A rapid chromatographic
method for the determination of six phenolic acids (chlorogenic, ferulic,
gallic, p-coumaric, protocatechuic
and vanillic acids) by ultra performance liquid chromatography
(UPLC), was developed and applied for Brazilian tropical fruits mangaba
(Hancornia speciosa Gomes) and umbu (Spondias tuberosa Arruda Camara).
A multivariate statistical experimental design was employed to optimize
analytical conditions (solvent A, solvent B concentrations and flow rates).
Samples were cleaned-up by solid-phase extraction (SPE) with different solvents
(methanol and acetone) employing SPE cartridges (amine and octadecyl-silane).
The method using dihydrogen potassium phosphate 5 mmoL as solvent A and 8% acetonitrile
as solvent B presented limits of detection varying from 14 to 94 ng.mL, limits of quantification from 39 to 277 ng.mL-1 with 2 μL of injection volume while total run
time for all six compounds was only 9.6 minutes. Higher recovery was obtained
by extraction with methanol-acetone of 69.51% to 72.59% for protocatechuic acid and
69.58% to 126.31% for
the chlorogenic acid. The concentrations of chlorogenic, p-coumaric
and ferulic acids in mangaba extracts were 113.4, 32.1 and 1.5 μg.g-1, respectively while
concentrations of chlorogenic, protocatechuic, gallic, vanillic, ferulic and p-coumaric acids present in umbu fruit were 118.9, 141.3, 3.5, 2.5,
2.2 and 1.8 μg.g-1,
Cite this paper
E. Gomes, S. Ramalho, N. Gualberto, R. Miranda, N. Nigam and N. Narain, "A Rapid Method for Determination of Some Phenolic Acids in Brazilian Tropical Fruits of Mangaba (Hancornia speciosa Gomes
) and Umbu (Spondias tuberosa Arruda Camara
) by UPLC," Journal of Analytical Sciences, Methods and Instrumentation
, Vol. 3 No. 3, 2013, pp. 1-10. doi: 10.4236/jasmi.2013.33A001
 WHO—World Health Organization, “Diet, Nutrition and the Prevention of Chronic Diseases: Report of a Joint WHO/FAO Expert Consultation,” WHO Reports, Rome, 2003. http://www.who.int/en/
 M. G. L. Hertog, P. C. H. Hollman and B. Van de Putte, “Content of Potentially Anticarcinogenic Flavonoids of Tea Infusions, Wines and Fruit Juices,” Journal of the Agriculture and Food Chemistry, Vol. 41, No. 8, 1993, pp. 1242-1246. http://dx.doi.org/10.1021/jf00032a015
 B. Lapornik, M. Prosek and A. G. Wondra, “Comparison of Extracts Prepared from Plant By-Products Using Different Solvents and Extraction Time,” Journal of Food Engineering, Vol. 71, No. 2, 2005, pp. 214-222.
 T. Y. Chu, C. H. Chang, Y. C. Liao and Y. C. Chen, “Microwave-accelerated Derivatization Processes for the Determination of Phenolic Acids by Gas Chromatography-Mass Spectrometry,” Talanta, Vol. 54, No. 6, 2001, pp. 1163-1171.
 M. Nardini and A. Ghisell, “Determination of Free and Bound Phenolic Acids in Beer,” Food Chemistry, Vol. 84, No. 1, 2004, pp. 137-143.
 R. J. Robbins and S. R. Bean, “Development of a Quantitative High-Performance Liquid Chromatography-Photodiode Array Detection Measurement System for Phenolic Acids,” Journal of Chromatography A, Vol. 1038, No. 1-2, 2004, pp. 97-105.
 R. Tsao and R. Yang, “Optimization of a New Mobile Phase to Know the Complex and Real Polyphenolic Composition: Towards a Total Phenolic Index Using High-Performance Liquid Chromatography,” Journal of Chromatography A, Vol. 1018, No. 1, 2003, pp. 29-40.
 R. J. Robbins, “Phenolic Acids in Foods: An Overview of Analytical Methodology,” Journal of the Agriculture and Food Chemistry, Vol. 51, No. 10, 2003, pp. 2866-2887.
 H. S. Lee, “HPLC Analysis of Phenolic Compounds,” In: L. M. L. Nollet, Ed., Food Analysis by HPLC, 2nd Edition, Marcel Dekker, Inc., New York, 2000.
 H. M. Merken and G. R. Beecher, “Measurement of Food Flavonoids by High Performance Liquid Chromatography: A Review,” Journal of the Agriculture and Food Chemistry, Vol. 48, No. 3, 2000, pp. 577-599.
 J. G. Bordonaba, P. Crespo and L. A. Terry, “A New Acetonitrile-Free Mobile Phase for HPLC-DAD Determination of Individual Anthocyanins in Blackcurrant and Strawberry Fruits: A Comparison and Validation Study,” Food Chemistry, Vol. 129, No. 3, 2011, pp. 1265-1273.
 Y. Amakura, M. Okada, S. Tsuji and Y. Tonogai, “Determination of Phenolic Acids in Fruit Juices by Isocratic Column Liquid Chromatography,” Journal of Chromatography A, Vol. 891, No. 1, 2000, pp. 183-188.
 S. Shahrzad and I. Bitsch, “Determination of Some Pharmacologically Active Phenolic Acids in Juices by High-Performance Liquid Chromatography,” Journal of Chromatography A, Vol. 741, No. 2, 1996, pp. 223-231.
 J. Gruz, O. Novak and M. Strnad, “Rapid Analysis of Phenolic Acids in Beverages by UPLC-MS/MS,” Food Chemistry, Vol. 111, No. 3, 2008, pp. 789-794.
 A. J. P. Martin and R. L. M. Synge, “A New Form of Chromatogram Employing Two Liquid Phases,” Biochemical Journal, Vol. 35, No. 12, 1941, pp. 1358-1368.
 S. M. Munoz, M. Montserrat, B. Olga and J. Guasch, “Determination of Some Flavan-3-ols and Anthocyanins in Red Grape Seed and Skin Extracts by HPLC-DAD: Validation Study and Response Comparison of Different Standards,” Analytical Chimica Acta, Vol. 628, No. 1, 2008, pp. 104-110.
 L. Bravo, “Polyphenols: Chemistry, Dietary Sources, Metabolism and Nutritional Significance,” Nutrition Review, Vol. 56, No. 11, 1998, pp. 317-333.
 M. Markom, M. Hasan, R. W. D. Wan, H. Singh and J. M. Jahim, “Extraction of Hydrostable Tannins from Phyllanthus niruri Linn.: Effect of Solvents and Extraction Methods,” Separation and Purification Technology, Vol. 52, No. 3, 2007, pp. 487-496.
 K. A. Ross, T. Beta and S. D. Arntfield, “A Comparative Study on the Phenolic Acids Identified and Quantified in Dry Beans Using HPLC as Affected by Different Extraction and Hydrolysis Methods,” Food Chemistry, Vol. 113, No. 1, 2009, pp. 336-344.