JSEMAT  Vol.3 No.4 A , October 2013
AFM Investigation of the Organization of Actin Bundles Formed by Actin-Binding Proteins
Abstract: AFM is a powerful technique for revealing the morphological features of various biological systems at high resolution. However, one of the complications of AFM is that samples must be attached to a flat surface in order to obtain images. This often requires the development of specialized methods depending on the sample which is being used. In this study, we developed a novel technique to image actin bundles on the mica surface. Using this technique, we were able to image molecular assemblies of F-actin with two actin remodeling proteins: α-actinin and Caprice. High resolution AFM images of F-actin fibers and bundle organization depicted two different types of molecular assemblies: F-actin bundles forming an elongated “zipper” structure in the presence of α-actinin, and bundles forming a perpendicularly crossing the mesh structure in the presence of Caprice.
Cite this paper: Gilmore, J. , Kumeta, M. and Takeyasu, K. (2013) AFM Investigation of the Organization of Actin Bundles Formed by Actin-Binding Proteins. Journal of Surface Engineered Materials and Advanced Technology, 3, 13-19. doi: 10.4236/jsemat.2013.34A1002.

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