CWEEE  Vol.2 No.3 B , July 2013
Silver Nanoparticle Adsorption to Soil and Water Treatment Residuals and Impact on Zebrafish in a Lab-scale Constructed Wetland
ABSTRACT

Nanoparticles (< 100 nm) are becoming more prevalent in residential and industrial uses and may enter the environment through wastewater. Although lab studies have shown that nanoparticles can be toxic to various organisms, limited research has been done on the effects of nanoparticles in the environment. Environmental conditions such as pH and ionic strength are known to alter the biotoxicity of nanoparticles, but these effects are not well understood. The objectives of this research were to determine the impacts of silver nanoparticles (AgNP) on zebrafish in the pseudo-natural environment of a lab-scale constructed wetland, and to investigate wastewater remediation through soil and water treatment residual (WTR) adsorption of AgNPs. Concurrently, the effect of particle size on AgNP sorption was examined. Researchers exposed adult zebrafish in a lab-scale constructed wetland to concentrations of AgNP ranging from 0 - 50 mg AgNP/L and compared them to negative controls with no silver exposure and to positive controls with exposure to silver nitrate. The results suggest that aggregated AgNP do not impact zebrafish. Separately, sorption experiments were carried out examining three media - a wetland soil, a silt loam soil, and a WTR - in their capacity to remove AgNPs from water. The silt loam retained less AgNPs from solution than did the wetland soil or the WTR. In the WTR AgNPs were associated with sand size particles (2 mm - 0.05 mm), but in the wetland soil and silt loam, approximately half of the AgNPs were associated with the sand-sized particles, while the rest were associated with silt sized (~0.05 mm) or smaller particles. The larger sorption capacity of the wetland soil and WTR was attributed to their higher carbon content. The sorption data indicate that AgNPs adsorbed to soil and WTRs and support the idea that natural and constructed wetlands can remove AgNPs from wastewater.


Cite this paper
Ebeling, A. , Hartmann, V. , Rockman, A. , Armstrong, A. , Balza, R. , Erbe, J. and Ebeling, D. (2013) Silver Nanoparticle Adsorption to Soil and Water Treatment Residuals and Impact on Zebrafish in a Lab-scale Constructed Wetland. Computational Water, Energy, and Environmental Engineering, 2, 16-25. doi: 10.4236/cweee.2013.23B004.
References
[1]   WHO: World Health Organization, “Small-scale Water Supplies in the pan-European Region,” United Nations Economic Commission for Europe, 2010.

[2]   T. Brick, B. Primrose, R. Chandrasekhar, S. Roy, J. Muliyil, G. Kang, “Water Contamination in Urban South India: Household Storage Practices and Their Implications for Water Safety and Enteric Infections,” Journal of Hygiene and Environmental Health, Vol. 207, No. 5, 2004, pp. 473-480.doi:10.1078/1438-4639-00318

[3]   R. B. Levin, P. R. Epstein, T. E. Ford, W. H. Harrington, E. Olson, E. G. Reichard, “U.S. Drinking Water Challenges in the Twenty-First Century,” Environmental Health Perspectives, Vol. 100, 2002, pp. 43-52. doi:10.1289/ehp.02110s143

[4]   A. J. Jowet, “China’s Water Crisis,” The Geographical Journal, Vol. 152, No. 1, 1986, pp. 9-18. doi:10.2307/632934

[5]   P. Vonlanthen, D. Bittner, A. G. Hudson, K. A. Young, R. Muller, B. Lundsgaard-Hansen, D. Roy, S. Di Piazza, C. R. Largiader and O. Seehausen, “Eutrophication Causes Speciation Reversal in Whitefish Adaptive Radiations”, Nature, Vol. 482, 2012, pp. 357-362. doi:10.1038/nature10824

[6]   S. S. Kaushal, W. M. Lewis Jr., and J. H. McCutehan Jr., “Land Use Change and Nitrogen Enrichment of a Rocky Mountain Watershed,” Ecological Applications, Vol. 16, No. 1, 2006, pp. 299-312. doi:10.1890/05-0134

