FNS  Vol.4 No.9 A , September 2013
Association between Metabolic Syndrome and Erythrocyte Fatty Acid Profile in Mexican Adolescents: A Trans Fatty Acid Approach
Abstract: The type of fat consumed in the Mexican diet could predispose to the development of Metabolic Syndrome (MS) which has been associated with an increased risk to develop cardiovascular disease and type 2 diabetes mellitus. Our study included adolescents between 12 and 16 years of age, divided in two groups: Control Group (n = 31) and MS Group (n = 44). Waist circumference, blood pressure, fasting glucose, triglycerides, and HDL-cholesterol were determined. Erythrocytes’ fatty acids methyl esthers were quantified using gas chromatography with ionized flame detector. We identified 16 fatty acids (FA) with chain lengths from C12 to C24, with emphasis in four trans FA (TFA) isomers: vaccenic (C18:1n7t), elaidic (C18:1n9t), linoelaidic (C18:2n6t), and conjugated linoelaidic acids (C18:2n7t). MS Group had a less proportion of: myristic (C14), palmitoleic (C16:1), C18:1n7t, and linoleic acids (C18:2); and a higher one of C18:1n9t, C18:2n7t, and nervonic acids (C24:1) when compared to the control group. C24:1 and C18:1n9t had a significant positive association with MS (OR = 14.17 and OR = 12.94, respectively); whereas C14 (OR = 0.14), C18:1n7t (OR = 0.14), and C18:2 (OR = 0.22) appear to have a protective effect against the disease. The proportion of specific FAs in erythrocytes’ membranes differs between adolescents with MS and healthy controls; these FA not only showed a strong association with MS, but also correlated with most of its individual components. Interestingly, TFA displayed an antagonic behavior; while C18:1n9t had a strong association with MS, apparently C18:1n7t confers a protective effect; these results suggest that analyzing each TFA separately will constitute a more accurate approach to determine the role of TFAs in the pathogenesis of MS or other related metabolic disorders.
Cite this paper: M. Gabriel, M. Azucena, S. Alejandra and M. Jorge, "Association between Metabolic Syndrome and Erythrocyte Fatty Acid Profile in Mexican Adolescents: A Trans Fatty Acid Approach," Food and Nutrition Sciences, Vol. 4 No. 9, 2013, pp. 51-58. doi: 10.4236/fns.2013.49A1009.

[1]   E. D’Adamo, N. Santoro and S. Caprio, “Metabolic Syn drome in Pediatrics: Old Concepts Revised, New Con cepts Discussed,” Current Problems Pediatrics Adoles cents Health Care, Vol. 43, No. 5, 2013, pp.114-123. doi:10.1016/j.cppeds.2013.02.004

[2]   P. Zimmet, K. G. Alberti, F. Kaufman, N. Tajima, M. Silink, S. Arslanian, et al., “The Metabolic Syndrome in Children and Adolescents—An IDF Consensus Report,” Pediatric Diabetes, Vol. 8, No. 5, 2007, pp. 299-306.

[3]   D. S. Ludwig, “Childhood Obesity—The Shape of Things to Come,” The New England Journal of Medicine, Vol. 357, No. 23, 2009, pp. 2329-2371.

[4]   R. Weiss, J. Dziura, T. S. Burgert, W. V. Tamborlane, S. E. Taksali, C. W. Yeckel, et al., “Obesity and the Meta bolic Syndrome in Children and Adolescents,” The New England Journal of Medicine, Vol. 350, No. 23, pp. 2362-2374. doi:10.1056/NEJMoa031049

[5]   G. N. A. Thankamony, R. Williams and D. B. Dunger, “Metabolic Syndrome in Children Unravelled,” Paediat rics and Child Health, Vol. 21, No. 7, 2011, pp. 21-27. doi:10.1016/j.paed.2011.03.010

[6]   A. P. Simopoulos, “The Importance of the Ratio of Omega-6/Omega-3 Essential Fatty Acids,” Biomedical Pharmacotherapy, Vol. 56, No. 8, 2002, pp. 365-379. doi:10.1016/S0753-3322(02)00253-6

[7]   U. Risérus, “Fatty Acids and Insulin Sensitivity,” Current Opinion in Clinical Nutrition and Metabolic Care, Vol. 11, No. 2, 2008, pp. 100-105. doi:10.1097/MCO.0b013e3282f52708

[8]   L. Arab, “Biomarkers of Fat and Fatty Acid Intake,” The Journal of Nutrition, Vol. 133, No. 3, 2003, pp. 925S 932S.

[9]   S. D. Poppitt, P. Kilmartin, P. Butler and G. F. Keogh, “Assessment of Erythrocyte Phospholipid Fatty Acid Composition as a Biomarker for Dietary MUFA, PUFA or Saturated Fatty Acid Intake in a Controlled Cross-Over Intervention Trial,” Lipids in Health and Disease, Vol. 4, No. 30, 2005, pp. 30-39.

[10]   H. D. Orton, N. J. Szabo, M. Clare-Salzler and J. M. Nor ris, “Comparison between Omega-3 and Omega-6 Polyunsaturated Fatty Acid Intakes as Assessed by a Food Frequency Questionnaire and Erythrocyte Membrane Fatty Acid Composition in Young Children,” European Journal of Clinical Nutrition, Vol. 62, 2008, pp. 733-738. doi:10.1038/sj.ejcn.1602763

[11]   E. Warensjo, J. Sundstrom, L. Lind and B. Vessby, “Fac tor Analysis of Fatty Acids in Serum Lipids as a Measure of Dietary Fat Quality in Relation to the Metabolic Syn drome in Men,” The American Journal of Clinical Nu trition, Vol. 84, No. 2, 2006, pp. 442-448.

