ACES  Vol.3 No.4 A , October 2013
Optimal Parameters for in Vitro Development of the Fungus Hydrocarbonoclastic Penicillium sp
ABSTRACT


México has extensive areas that have been impacted by oil spills for several decades. Current bioremediation technologies mostly used microorganisms to decontaminate sites with hydrocarbons. This research evaluated the conditions for the optimal development of the strain of a hydrocarbonoclastic fungus, which was found in samples of soil contaminated with 4.0 × 105 mg·kg-1 of Total Petroleum Hydrocarbons (TPH). A completely randomized experimental design with a 3 × 3 × 4 factor arrangement was used: three levels of temperature (T1 = 29, T2 = 35 and T3 = 40), three of pH (pH1 = 3.5, pH2 = 5.0 and pH3 = 6.0) and four nutrients (N1 = Urea, N2 = Triple-17, N3 = Nitrophoska-Blue and N4 = Pure-Salts). Total fungi were isolated from the sampled soil and were sown in a combined carbon medium for hydrocarbonoclastic fungi and a strain was selected to be adapted to a liquid mineral medium. The selected strain was classified as Penicillium sp. Analyses of variance and mean tests were performed, using the SPSS-11.0 statistical software. The microorganisms showed the highest population growth in the treatment N2pH2T1, which reached a value of 2.1 × 106 CFU·mL-1 in a biorreactor. To reach it, by bioaugmentation, the same development of Penicillium sp. in a conditioned soil would allow to implement a bioremediation strategy with great potential to retrieve soil contaminated with hydrocarbons both in Tabasco and in general in Mexico.



Cite this paper
M. Ojeda-Morales, M. Hernández-Rivera, J. Martínez-Vázquez, Y. Córdova-Bautista and Y. Hernández-Cardeño, "Optimal Parameters for in Vitro Development of the Fungus Hydrocarbonoclastic Penicillium sp," Advances in Chemical Engineering and Science, Vol. 3 No. 4, 2013, pp. 19-29. doi: 10.4236/aces.2013.34A1004.
References
[1]   INEGI, “Comunicado Núm. 203/09,” INEGI, 2009.

[2]   N. Cruz-Serrano, “Pemex, tercera petrolera en el mundo en 2009,” El Universal, 2013. http://www.eluniversal.com.mx/finanzas/80420.html

[3]   E. C. Hernández, “Innovación Tecnológica Base Para Extracción de Petróleo,” Milenio Tabasco, 2013. http://www.skyscrapercity.com/showthread.php?t=634607&page=18

[4]   S. Arias, “Desarrollo Económico de Tabasco,” Tabasco hoy, 2013. http://www.tabascohoy.com.mx/noticia.php?id_nota=190648

[5]   M. Levin and M. Gealt, “Biotratamiento de Residuos Tóxicos y Peligrosos,” McGraw-Hill. Madrid, 1997.

[6]   J. Eweis, S. Ergas, D. Chag and E. Schoroeder, “Principios de Biorrecuperación,” McGraw-Hill, Madrid, 1999.

[7]   K. Watanabe, “Microorganisms Relevant to Bioremediation,” Current Opinion in Biotechnology, Vol. 12, No. 3, 2001, pp. 237-241. http://dx.doi.org/10.1016/S0958-1669(00)00205-6

[8]   W. T. Jr. Frankenberger, “The Need for a Laboratory Feasibility Study in Bioremediation of Petroleum Hydrocarbons,” In: E. J. Calabrese andP. T. Kostecki, Eds., Hydrocarbon Contaminated Soils and Groundwater, Lewis Publication, Boca Raton, 1992, pp. 237-293.

[9]   Y. Castro-Riquelme, “Estudios de Toxicidad y Biodegradacion de Hidrocarburos Modelo en Hongos Filamentosos,” Maestría en Biotecnología Dissertation. Universidad Autónoma Metropolitana, México, D.F., 2008.

[10]   M. V. Walter, “Bioaugmentation,” In: C. J. Hurst, Ed., Manual of Environmental Microbiology, ASM Press, Washington DC, 1997, pp. 753-765.