[7]   J. Fawell and M. J. Nieuwen-huijsen, “Contaminants in Drinking Water,” British Medical Bulletin, Vol. 68, No. 1, 2003, pp. 199-208. doi:10.1093/bmb/ldg027

[8]   S. N. Levine and D. W. Schindler, “Influence of Nitrogen to Phosphorus Supply Ratios and Physicochemical Conditions on Cyanobacteria and Phytoplankton Species Composition in the Experimental Lakes Area, Canada,” Canadian Journal of Fisheries and Aquatic Sciences, Vol. 56, No. 3, 1999, pp. 451-466. doi:10.1139/f98-183

[9]   D. L. Correll, “The Role of Phosphorus in the Eutrophication for Receiving Waters: A Review,” Journal of Environmental Quality, Vol. 27, No. 2, 1998, pp. 261-266. doi:10.2134/jeq1998.00472425002700020004x

[10]   T. C. Daniel, A. N. Sharpley, and J. L. Lumunyon, “Agricultural Phosphorus and Eutrophication: A Symposium Overview,” Journal of Environmental Quality, Vol. 27, No. 2, 1998, pp. 251-257. doi:10.2134/jeq1998.00472425002700020002x

[11]   E. G. Srinath and S. C. Pillai, “Phosphorus in Sewage, Polluted Waters, Sludges, and Effluents,” The Quarterly Review of Biology, Vol. 41. No. 4, 1966, pp. 384-407. doi:10.1086/405158

[12]   C. N. Sawyer, H. B. Gotaas, and J. B. Lackey, “Factors Involved in Disposal of Sewage Effluents to Lakes,” Sewage and Industrial Wastes, Vol. 26, No. 3, 1954, pp. 317-328.

[13]   S. K. Liehr, “Natural Treatment and Onsite Processes,” Water Environment Research, Vol. 77, No. 6, 2005, pp. 1389-1424. doi:10.2175/106143005X54416

[14]   UN, “Waste-Water Treatment Technologies: A General Review,” Economic and Social Commission for Western Asia, New York, 2003.

[15]   U. Mander and P. D. Jenssen Eds., “Constructed Wetlands for Waste Water Treatment in Cold Climates,” WIT Press, Southampton, 2002.

[16]   E. S. Bernhardt, B. P. Colman, M. F. Hochella, Jr., B. J. Cardinale, R. M. Nisbet, C. J. Richardson, and L. Yin, “An Ecological Perspective on Nanomaterial Impacts in the Environment,” Journal of Environmental Quality, Vol. 39, No. 6, 2010, pp. 1-12. doi:10.2134/jeq2009.0479

[17]   A. B. A. Boxall, M. A. Rudd, B. W. Brooks, D. J. Caldwell, K. Choi, S. Hickmann, E. Innes, K. Ostapyk, J. P. Staveley, T. Verslycke, G. T. Ankley, K. F. Beazley, S. E. Benlanger, J. P. berninger, P. Carriquiriborde, A. Coors, P. DeLeo, S. D. Dyer, J. F. Ericson, J. Gagne, J. P. Biesy, T. Gouin, L. Hallstrom, M. V. Karlsson and D. G. J. Larsson, “Pharmaceuticals and Personal Care Products in the Environment: What Are the Big Questions?” Environmental Health Perspectives, Vol. 120, No. 9, 2012, pp. 1221-1229. doi:10.1289/ehp.1104477

[18]   S. Rodrigues-Mozaz and H. S. Weinberg, “Meeting Report: Pharmaceuticals in Water–An Interdisciplinary Approach to a Public Health Challenge,” Environmental Health Perspectives, Vol. 118, No. 7, 2010, pp. 1016-1020. doi:10.1289/ehp.0901532

[19]   J. Fabrega, S. N. Luoma, C. R. Tyler, T. S. Galloway and J. R. Lead, “Silver Nano-particles: Behaviour and Effects in the Aquatic Environment,” Environmental International, Vol. 37, No. 2, 2011, pp.517-531. doi:10.1016/j.envint.2010.10.012

[20]   A. M. Comerton, R. C. Andrews and D. M. Bagley, “Practical Overview of Analytical Methods for Endocrine-Disrupting Compounds, Pharmaceuticals, and Personal Care Products in Water and Wastewater,” Philisophical Transacations: Mathematical, Physical, and Engineering Sciences, Vol. 367, No. 1904, 2009, pp. 3923-3939.

