Podophyllum hexandrum Royle is an important medicinal plant that
produces podophyllotoxin with anti-cancer properties. In China, it is used as a
traditional Chinese medicine. Recent years, unplanned exploiting and collection
have led to the disappearance of this species in China and India. Effective
methods such as tissue culture should be adopted to conserve it to abtain a
large scale. It was an crucial process for the final success of tissue culture
that seedlings with roots and true leaves in flasks were transferred to soil in
field. A protocol has been development for in vitro rooting and hardening
and en vitro transplant of Podophyllum
hexandrum Royle. Roots were efficiently established on WPM media
supplemented with IAA 1.5 mg·L-1 and NAA 0.5 mg·L-1.
Before transferred to soil containing turfysoil and perlite (2:1), rooted
plants were exposed to air for 5d for adaptation. Two periods were good for
transplanting this plantlets.
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