AS  Vol.4 No.9 , September 2013
Isolation and expression characterization of CBF2 in vitis amurensis with stress

The transcription factor VaCBF2, which interacts with C-repeat/DRE and its promoter, was isolated from Vitis amurensis. The VaCBF2 amino acid sequence contained a conserved AP2 domain of 56 amino acids and a potential nuclear localization sequence. The sequence of VaCBF2 showed a high level of homology with other CBF2 family members. Phylogenetic analysis showed that the amino acid sequences may be CBF2 proteins with evolutionary relationship. Quantitative reverse-transcription polymerase chain reaction analysis indicated that the expression of VaCBF2 gene in tissues (roots, stems, leaves, and petioles) was induced by low temperature, high salinity, and application of abscisic acid and salicylic acid in a time-dependent manner but to different extents in the cold-hardy V. amurensis and the less cold-hardy Vitis vinifera. The presence of cis-elements such as MYC and ABRE in VaCBF2 promoter further confirmed that this promoter was a component of the CBF transduction pathway involved in plant response to multiple stresses.

Cite this paper
Dong, C. , Tao, J. , Zhang, M. , Qin, Y. , Yu, Z. , Wang, B. , Cai, B. and Zhang, Z. (2013) Isolation and expression characterization of CBF2 in vitis amurensis with stress. Agricultural Sciences, 4, 466-472. doi: 10.4236/as.2013.49062.
[1]   Benedict, C., Skinner, J.S., Meng, R., Chang, Y., Bhalerao, R., Huner, N.P.A. and Hurry, V. (2006). The CBF1-dependent low temperature signalling pathway, regulon and increase in freeze tolerance are conserved in Populus spp. Plant, Cell and Environment, 29, 1259-1272. doi:10.1111/j.1365-3040.2006.01505.x

[2]   Chinnusamy, V., Ohta, M., Kanrar, S., Lee, B.H., Hong, X., Agarwal, M. and Zhu, J.K. (2003) ICE1: A regulator of cold-induced transcriptome and freezing tolerance in Arabidopsis. Genes Development, 17, 1043-1054. doi:10.1101/gad.1077503

[3]   Dong, C., Zhang, M., Yu, Z., Ren, J., Qin, Y., Wang, B. and Tao, J. (2013) Isolation and expression analysis of CBF4 from Vitis amurensis associated with stress. Agricultural Sciences, 4, 224-229. doi:10.4236/as.2013.45032

[4]   Dong, C., Zhang, Z., Qin, Y., Ren, J., Huang, J., Wang, B. and Tao, J. (2013) VaCBF1 from Vitis amurensis associated with cold acclimation and cold tolerance. Acta Physiologiae Plantarum, in press. doi:10.1007/s11738-013-1329-3

[5]   Haake, V., Cook, D., Riechmann, J.L., Pineda, O., Thomashow, M.F. and Zhang, J.Z. (2002). Transcription factor CBF4 is a regulator of drought adaptation in Arabidopsis. Plant Physiology, 130, 639-648. doi:10.1104/pp.006478

[6]   Jaglo, K.R., Kleff, S., Amundsen, K.L., Zhang, X., Haake, V., Zhang, J.Z. and Thomashow, M.F. (2001) Components of the Arabidopsis C-repeat/dehydration-responsive element binding factor cold-response pathway are conserved in Brassica napus and other plant species. Plant Physiol, 127, 910-917. doi:10.1104/pp.010548

[7]   Lagonigro, M.S., De Cecco, L., Carninci, P., Di Stasi, D., Ranzani, T., Rodolfo, M. and Gariboldi, M. (2004) CTABurea method purifies RNA from melanin for cDNA microarray analysis. Cell Research and the International Pigment Cell Society, 17, 312-315. doi:10.1111/j.1600-0749.2004.00155.x

[8]   Liu, J.G., Zhang, Z., Qin, Q.L., Peng, R.H., Xiong, A.S., Chen, J.M. and Yao, Q.H. (2007) Isolated and characterization of a cDNA encoding ethylene-responsive element binding protein (EREBP)/AP2-type protein, RCBF2, in Oryza sativa L. Biotechnology Letters, 29, 165-173. doi:10.1007/s10529-006-9214-4