[21]   C. G. Daugh-ton and T. A. Ternes, “Pharmaceuticals and Personal Care Products in the Environment: Agents of Subtle Change?” Environmental Health Perspectives, Vol. 107, 1999, pp. 907-938. doi:10.1289/ehp.99107s6907

[22]   N. B. Golovina and L. M. Kustov, “Toxicity of Metal Nanoparticles with a Focus on Silver,” Mendeleev Communications, Vol. 23, No. 2, 2013, pp. 59-65. doi:10.1016/j.mencom.2013.03.001

[23]   C. You, C. Han, X. Wang, Y. Zheng, Q. Li, X. Hu and H. Sun, “The Progress of Silver Nanoparticles in the Antibacterial Mechanism, Clinical Application, and Cytotoxicity,” Molecular Biology Reports, Vol. 39, No. 9, 2012, pp. 9093-9201. doi:10.1007/s11033-012-1792-8

[24]   K. Kulthong, S. Srising, K. Boonpavanitchak, W. Kangwansupamonkon and R. Maniratanachote, “Determination of Silver Nanoparticle Release from Antibacterial Fabrics into Artificial Sweat,” Particle Fibre Toxicology, Vol. 7, 2010, pp. 8. doi:10.1186/1743-8977-7-8

[25]   B. Karn, T. Kuiken and M. Otto, “Nanotechnology and in Situ Remediation: A Review of the Benefits and Potential Risks,” Environmental Health Perspectives, Vol. 117, No. 12, 2009, pp. 1823-1831.

[26]   M. Yousefian and B. Payam, “Effects of Nanochemical Particles on Some Histological Parameters of Fish,” Advances in Environmental Biology, Vol. 6, No. 3, 2012, pp. 1209-1215.

[27]   F. Gagne, C. Andre, R. Skirrow, M. Gelinas, J. Auclair, G. van Aggelen, P. Turcotte and C. Gagnon, “Toxicity of Silvernanparticles to Rainbow Trout: a Toxicogenomic Approach,” Chemosphere, Vol. 89, No. 5, 2012, pp. 615-622. doi:10.1016/j.chemosphere.2012.05.063

[28]   S. W. Kim and Y. J. An, “Effect of ZnO and TiO2 Nanoparticles Preilluminated with UVA and UVB light on Escherichia coli and Bacillus subtilis,” Applied Microbiology and Biotechnology, Vol. 95, No. 1, 2012, pp. 243-253.doi:10.1007/s00253-012-4153-6

[29]   D. A. Cowart, S. M. Guida, S. Ismat and A. G. Marsh, “Effects of Ag Nanoparticles on Survival and Oxygen Consumption of Zebrafish Embryos, Danio rerio,” Journal of Environmental Science and Health, Part A: Toxic/Hazardous Substances and Environmental Engineering, Vol. 46, No. 10, 2011, pp. 1122-1128.

[30]   D. Stampoulis, S. K. Sinha and J. White, “Assay-Dependent Phytotoxicity of Nanoparticles to Plants,” Environmental Science and Technology, Vol. 43, No. 24, 2009, pp. 9473-9479. doi:10.1021/es901695c

[31]   R. J. Griffit, J. Luo, J. Gao, J. C. Bonzongo and D. S. Barber, “Effects of Particle Composition and Species on Toxicity of Metallic Nano-materials in Aquatic Organisms,” Nanomaterials in the Environment, Vol. 27, No. 9, 2008, pp. 1972-1978. doi:10.1016/j.scitotenv.2006.11.007

[32]   K. Y. Yoon, J. H. Byeon, J. H. Park and J. H. wang, “Susceptibility Constants of Escherichia coli and Bacillus subtilis to Silver and Copper Nanoparticles,” Science of the Total Environment, Vol. 373, No. 2-3, 2007, pp. 572-575.doi:10.1016/j.scitotenv.2006.11.007

[33]   A. S. Barnard and H. Guo, “Nature’s Nanostructures,” Pan Stanford Publishing, Singapore, 2012.

