ABB  Vol.4 No.10 , October 2013
Effects of light intensities and photoperiods on growth and proteolytic activity in purple non-sulfur marine bacterium, Afifella marina strain ME (KC205142)
Abstract: Afifella marina strain ME (KC205142), a purple non-sulfur bacterium was isolated from mangrove habitats of Sabah. The effects of light intensities and photoperiods on proteolytic activity in Afifella marina strain ME (KC205142) were investigated. Secretion of proteolytic enzymes in Afifella marina was preliminarily assessed by skim milk agarose media. Subsequently, light intensities, such as, dark, 1000, 1500, 2000, 2500, 3000, 3500, 4000, 4500 and 5000 lux were used to evaluate the effects on proteolytic activity in Afifella marina strain ME under anaerobic condition. After that, the effect of photoperiods on proteolytic activity was monitored under anaerobic light condition (3000 lux) at 0 h (0L/24D), 6 h (6L/18D), 12 h (12L/12D), 18 h (18L/6D) and 24 h (24L/0D) of photoperiod. The highest proteolytic activity of 74.67 U was recorded at 3000 lux illumination light intensity. The proteolytic activity in bacterium Afifella marina strain ME was positively associated with the dry cell weight. The proteolytic activity of 72.67 U in bacterium Afifella marina strain ME at 18 h (18L/6D) photoperiod is not significantly different (p > 0.05) from proteolytic activity of 74.67 U recorded at continuous light (24L/0D) condition. Light intensity of 3000 lux, culture period of 48 h and a photoperiod of 18 h (18L/ 6D) were the optimum parameters for proteolytic activity in bacterium Afifella marina strain ME.
Cite this paper: Al-Azad, S. , Soon, T. and Ransangan, J. (2013) Effects of light intensities and photoperiods on growth and proteolytic activity in purple non-sulfur marine bacterium, Afifella marina strain ME (KC205142). Advances in Bioscience and Biotechnology, 4, 919-924. doi: 10.4236/abb.2013.410120.

[1]   Srinivas, T.N.R., Anil Kumar, P., Sasikala, C., Ramana, C.V. and Imhoff, J.F. (2007) Rhodobacterium from tidal waters, and emended description of the genus Rhodobacter. International Journal of Systematic and Evolutionary Microbiology, 57, 1984-1987.

[2]   Imhoff, J.F. and Hiraishi, A. (2005) Genus I. Rhodobium, In: Garrity, G.M., Brenner, D.J., Krieg, N.R. and Staley, J.T., Eds., Bergey’s Manual of Systematic Bacteriology, Vol. 2, Part C: The Alpha-, Beta-, Delta-and EpsilonproTeobacteria, Springer, NewYork, 571-574.

[3]   Hiraishi, A., Urata, K. and Satoh, T. (1995) A new genus of marine budding phototrophic bacteria, Rhodobium gen. nov., which includes Rhodobium orientis sp. nov. and Rhodobium marinum comb. nov. International Journal of Systematic Bacteriology, 45, 226-234.

[4]   Imhoff, J.F. and Truper, H.G. (1991) A hand book on the biology of bacteria: Ecophysiology, isolation, identification, and application. Springer Verlas, New York.

[5]   Azad, S.A., Vikineswary, S., Chong, V.C. and Ramachandran, K.B. (2003) Rhodovulum sulfidophilum in the treatment and utilization of sardine processing wastewater. Letters in Applied Microbiology, 38, 13-18.

[6]   Azad, S.A., Chong, V.C. and Vikineswary, S. (2002) Phototrophic bacteria as feed suplliment for rearing Penaeus monodon larvae. Journal of the World Aquaculture Society, 33, 991-994.

[7]   Tsygankov, A.A., Fedorov, A.S., Talipova, L.V., Laurinavichene, T.V., Miyake, J. and Gogotov, I.N. (1998) Use of immobilized phototrophic microorganisms for wastewater treatment and simultaneous production of hydrogen. Applied Biochemistry and Microbiology, 34, 362-366.

[8]   Hirotani, H., Ohigashi, H., Kobayashi, M., Koshinizu, K. and Takashi, E. (1991) Inactivation of T5 phage by cisvaccenic acid and antivirus substances from Rhodopseudomonas capsulate, and by unsaturated fatty acids and related alcohols. FEMS Microbiology Letters, 77, 13-18.

[9]   Brandl, H., Cross, R.A., Lenz, R.W., Lloyd, R. and Fuller, R.C. (1991) The accumulation of poly (3-hydroxyalkanoates) in Rhodobacter sphaeroides. Archieves of Microbiology, 155, 337-340.

[10]   Vatsala, T.M. (1987) Uptake of metal ions by photosynthetic bacterium. Current Science, 56, 1225-1226.

[11]   Noparatnaraporn, N. and Nagai, S. (1986) Selection of Rhodobacter sphaeroides P47 as a useful source of single cell protein. Journal of General and Applied Microbiology, 1, 351-359.