[11]   R. M. Atlas and R. Unterman, “Bioremediation,” In: A. L. Demain and J. E. Davies, Eds., Manual of Industrial Microbiology and Biotechnology, 2nd Edition, ASM Press, Washington DC, 1999, pp. 666-681.

[12]   S. Boonchan, M. L. Britz and G. A. Stanley, “Degradation and Mineralization of High-Molecular-Weight Polycyclic Aromatic Hydrocarbons by Defined Fungal-Bacterial Cocultures,” Applied and Environmental Microbiology, Vol. 66, No. 3, 2000, pp. 1007-1019. http://dx.doi.org/10.1128/AEM.66.3.1007-1019.2000

[13]   S. Barathi and N. Vasudevan, “Utilization of Petroleum Hydrocarbons by Pseudomonas fluorescens Isolated from a Petroleum-Contaminated Soil,” Environment International, Vol. 26, No. 5-6, 2001, pp. 413-416. http://dx.doi.org/10.1016/S0160-4120(01)00021-6

[14]   E. Seklemova, A. Pavlova and K. Kovacheva, “Biostimulation Based Bioremediation of Diesel Fuel: Field Demonstration,” Biodegradation, Vol. 12, No. 5, 2001, pp. 311-316. http://dx.doi.org/10.1023/A:1014356223118

[15]   I. Kuiper, E. L. Lagendijk, G. O. Bloemberg and B. J. J. Lugtenberg, “Rhizoremediation. A Beneficial Plant Microbe Interaction,” Molecular Plant-Microbe Interactions, Vol. 17, No. 1, 2004, pp. 6-15. http://dx.doi.org/10.1094/MPMI.2004.17.1.6

[16]   T. E. Ogbulie, H. C. Nwigwe, M. O. E. Iwuala and G. C. Okpokwasili, “Study on the Use of Monoculture and Multispecies on Bioaugumentation of Crude Oil Contaminated Agricultural Soil,” Nigerian Journal of Microbiology, Vol. 24, 2010, pp. 2160-2167.

[17]   A. F. Gesinde, E. B. Agbo, M. O. Agho and E. F. C. Dike, “Bioremediation of Some Nigerian and Arabian Crude Oils by Fungal Isolates,” International Journal of Pure and Applied Sciences, Vol. 2, No. 3, 2008, pp. 37-44.

[18]   A. D’Annibale, F. Rosetto, V. Leonardi, F. Federici and M. Petruccioli, “Role of Autochthonous Filamentous Fungi in Bioremediation of a Soil Historically Contaminated with Aromatic Hydrocarbons,” Applied and Environmental Microbiology, Vol. 72, No. 1, 2006, pp. 28-36. http://dx.doi.org/10.1128/AEM.72.1.28-36.2006

[19]   Conestoga-Rovers & Associates (CRA), “Bioaugmentation,” Innovative Technology Group, Vol. 3, No. 4, 2003, pp. 1-2.

[20]   J. B. Davis, “Petroleum Microbiology,” Elsevier, Amsterdam, 1967.

[21]   J. A. Bumpus, “Biodegradation of Polycyclic Aromatic Hydrocarbons by Phanerochaete chrysosporium,” Applied and Environmental Microbiology, Vol. 55, No. 1, 1989, pp. 154-158.

[22]   T. S. Brodkorb and R. L. Legge, “Enhanced Biodegradation of Phenanthrene in Oil Tar-Contaminated Soils Supplemented with Phanerochaete chrysosporium,” Applied and Environmental Microbiology, Vol. 58, No. 9, 1992, pp. 3117-3121.

[23]   J. L. Solórzano-Lemos, A. C. Rizzo, V. S. Millioli, A. U. Soriano, M. I. De Moura-Sarquis and R. Santos, “Petroleum Degradation by Filamentous Fungi,” Contribuicao Técnica a 9th International Petroleum Environmental Conference, Novo México, 2002, pp. 21-25.

