Effect of Saccharomyces cerevisiae Culture on Lactate Utilization by the Ruminal Bacterium Selenomonas ruminantium,” Journal of Animal Science, Vol. 69, No. 11, 1991, pp. 4628-4633.

[47]   F. Chaucheyras-Durand, G. Fonty and G. Bertin, “L’Utilisation des Levures Vivantes, Additif Microbien chez le Ruminant,” Bulletin des Groupements Techniques Vétérinaires, Vol. 576, No. 5B, 1997, pp. 32-52.

[48]   J. D. Cox, D. D. Wagman and V. A. Medvedev, “CO-DATA Key Values for Thermodynamics,” Hemisphere Publishing Corporation, New York, 1989.

 
 
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