[12]   Tielen, P., Rosenau, F., Wilhelm, S., Jaeger, K.E., Flemming, H.C. and Wingender, J. (2010) Extracellular enzymes affect biofilm formation of mucoid Pseudomonas aeruginosa. Microbiology, 156, 2239-2252.

[13]   Oda, K., Tanskull, S., Oyama, H. and Noparatnaraporn, N. (2004) Purification and characterization of alkaline serine proteinase from photosynthetic bacterium, Rubrivivax gelatinosus KDDS1. Bioscience, Biotechnology and Biochemistry, 68, 650-655.

[14]   Prakash, B., Veeregowda, B.M. and Krishnappa, G. (2003) Biofilms: A survival strategy of bacteria. Current Science 85, 1299-1307.

[15]   Hanada, S.Y., Kawasw, A., Hiraishi, S., Takaichi, K., Matsuura, K., Shimada, and Nagashima, K.V.P. (1997) Porphyrobacter terpidarius sp. nov., a moderately thermophilic aerobic photosynthetic bacterium isolated from a hot spring. International Journal of Systematic and Evolutionary Microbiology, 47, 408-413.

[16]   Chrost, R.J. (1991) Environmental control of the synthesis and activity of aquatic microbial ectoenzymes. In: Chrost, R.J., Ed., Microbial Enzymes in Aquatic Environments. Springer, Berlin, Heidelberg, New York, 29-59.

[17]   Prasertsan, P., Choorit, W. and Suwanno, S. (1993) Optimisation for growth of Rhodocyclus gelatinosus in seafood processing effluents. World Journal of Microbiology and Biotechnology, 9, 593-596.

[18]   Willerding, A.L., de Oliveira, L.A., Moreira, F.W., Germano, M.G. and Chagas, A.F. (2011) Lipase activity among bacteria isolated from amazonian soils. Enzyme Research, 2011, Article ID: 720194.

[19]   Jain, D., Pancha, I., Mishra, S.K., Shrivastav, A. and Mishra, S. (2011) Purification and characterization of haloalkaline thermoactive, solvent stable and SDS-induced protease from Bacillus sp.: A potential additive for laundry detergents. Bioresource Technology, 115, 228-236.

[20]   Sawada, H., Parr, R.C. and Roger, P.L. (1977) Photosynthetic bacteria in waste water treatment. Journal of Fermentation Technology, 55, 326-336.

[21]   Winkler, U.K. and Stuckman, M. (1978) Glycogen, hyaluronate, and some other polysaccharides greatly enhance the formation of exolipase by serratia marcescens. Journal of Bacteriology, 138, 663-670.

[22]   Wang, Y. (2011) Use of probiotics Bacillus coagulans, Rhodopseudomonas palustris and Lactobacillus acidophilus as growth promoters in grass carp (Ctenopharyngodon idella) fingerlings. Aquaculture Nutrition, 17, 372-78.

[23]   Ainon, H., Tan, C.J. and Vikineswary, S. (2006) Biological characterization of Rhodomicrobium vannielii isolated from a hot spring at Gadek, Malacca, Malaysia. Malaysian Journal of Microbiology, 2, 15-21.

[24]   Shivanand, P. and Jayaraman, G. (2009) Production of extracellular protease from halotolerant bacterium, Bacillus aquimaris strain VITP4 isolated from Kumta coast. Process Biochemistry, 44, 1088-1094.

[25]   Boominadhan, U., Rajakumar, R., Sivakumaar, P.K.V. and Joe, M.M. (2009) Optimization of protease enzyme production using Bacillus sp. isolated from different wastes. Botany Research International, 2, 83-87.

[26]   Bairagi, A., Ghosh, K.S., Sen, S.K. and Ray, A.K. (2002) Enzyme producing bacterial flora isolated from fish digestive tracts. Aquaculture International, 10, 109-121.

[27]   Lensch, M., Herrmann, R.G., and Sokolenko, A. (2001) Identification and characterization of SppA, a novel light inducible chloroplast proteinase complex associated with thylakoid membranes. Journal of Biology and Chemistry, 276, 33645-33651.

[28]   Singh, S. and Das, S. (2011) Screening, production, optimization and characterization of cyanobacterial polysaccharide. World Journal of Microbiology and Biotechnology, 27, 1971-1980.

[29]   Eroglu, E., Gunduz, U., Yucel, M. and Eroglu, I. (2010) Photosynthetic bacterial growth and productivity under continuous illumination or diurnal cycles with olive mill wastewater as feedstock. International Journal of Hydrogen Energy, 35, 5293-5300.

[30]   Liqin, S., Changhai, W. and Lei, S. (2008) Effects of light regime on extracellular polysaccharide production by Porphyridium cruentum cultured in flat plate photobioreactors. The 2nd International Conference on Bioinformatics and Biomedical Engineering, ICBBE 2008, Shanghai, 16-18 May 2008, 1488-1491.