[24]   H. Sing, “Mycoremediation,” John Wiley & Sons, Inc., Hoboken, 2006. http://dx.doi.org/10.1002/0470050594

[25]   A. L. Leitao, “Potential of Penicillium Species in the Bioremediation Field,” International Journal of Environmental Research and Public Health, Vol. 6, No. 4, 2009, pp. 1393-1417. http://dx.doi.org/10.3390/ijerph6041393

[26]   H. M. Hussein and Y. R. Abdel-Fattah, “Numerical Modelling of Petroleum Oil Bioremediation by a Local Penicillium Isolate as Affected with Culture Conditions: Appli cation of Plackett-Burman Design,” Arab Journal of Bio technology, Vol. 5, No. 2, 2002, pp. 165-172.

[27]   A. Mittal and P. Singh, “Studies on Biodegradation of Crude Oil by Aspergillus niger,” The South Pacific Jounal of Natural and Applied Sciences, Vol. 27, No. 1, 2009, pp. 57-60.

[28]   O. Obire and E. C. Anyanwu, “Impact of Various Concentrations of Crude Oil on Fungal Populations of Soil,” International Journal of Environmental Science and Technology, Vol. 6, No. 2, 2009, pp. 211-218.

[29]   C. C. Isitua and I. N. Ibeh, “Comparative Study of Aspergillus niger and Penicillium sp. in the Biodegradation of Automotive Gas Oil (AGO) and Premium Motor Spirit (PMS),” African Journal of Biotechnology, Vol. 9, 2010, pp. 3607-3610.

[30]   E. Kiehlmann, L. Pinto and M. Moore, “The Transformation of Chrysene to Trans-1,2-dihydroxy-1,2-dihydrochrysene by Filamentous Fungi,” Canadian Journal of Microbiology, Vol. 42, 1996, pp. 604-608. http://dx.doi.org/10.1139/m96-081

[31]   C. Machín-Ramírez, D. Morales, F. Martínez-Morales, A. I. Okoh and M. R. Trejo-Hernández, “Benzo[a]pyrene removal by Axenic- and Co-Cultures of Some Bacterial and Fungal Strains,” International Biodeterioration and Biodegradation, Vol. 64, No. 7, 2010, pp. 538-544. http://dx.doi.org/10.1016/j.ibiod.2010.05.006

[32]   H. J. Van den Brink, R. F. M. van Gorcom, C. A. M. J. J. van den Hondel and P. J. Punt, “Cytochrome P450 Enzyme Systems in Fungi,” Fungal Genetics and Biology, Vol. 23, No. 1, 1998, pp. 1-17. http://dx.doi.org/10.1006/fgbi.1997.1021

[33]   C. E. Cerniglia and J. B. Sutherland, “Bioremediation of Polycyclic Aromatic Hydrocarbons by Ligninolytic Fungi,” In: G. M. Gadd, Ed., Fungi in Bioremediation, Cambridge University Press, Cambridge, 2001, pp. 136-187. http://dx.doi.org/10.1017/CBO9780511541780.008

[34]   J. A. Field, E. Jong, G. F. Cost and J. A. M. Bont, “Biodegradation of Polycyclic Aromatic Hydrocarbons by New Isolates of White Rot Fungi,” Applied and Environmental Microbiology, Vol. 58, No. 7, 1992, pp. 2219-2226.

[35]   L. Launen, L. Pinto, C. Wiebe, E. Kiehlmann and M. Moore, “The Oxidation of Pyrene and Benzo[a]pyrene by nonbasidiomycete Soil Fungi,” Canadian Journal of Microbiology, Vol. 41, No. 6, 1995, pp. 477-488. http://dx.doi.org/10.1139/m95-064

[36]   L. Launen, L. Pinto and M. Moore, “Optimization of Pyrene Oxidation by Penicillium janthinellum Using Response-Surface Methodology,” Applied Microbiology and Biotechnology, Vol. 51, No. 4, 1999, pp. 510-515. http://dx.doi.org/10.1007/s002530051425

[37]   T. Wunder, J. Marr, S. Kremer, O. Sterner and H. Anke, “1-Methoxypyrene and 1,6-Dimethoxypyrene: Two Novel Metabolites in Fungal Metabolism of Polycyclic Aromatic Hydrocarbons,” Archives of Microbiology, Vol. 167, 1997, No. 5, pp. 310-316. http://dx.doi.org/10.1007/s002030050449