[34]   N. J. Kagengi and A. Thompson, “The Emerging Emphasis on Nanometer-Scale Processes in Soil Environments,” Soil Science Society of America Journal, Vol. 75, No. 2, 2011, pp. 333-334. doi:10.2136/sssaj2011.000npsintro

[35]   B. K. G. Theng and G. Yuan, “Nanoparticles in the Soil Environment,” Elements, Vol. 4, No. 6, 2008, pp. 395-399. doi:10.2113/gselements.4.6.395

[36]   J. M. Zook, M. D. Halter, D. Cleveland and S. E. Long, “Disentangling the Effects of Polymer Coatings on Silver Nanoparticle Agglomeration, Dissolution, and Toxicity to Determine Mechanisms of Nanotoxicity,” Journal of Nanoparticle Research, Vol. 14, 2012, pp. 1165-1572 doi:10.1007/s11051-012-1165-1

[37]   K. R. Reddy, E. M. D’Angelo and W. G. Harris, “Biochemistry of Wetlands,” In Handbook of Soil Science, M.E. Sumner (Ed.), CRC Press, 1999.

[38]   J. A. Ippolito, K. A. Barbarick and H. A. Elliott, “Drinking Water Treatment Residuals: A Review of Recent Uses,” Journal of Environmental Quality, Vol. 40, No.1, 2011, pp. 1-12. doi:10.2134/jeq2010.0242

[39]   E. A. Dayton and N. T. Basta, “A Method for Determining the Phosphorus Sorption Capacity and Amorphous Aluminum of Aluminum-Based Drinking Water Treatment Residuals,” Journal of Environmental Quality, Vol. 34, No.3, 2005, pp. 1112-1118.doi:10.2134/jeq2004.0230

[40]   P. S. Nair, T. J. Logan, A. N. Sharpley, L. E. Sommers, M. A. Tabatabai and T. L. Yuan, “Interlaboratory Comparison of a Standardized Phosphorus Adsorption Procedure,” Journal of Environmental Quality, Vol. 13, No. 4, 1984, pp. 591-595. doi:10.2134/jeq1984.00472425001300040016x

[41]   H. A. Elliot, G. A. O’Connor, P. Lu and S. Brinton, “Influence of Water Treatment Residuals on Phosphorus Solubility and Leaching,” Journal of Environmental Quality, Vol. 31, 2002, pp. 1362-0.69.

[42]   G. J. Bouyoucos, “Hydrometer Method Improved for Making Particle Size Analysis of Soils,” Agronomy Journal, Vol. 54, No. 5, 1962, pp. 464-465. doi:10.2134/agronj1962.00021962005400050028x

[43]   G. W. Gee and J. W. Bauder, “Particle-size Analysis,” In A. Klute, Ed., Methods of soil analysis. Part 1. 2nd ed. Agronomy Monograph 9. ASA and SSSA, Madison, WI, 1986, pp. 383-411.

[44]   T. M. Benn and P. Westerhoff, “Nanoparticle Silver Released into Water from Commercially Available Sock Fabrics,” Environmental Science and Technology, Vol. 42, No. 11, 2008, pp. 4133-4139. doi;10.1021/es7032718

[45]   EPA. “Method 3050B (SW-846): Acid Digestion of Sediments, Sludges, and Soils,” Revision 2, 1996. http://www.epa.gov/sam/pdfs/EPA-3050b.pdf

[46]   J. Gao, K. Powers, Y. Wang, H. Zhou, S.M. Roberts, B. M. Moudgil, B. Kooopman and D. S. Barber, “Influence of Suwannee River Humic Acid on Particle Properties and Toxicity of Silver Nanoparticles,” Chemosphere, Vol. 89, No. 1, 2012 pp. 96-101. doi:10.1016/j.chemosphere.2012.04.024

