ABSTRACT The polyhedrin (polh) gene is often used to analyse evolution of baculovirus. In this report, the polh of Antheraea pernyi nucleopolyhedro-virus (AnpeNPV) was cloned and sequenced. The Open reading frame (ORF) of the AnpeNPV consists of 738 nucleotides encoding 245 amino acids with molecular masses of 29 kDa. The deduced amino acids were significant homol-ogy with other baculoviruses, such as Attacus ricini NPV (ArNPV) and Autographa californica NPV (AcNPV). A strongly hydrophilic region was predicted at positions from 30 to 50 of the An-peNPV Polh protein by bioinformatics analysis. Expression of the polh gene of AnpeNPV in E. coli was examined by SDS–PAGE, Western blot and Mass-spectrum analysis. The result showed that the bacterium expression system was suitable for the virus gene expression. It indi-cated that the products of the polh gene ex-pressed in this system can be easier to use for raising antibodies.
Cite this paper
nullHuang, J. , Wu, H. , Wu, Y. , Zhu, S. and Wang, W. (2009) Analysis and expression of the polyhedrin gene of Antheraea pernyi nucleopolyhedrovirus (AnpeNPV). Journal of Biomedical Science and Engineering, 2, 128-134. doi: 10.4236/jbise.2009.22023.
 Q. Fan, S. Li, L. Wang, B. Zhang, B. Ye, Z. Zhao, Cui, L. (2007). The genome sequence of the multinucleocapsid nucleo-polyhedrovirus of the Chinese oak silkworm Antheraea pernyi. Virology 366(2), 304-315.
 O.A. Lihoradova, I. D. Ogay, A. A. Abdukarimov, S. S. Azi-mova, D. E. Lynn, Slack, J. M. (2007). The Homingbac bacu-lovirus cloning system: An alternative way to introduce foreign DNA into baculovirus genomes. J Virol Methods 140 (1-2), 59-65.
 Z. M. Nie, Z. F. Zhang, D. Wang, P. A. He, C. Y. Jiang, L. Song, F. Chen, J. Xu, L. Yang, L. L. Yu, J.Chen, Z. B. Lv, J. J. Lu, X. F. Wu, Zhang Y. Z. (2007) Complete sequence and organization of Antheraea pernyi nucleopolyhedrovirus, a dr-rich baculovirus. BMC Genomics 8, 248-261.
  S. P. Cook, R. E. Webb, J. D. Podgwaite, Reardon, R. C. (2003) Increased mortality of gypsy moth Lymantria dispar (L.) (Lepi-doptera: Lymantriidae) exposed to gypsy moth nuclear polyhe-drosis virus in combination with the phenolic gycoside salicin. J Econ Entomol 96(6), 1662-1667.
 Moscardi, F. (1999) Assessment of the application of baculovi-ruses for control of Lepidoptera. Annu Rev Entomol 44, 257-289.
 X. Dai, T. M. Stewart, J. A. Pathakamuri, Q. Li, Theilmann, D. A. (2004) Autographa californica multiple nucleopolyhedrovirus
 S. G. Kamita, S. Maeda, Hammock, B. D. (2003) High-fre-quency homologous recombination between baculoviruses in-volves DNA replication. J Virol 77(24), 13053-13061.
 A. M. Khurad, A. Mahulikar, M. K. Rathod, M. M. Rai, S. Kanginakudru, Nagaraju J. (2004) Vertical transmission of nu-cleopolyhedrovirus in the silkworm, Bombyx mori L. Journal of Invertebrate Pathology 87, 8–15.
 S. Gomi, C. E. Zhou, W. Y. Yih, K. Majima, Maeda S. (1997) Deletion analysis of four of eighteen late gene expression factor gene homologues of the baculovirus, BmNPV. Virology 230, 35-47.
 W. B. Wang, S. Y. Zhu, L. Q. Wang, F. Yu, Shen W. D. (2005) Cloning and sequence analysis of the Antheraea pernyi nucleo-polyhedrovirus gp64 gene. J Biosci 30, 605-610.
 L. H. Robert, Bonning B.C. (2003) Comparative analysis of the genomes of Rachiplusia ou and Autographa californica multiple nucleopolyhedroviruses Journal of General Virology 84, 1827–1842.
 S. Gomi, K. Majima, Maeda S. (1999) Sequence analysis of the genome of Bombyx mori nucleopolyhedrovirus. J Gen. Virol. 80, 1323-1337.
 M. D. Ayres, S. C. Howard, J. Kuzio, M. Lopez-Ferber, Possee R.D. (1994) The complete DNA sequence of Auto-grapha californica nuclear polyhedrosis virus. Virology 202, 586-605.
 E. A. van Strien, D. Zuidema, R.W. Goldbach, Vlak, J. M. (1992) Nucleotide sequence and transcriptional analysis of the polyhedrin gene of Spodoptera exigua nuclear polyhedrosis vi-rus. J Gen Virol 73 ( Pt 11), 2813-2821.
 P. Y. Chou, Fasman, G. D. (1977) Beta-turns in proteins. J Mol Biol 115(2), 135-175.
 E. B. Carstens, A. Krebs, Gallerneault, C. E. (1986) Identifica-tion of an amino acid essential to the normal assembly of Auto-grapha californica nuclear polyhedrosis virus polyhedra. J Virol 58(2), 684-688.
 T. P. Hopp, Woods, K. R. (1981). Prediction of protein antigenic determinants from amino acid sequences. Proc Natl Acad Sci U S A 78(6), 3824-3828.
 Rohrmann, G. F. (1986) Polyhedrin structure. J Gen Virol 67(8), 1499-1513.
 R. D. Possee, S. C. Howard, (1987) Analysis of the polyhedrin gene promoter of the Autographa californica nuclear polyhedro-sis virus. Nucleic Acids Res 15(24), 10233-10248.
 G. E. Smith, M. J. Fraser, Summers, M. D. (1983) Molecular engineering of the Autographa californica nuclear polyhedrosis virus genome:deletion mutations within the polyhedrin gene. J Virol 46, 584-593.