[38]   B. Chávez-Gómez, R. Quintero, F. Esparza-García, A. M. Mesta-Howard, D. F. J. Zavala, C. H. Hernández-Rodríguez, T. Gillén, H. M. Poggi-Varaldo, J. Barrera-ortés and R. Rodríguez-Vázquez, “Removal of Phenanthrene from Soil by Co-Cultures of Bacteria and Fungi Pregrown on Sugarcane Bagasse Pith,” Bioresource Technology, Vol. 89, No. 2, 2003, pp. 177-183. http://dx.doi.org/10.1016/S0960-8524(03)00037-3

[39]   F. E. Valenzuela, M. L. Solís, V. O. Martínez and T. D. Pinochet, ”Hongos Aislados Desde Suelos Contaminados Con Petróleo,” Boletín Micológico, Vol. 21, 2006, pp. 35-41.

[40]   M. C. Rivera-Cruz, “Microorganismos Rizosféricos de Los Pastos Alemán (Echinochloa polystachya H.B.K. Hitchc) y Cabezón (Paspahum virgatum L.) en la Degradación Del Petróleo Crudo y el Benzo(a)pireno,” Ph.D. Dissertation, Colegio de Postgraduados, Montecillo, 2001.

[41]   INEGI, “Síntesis Geográfica, Nomenclátor y Anexo Cartográfico del Estado de Tabasco,” Instituto Nacional de Estadística y Geografía, Aguascalientes, 2001.

[42]   SEMARNAT, “Norma Oficial Mexicana NOM-021-RECNAT-2000. Apartado 6.1. Evaluación de la Conformidad Para Muestreo de Suelos. Muestreo Para Determinar Fertilidad de Suelos,” Diario Oficial de la Federación, 2nd. Secc., México D.F., 2002.

[43]   USEPA, “EPA-Method-3540C. Soxhlet Extraction. Hidrocarburos Totales Del Petróleo (Fracción Pesada),” 1996. http://www.epa.gov/wastes/hazard/testmethods/sw846/pdfs/3540c.pdf

[44]   USEPA, “EPA-Method-9071B. n-Hexane Extractable Material (hem) for Sludge, Sediment, and Solid Samples,” 1998. http://www.caslab.com/EPA-Methods/PDF/EPA-Method-9071B.pdf

[45]   USEPA, “EPA-Method-1664A. Revition A. n-Hexane Extractable Material,” 1999. http://www.epa.gov/waterscience/methods/method/oil/1664guide.pdf

[46]   M. T. Madigan, J. M. Martinko, P. V. Dunlap and D. P. Clark, “Biología de Los Microorganismos,” Pearson, Addison Wesley, Madrid, 2009.

[47]   M. C. Rivera-Cruz, R. Ferrera-Cerrato, R. Rodríguez-Vásquez and L. Fernández-Linares, “Adaptación y Selección de Microorganismos Autóctonos en Médios de Cultivos Enriquecidos Com Petróleo Crudo,” Terra Latinoamericana, Vol. 20, No. 4, 2002, pp. 423-444.

[48]   H. Corvantes, “PEMEX: El Petróleo,” Petróleos Mexicanos, 1988.

[49]   H. Barnett and B. Hunter, “Illustrated Genera of Imperfect Fungi,” Burgess Pub. Company, Minnesota, 1972.

[50]   M. Kastner, M. Breuer-Jammali and B. Mhro, “Enumeration and Characterization of the Soil Sites to Mineralize Polycyclie Aromatic Hidrocarbons,” Applied Microbiology and Biotechnology, Vol. 41, No. 2, 1994, pp. 267-273. http://dx.doi.org/10.1007/BF00186971

[51]   G. Y. Pica, A. Ronco and B. M. Díaz, “Bioensayo de Toxicidad Crónica con Selenastrum capricornutum (Pseudokirchneriella subcapitata). Método de Enumeración Celular Basado en el Uso de Hematocímetro Neu bauer,” In: M. G. Castillo, Ed., Ensayos Toxicológicos y Métodos de Evaluación de Calidad de Aguas, Estandarización, intercalibración, Resultados y Aplicaciones, Instituto Mexicano de Tecnología del Agua, 2004, pp. 62-73.

