ENG  Vol.4 No.10 , October 2012
The Poly(vinyl alcohol)-Immobilized Photobacteria for Toxicology Monitoring
Abstract
The present work is undertaken to studies the important factors affecting the stability of a light emission at immobilized photobacteria, and application PVAGs luminous biosensors for biomonitoring of toxicants. The intensity and stability of a light emission is competently controlled by: 1) intensity and persistens of a luminescent cycle using bacterial strain; 2) type of the carrier and the composition of the gel-formation medium; 3) freeze-thawing procedures; 4) physical and chemical conditions of storage and application.

Cite this paper
K. Alenina, L. Aleskerova, P. Kascheyeva and A. Ismailov, "The Poly(vinyl alcohol)-Immobilized Photobacteria for Toxicology Monitoring," Engineering, Vol. 4 No. 10, 2012, pp. 118-120. doi: 10.4236/eng.2012.410B030.
References

[1]   Bulich AA (1979) Use of luminescent bacteria for determining toxicity in aquatic environments. In: Markings LL, Kimerle RA (eds.) Aquatic Toxicology. American Society for Testing and Materials, Philadelphia

[2]   Lee JH, Mitchell RJ, Kim BC, Cullen DC, Gu MB (2005) A cell array biosensor for environmental toxicity analysis. Biosens Bioelectron 21(3):500–507

[3]   Scheller F, Schubert F (1992) Biosensors. Techniques and instrumentation in analytical chemistry. Elsevier, Amsterdam 11:359

[4]   Park KS, Baumstark-Khan Ch, Rettberg P, Horneck G, Rabbow E, Gu MB (2005) Immobilisation as a technical possibility for long-term storage of bacterial biosensors. Radiat Environ Biophys 44:69–71

[5]   Lee B, Lee J, Shin D, Kim E (2006) Statistical optimization of bioluminescence Photobacterium phosphoreum KCTC 2852. Environ Int 32(2):265–268

[6]   Lozinsky VI, Zubov AL, Titova EF (1996) Swelling behaviour of poly(vinyl alcohol) cryogels employed as matrices for cell immobilization. Enzyme Microb Technol 18(6):561-569

[7]   Lozinsky VI, Plieva FM (1998) PoIy(vinyl alcohol) cryogels employed as matrices for cell immobilization. 3. Overview of recent research and developments. Enzyme Microb Technol 23:227–242

[8]   Chun UH, Simonov N, Chen Y, Britzb ML (1996) Continuous pollution monitoring using Photobacterium phosphoreum. Resour Conserv Recycl 18:25–40

[9]   Efremenko EN, Senko OV, Kuts VV, Alenina KA, Kholstov AV, Ismailov AD (2010) The luminescent biocatalyst for detecting toxicants. Patent RU 2394910

[10]   Hastings JW, Weber G (1963) Total quantum flux of isotropic sources. Opt Soc Am 53(12):1410–1415

[11]   Efremenko EN, Tatarinova NYu (2007) The effect of long-term preservation of bacterial cells immobilized in poly(vinyl alcohol) cryogel on their viability and biosynthesis of target metabolites. Microbiol 76(3):383–389

 
 
Top