[47]   F. Gottschalk, E. Kost and B. Nowack, “Engineered Nanomaterials in Waters and Soils: A Risk Quantification Based on Probabilistic Exposure and Effect Modeling,” Environmental Toxicology and Chemistry, 2013, (In Press). doi:10.1002/etc.2177

[48]   B. C. Reinsch, C. Levard, Z. Li, R. Ma, A. Wise, K. B. Gregory, G. E. J. Brown and G. V. Lowry, “Sulfidation of Silver Nanoparticles Decreases Escherichia coli Growth Inhibition,” Environmental Science and Technology, Vol. 46, No. 13, 2012, pp. 6992-7000.doi:10.1021/es203732x

[49]   R. Kaegi, A. Boegelin, B. Sinnet, S. Zuleeg, H. Hagendorfer, M. Berkhardt and H. Siegrist, “Behavior of Metallic Silver Nanoparticles in a Pilot Wastewater Treatment Plant,” Environmental Science and Technology, Vol. 45, No. 9, 2011, pp. 3902-3908. doi:10.1021/es1041892

[50]   H. T. Ratte, “Bioaccumulation and Toxicity of Silver Compounds: A Review,” Environmental Toxicology and Chemistry, Vol. 18, No. 2, 1999, pp. 89-108. doi:10.1002/etc.5620180112

[51]   Lenntech BV, “Detergents Occurring in Freshwater,” 2012 http://www.lenntech.com/aquatic/detergents.htm.

[52]   G. E. Batley, J. K. Kirby, M. J. McLaughlin, “Fate and Risks of Nanomaterials in Aquatic and Terrestrial Environments,” Accounts of Chemical Research, Vol. 46, No. 3, 2013, pp. 854-862.doi:10.1021/ar2003368

[53]   J. M. Unrine, B .P. Colman, A. J. Bone, A. P. Gondikas and C. W. Matson, “Biotic and Abiotic Interactions in Aquatic Microcosms Determine Fate and Toxicity of Ag Nanoparticles. Part 1. Aggregation and Dissolution,” Environmental Science and Technology, Vol. 46, No.13, 2012, pp. 6915-6924. doi:10.1021/es204682q

[54]   P. Zhang, X. He, Y. Ma, K. Lu, Y. Zhao and Z. Zhang, “Distribution and Bioavailability of Ceria Nanoparticles in an Aquatic Ecosystem Model,” Chemosphere, Vol. 89, No. 5, 2012, pp. 530-535. doi:10.1016/j.chemosphere.2012.05.044

[55]   A. M. Badawy, T. P. Luxton, R. G. Silva, K. G. Scheckel, M. T. Suidan and T. M. Tolaymat, “Impact of Environmental Conditions (pH, Ionic Strength, and Electronlyte Type) on the Surface Charge and Aggregation of Silver Nanoparticles Suspensions,” Environmental Science and Technology, Vol. 44, No. 4, 2010, pp. 1260-1266. doi:10.1021/es902240k

[56]   W. A. Shoults-Wilson, B. C. Reinsch, O. V. Tysusko, P. M. Bertsch, G. V. Lowry and J. M. Unrine, “Role of Particle Size and Soil Type in Toxicity of Silver Nanoparticles to Earthworms,” Soil Science Society of America Journal, Vol. 75, No. 2, 2011, pp. 365-377. doi:10.2136/sssaj2010.0127nps

[57]   D. Cleveland, S. E. Long, P. L. Pennington, E. Cooper, M. Fulton, G. I. Scott, T. Brewer, J. Davis, E. J. Petersen and L. Wood, “Pilot Estuarine Mesocosm Study on the Environmental Fate of Silver Nanomaterials Leached from Consumer Products,” Science of the Total Environment, Vol. 421-422, No. 5, 2012, pp. 267-272. doi:10.1002/ieam.5630030316

[58]   R. D. Handy and B. J. Shaw, “Ecotoxicity of Nanomaterials to Fish: Challenges for Ecotoxicity Testing,” Integrated Environmental Assessment and Management, Vol. 3, No. 3, 2007, pp. 458-460. doi:10.1002/ieam.5630030316

 
 
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