[52]   J. Zavala-Cruz, V. A. Botello, S. R. H. Adams and A. Ruiz-Bello, “Hidrocarburos Alifáticos y Aromáticos en las Tierras,” In: J. Zavala-Cruz, M. C. Gutiérrez-Castorena and D. J. Palma-López, Eds., Impacto Ambiental en Las Tierras Del Campo Petrolero Samaria, Colegio de Postgraduados, CONACYT, CCYTET, Villahermosa, Tabasco, 2003, pp. 131-140.

[53]   M. R. Atlas, A. Horowitz, M. Krichevky and K. A. Bej, “Response of Microbial Population to Environmental Disturbance,” Microbial Ecology, Vol. 22, No. 1, 1991, pp. 249-256. http://dx.doi.org/10.1007/BF02540227

[54]   R. H. Adams-Schroeder, V. I. Domínguez-Rodríguez and L. Vinalay-Carrillo, “Evaluation of Microbial Respiration and Ecotoxicity in Contaminated Soils Representative of the Petroleum Producing Region of Southeastern México,” Terra, Vol. 20, No. 3, 2002, pp. 253-265.

[55]   C. E. Cerniglia and M. A. Heitkamp, “Microbial Degradation of Polycyclic Aromatic Hydrocarbon (PAH) in the Aquatic Environment,” In: U. Varausi, Ed., Metabolism of PAH in the Aquatic Environment, CRC Press Inc., Boca Raton, 1987, pp. 41-68.

[56]   J. E. Heidelberg, I. I. Paulsen, K. E. Nelson, E. J. Gaidos, W. C. Nelson, T. D. Read and J. A. Eison, “Genome Sequence of the Dissimilatory Metal Ion-Reducing Bacterium. Shewanella oneidensis,” Nature and Biotechnology, Vol. 1, 2002, pp. 1-6.

[57]   J. Gilman, “Manual de Los Hongos Del Suelo,” Companía Editorial Continental S.A, México D.F., 1963.

[58]   G. Smith, “Introducción a la Micología Industrial,” Editorial Acribia, Espana, 1963.

[59]   T. Mier, C. Toriello and M. Ulloa, “Hongos Microscópicos Saprobios y Parásitos: Métodos de Laboratorio,” UAM-UNAM, México D. F., 2000.

[60]   S. S. Radwan, N. A. Sorkhoh, F. Fardoun and R. H. Al-Hasan, “Soil Management Enhancing Hydrocarbon Degradation in the Polluted Kuwaitii Desert,” Applied Microbiology and Biotechnology, Vol. 44, No. 1-2, 1995, pp. 265-270. http://dx.doi.org/10.1007/BF00164513

[61]   T. M. April, J. M. Foght and R. S. Currah, “Hydrocarbon-Dagrading Filamentous Fungi. Isolated from Flare Pit Soils in Northern and Western Canada,” Canadian Journal of Microbiology, Vol. 46, No. 1, 2000, pp. 38-49.

[62]   G. Nkwelang, F. L. Kamga, E. Nkeng and S. P. Antai, “Studies on the Diversity, Abundance and Succession of Hydrocarbon Utilizing Micro Organisms in Tropical Soil Polluted with Oily Sludge,” African Journal of Biotechnology, Vol. 8, 2008, pp. 1075-1080.

[63]   K. Jeonge-Dong and L. Choul-Gyun, “Microbial Degradation of Polycyclic Aromatic Hydrocarbons in Soil by Bacterium-Fungus Co-Cultures,” Biotechnology and Bioprocess Engineering, Vol. 12, No. 4, 2007, pp. 410-416. http://dx.doi.org/10.1007/BF02931064

[64]   O. Obire, E. C. Anyanwu and R. N. Okigbo, “Saprophytic and Crude Oil Degrading Fungi from Cow Dung and Poultry Droppings as Bioremediating Agents,” Journal of Agricultural Science and Technology, Vol. 4, 2008, pp. 81-89.