[9]   Novillo, F., Alonso, J. M., Ecker, J. R., & Salinas, J. (2004). CBF2/DREB1C is a negative regulator of CBF1/ DREB1B and CBF3/DREB1A expression and plays a central role in stress tolerance in Arabidopsis. Proceedings of the National Academy of Sciences of USA, 101, 3985-3990. doi:10.1073/pnas.0303029101

[10]   Novillo, F., Medina, J. and Salinas, J. (2007) Arabidopsis CBF1 and CBF3 have a different function than CBF2 in cold acclimation and define different gene classes in the CBF regulon. Proceedings of the National Academy of Sciences of USA, 104, 21002-21007. doi:10.1073/pnas.0705639105

[11]   Polashock, J.J. (2010) Functional identification of a Crepeat binding factor transcriptional activator from blueberry associated with cold acclimation and freezing tolerance. Journal of the American Society for Horticultural Science, 135, 40-48.

[12]   Puhakainen, T., Li, C., Boije-Malm, M., Kangasjarvi, J., Heino, P. and Palva, E.T. (2004) Short-day potentiation of low temperature-induced gene expression of a C-repeat-binding factor-controlled gene during cold acclimation in silver birch. Plant Physiology, 136, 4299-4307. doi:10.1104/pp.104.047258

[13]   Ruelland, E., Vaultier, M.-N. Zachowski, A. and Hurry, V. (2009) Cold signalling and cold acclimation in plants. Advances in Botanical Research, 49, 35-150. doi:10.1016/S0065-2296(08)00602-2

[14]   Shinozaki, K. and Yamaguchi-Shinozaki, K. (2000) Molecular responses to dehydration and low temperature: Differences and cross-talk between two stress signaling pathways. Current Opinion in Plant Biology, 3, 217-223.

[15]   Stockinger, E.J., Gilmour, S.J. and Thomashow, M.F. (1997) Arabidopsisthaliana CBF1 encodes an AP2 domain-containing transcriptional activator that binds to the C-repeat/DRE, a cis-acting DNA regulatory element that stimulates transcription in response to low temperature and water deficit. Proceedings of the National Academy of Sciences of the USA, 94, 1035-1040. doi:10.1073/pnas.94.3.1035

[16]   Thomashow, M.F. (1999) Plant cold acclimation: Freezing tolerance genes and regulatory mechanisms. Annual Review of Plant Physiology and Plant Molecular Biology, 50, 571-599. doi:10.1146/annurev.arplant.50.1.571

[17]   Wisniewski, M., Norelli, J., Bassett, C., Artlip, T. and Macarisin, D. (2011) Ectopic expression of a novel peach (Prunuspersica) CBF transcription factor in apple (Malus x domestica) results in short-day induced dormancy and increased cold hardiness. Planta, 233, 971-983. doi:10.1007/s00425-011-1358-3

[18]   Xiao, H., Siddiqua, M., Braybrook, S. and Nassuth, A. (2006) Three grape CBF/DREB1 genes respond to low temperature, drought and abscisic acid. Plant Cell Environment, 29, 1410-1421. doi:10.1111/j.1365-3040.2006.01524.x

[19]   Yamaguchi-Shinozaki, K. and Shinozaki, K. (1994) A novel cis-acting element in an Arabidopsis gene is involved in responsiveness to drought, low-temperature, or high-salt stress. The Plant Cell Online, 6, 251-264. doi:10.1105/tpc.6.2.251

[20]   Yamaguchi-Shinozaki, K. and Shinozaki, K. (2005) Organization of cis-acting regulatory elements in osmotic-and cold-stress-responsive promoters. Trends Plant Science, 10, 88-94. doi:10.1016/j.tplants.2004.12.012

[21]   Yang, W., Liu, X.D., Chi, X.J., Wu, C.A., Li, Y.Z., Song, L.L. and Li, H.Y. (2011) Dwarf apple MbDREB1 enhances plant tolerance to low temperature, drought, and salt stress via both ABA-dependent and ABA-independent pathways. Planta, 233, 219-229. doi:10.1007/s00425-010-1279-6