JSEMAT  Vol.3 No.4 A , October 2013
AFM Investigation of the Organization of Actin Bundles Formed by Actin-Binding Proteins
ABSTRACT

AFM is a powerful technique for revealing the morphological features of various biological systems at high resolution. However, one of the complications of AFM is that samples must be attached to a flat surface in order to obtain images. This often requires the development of specialized methods depending on the sample which is being used. In this study, we developed a novel technique to image actin bundles on the mica surface. Using this technique, we were able to image molecular assemblies of F-actin with two actin remodeling proteins: α-actinin and Caprice. High resolution AFM images of F-actin fibers and bundle organization depicted two different types of molecular assemblies: F-actin bundles forming an elongated “zipper” structure in the presence of α-actinin, and bundles forming a perpendicularly crossing the mesh structure in the presence of Caprice.


Cite this paper
J. Gilmore, M. Kumeta and K. Takeyasu, "AFM Investigation of the Organization of Actin Bundles Formed by Actin-Binding Proteins," Journal of Surface Engineered Materials and Advanced Technology, Vol. 3 No. 4, 2013, pp. 13-19. doi: 10.4236/jsemat.2013.34A1002.
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