[65]   M. A. Hernández-Rivera, M. E. Ojeda-Morales, J. G. Martínez-Vázquez, V. Villegas-Cornelio and Y. CórdovaBautista, “Optimal Parameters for in Vitro Development of the Hydrocarbonoclastic Microorganism Proteus sp.,” Journal of Soil Science and Plant Nutrition, Vol. 11, No. 1, 2011, pp. 29-43.

[66]   C. C. Okoro, “Biodegradation of Hydrocarbons in Untreated Produce Water Using Pure Fungal Cultures,” African Journal of Microbiology Research, Vol. 2, 2008, pp. 217-223.

[67]   K. Lee and E. M. Levy, “Biodegradation of Petroleum in the Marine Environment and Its Enhancement,” In: J. O. Nriagu and J. S. S. Lakshminarayana, Eds., Aquatic Toxicology and Water Quality Management, John Wiley and Sons Inc., New York, 1989, pp. 217-243.

[68]   B. T. Walton, E. A. Guthrie and A. M. Hoylman, “Toxicant Degradation in the Rhizosphere,” In: T. A. Anderson, and J. R. Coats, Eds., Bioremediation through Rhizosphere Technology, American Chemical Society, Washington DC, 1994, pp. 11-26. http://dx.doi.org/10.1021/bk-1994-0563.ch002

[69]   E. Rosenberg and E. Z. Ron, “Bioremediation of Petroleum Contamination,” In: R. L. Crawford and D. L. Crawford, Eds., Bioremediation: Principles and Applications, Cambridge University Press, Cambridge, 1998, pp. 100-124.

[70]   J. C. Méndez-Zavala, F. Contreras, R. Lara, R. Victoriano and R. Rodríguez, “Producción Fúngica de un Pigmento Rojo Empleando la Cepa Xerofilica Penicillium purpurogenum GH-2,” Revista Mexicana de Ingeniería Química, Vol. 6, 2007, pp. 267-273.

[71]   Y. J. Cho, J. P. Park, H. J. Hwan, S. W. Kim, J. W. Choi and J. W. Yun, “Production of Red Pigment Bye Submerged Culture of Paecilomyces sinclairii,” Letters in Applied Microbiology, Vol. 35, No. 3, 2002, pp. 195-202. http://dx.doi.org/10.1046/j.1472-765X.2002.01168.x

[72]   Y. J. Cho, H. J. Hwang, S. N. Kim, C. H. Song and J. W. Yun, “Effect of Carbon Source and Aeration Rate on Broth Rheology and Fungal Morphology during Red Pigment Production by Sinclairii in Batch Bioreactor,” Journal of Biotechnology, Vol. 95, No. 1, 2002, pp. 13-23.

[73]   M. Alexander, “Biodegradation and Bioremediation,” Academic Press, San Diego, 1994.

[74]   A. Saraswathy and R. Hallberg, “Mycelial Pellet Formation by Penicillium ochrochloron Species Due to Exposure to Pyrene,” Microbiological Research, Vol. 160, No. 4, 2005, pp. 375-383. http://dx.doi.org/10.1016/j.micres.2005.03.001

[75]   J. E. L. Corry, “Relationships of Water Activity to Fungal Growth,” In: V. N. Reinhold, Ed., Food and Beverage Micology, Beuchat LR, New York, 1987, pp. 51-99.

[76]   J. Lacey, “Pre- and Post-Harvest Ecology of Fungi Causing Spolage of Foods and Other Stored Products,” Journal of Applied Bacteriology, Symposium Supplement, 1989, pp. 11S-25S.

[77]   E. Ercoli, J. Gálvez, M. Di Paola, J. Cantero, S. Videla, M. Medaura and J. Bauzá, “Análisis y Evaluación de Pará metros Críticos en Biodegradación de Hidrocarburos en Suelo,” In: C. Producción III, Ed., Workshop Latinoa mericano Sobre Aplicaciones de la Ciencia en la Ingeniería de Petróleo, Formato electrónico, Puerto Iguazú, 2000.

[78]   V. Sanchis, F. Lafuente, I. Vinas, M. Torres and R. Canela, “Influence of Incubation Conditions in the Patulin Production by Penicillium griseofulvum Dierckx,” Rev.

 
 
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