JST  Vol.3 No.3 , September 2013
Detecting Concentration of Analytes with DETECHIP: A Molecular Sensing Array
ABSTRACT
DETECHIP is a detection system made of various sensors that has been shown to detect and discriminate between small molecules of interest, including various illicit and over-the-counter drugs. Previously, detection was normalized to a single concentration of analyte. Now this detection assay can detect concentration differences in analytes via red, green, and blue color value changes and shifts in the UV-Vis spectra of the assay. To determine the concentrations differences, the exposed assays were scanned on a flatbed scanner and the images were analyzed for individual RGB values with a custom macro in ImageJ, an image analysis program. Increasing concentrations of the analyte resulted in greater differences in color values between control and analyte wells. These differences showed a linear relationship to concentration change, some with correlation coefficients greater than 98%. This work expands the capability of DETECHIP to give information about the concentration of analyte when the analyte identity is known.

Cite this paper
H. Johnke, G. Batres, M. Wilson, A. Holmes and S. Sikich, "Detecting Concentration of Analytes with DETECHIP: A Molecular Sensing Array," Journal of Sensor Technology, Vol. 3 No. 3, 2013, pp. 94-99. doi: 10.4236/jst.2013.33015.
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