[12]   P. Risé, S. Eligini, S. Ghezzi, S. Colli and C. Galli, “Fatty Acid Composition of Plasma, Blood Cells and Whole Blood: Relevance for the Assessment of the Fatty Acid Status in Humans,” Prostaglandins, Leukotrienes and Essential Fatty Acids, Vol. 76, No. 6, 2007, pp. 363-369.

[13]   L. A. Baur, J. O’Connor, D. A. Pan, A. D. Kriketos and L. H. Storlien, “The Fatty Acid Composition of Skeletal Muscle Membrane Phospholipid: Its Relationship with the Type of Feeding and Plasma Glucose Levels in Young Children,” Metabolism, Vol. 47, No. 1, 1998, pp. 106-112. doi:10.1016/S0026-0495(98)90202-5

[14]   T. P. Novgorodtseva, Y. K. Karaman, N. V. Zhukova, E. G. Lobanova, M. V. Antonyuk and T. A. Kantur, “Com position of Fatty Acids in Plasma and Erythrocytes and Eicosanoids Level in Patients with Metabolic Syndrome,” Lipids in Health and Disease, Vol. 10, No. 82, 2011, pp. 82-86.

[15]   E. K. Kabagambe, M. Y. Tsai, P. N. Hopkins, J. M. Or dovas, J. M. Peacock, I. B. Borecki, et al., “Erythrocyte Fatty Acid Composition and the Metabolic Syndrome: A National Heart, Lung, and Blood Institute GOLDN Study,” Clinical Chemistry, Vol. 54, No. 1, 2007, pp. 154-162.

[16]   P. Mirmiran, S. Hosseinpour-Niazi, Z. Naderi, Z. Bahadoran, M. Sadeghi, F. Azizi, “Association between Interaction and Ratio of ω-3 and ω-6 Polyunsaturated Fatty Acid and the Metabolic Syndrome in Adults,” Nutrition, Vol. 28, No. 9, 2012, pp. 856-863. doi:10.1016/j.nut.2011.11.031

[17]   D. Mozaffarian, “Trans Fatty Acids—Effects on Sys temic Inflammation and Endothelial Function,” Atherosclerosis Supplements, Vol. 7, No. 2, 2006, pp. 29-32.

[18]   E. Christiansen, S. Schnider, B. Palmvig, E. Tauber Lassen and O. Pedersen, “Intake of a Diet High in Trans Monounsaturated Fatty Acids or Saturated Fatty Acids. Effects on Postpandrial Insulinemia and Glycemia in Obese Patients with NIDDM,” Diabetes Care, Vol. 20, No. 5, 1997, pp. 881-887. doi:10.2337/diacare.20.5.881

[19]   U. Risérus, P. Arner, K. Brismar and B. Vessby, “Treatment with Dietary Trans10cis12 Conjugated Linoleic Acid Causes Isomer-Specific Insulin Resistance in Obese Men with the Metabolic Syndrome,” Diabetes Care, Vol. 25, No. 9, 2002, pp. 1516-1521. doi:10.2337/diacare.25.9.1516

[20]   D. Mozaffarian, M. B. Katan, A. Ascherio, M. J. Stamp fer and W. C. Willett, “Trans Fatty Acids and Cardiovascular Disease,” The New England Journal of Medicine, Vol. 354, 2006, pp. 1601-1613. doi:10.1056/NEJMra054035

[21]   R. Micha and D. Mozaffarian, “Trans Fatty Acids: Effects on Cardiometabolic Health and Implications for Policy,” Prostaglandins, Leukotrienes and Essential Fatty Acids, Vol. 79, 2008, pp. 147-152. doi:10.1016/j.plefa.2008.09.008

[22]   J. Fritsche and H. Steinhart, “Analysis, Occurrence, and Physiological Properties of Trans Fatty Acids (TFA) with Particular Emphasis on Conjugated Linoleic Acid Isomers (CLA)—A Review,” European Journal of Lipid Science and Technology, Vol. 100, No. 6, 1998, pp. 190 210.

[23]   P. Salo, T. Seppanen-Laakso, I. Laakso, R. Seppanen, H. Niinikoski, J. Viikari and O. Simell, “Low Saturated Fat, Low-Cholesterol Diet in 5-Year-Old Children: Effect on Intake and Composition of Trans Fatty Acids and Other Fatty Acids in Serum Phospholipid Fraction—The STRIP Study,” The Journal of Pediatrics, Vol. 136, No. 1, 2000, pp. 46-52. doi:10.1016/S0022-3476(00)90048-9

[24]   J. E. Hunter, “Dietary Levels of Trans-Fatty Acids: Basis for Health Concerns and Industry Efforts to Limit Use,” Nutrition Research, Vol. 25, No. 5, 2005, pp. 499-513. doi:10.1016/j.nutres.2005.04.002

[25]   A. H. Lichtenstein, “Trans Fatty Acids and Blood Lipid Levels, Lp(A), Parameters of Cholesterol Metabolism, and Hemostatic Factors,” The Journal of Nutritional Biochemistry, Vol. 9, No. 9, 1998, pp. 244-248. doi:10.1016/S0955-2863(98)00016-3

[26]   F. Shahidi and S. P. J. N. Senanayake, “Fatty Acids,” In: K. Heggenhougen, Ed., International Encyclopedia of Public Health, Academic Press, San Diego, pp. 594-603.

[27]   R. P. Mensink and M. B. Katan. “Effect of Dietary Trans Fatty Acids on High-Density and Low-Density Lipopro tein Cholesterol Levels in Healthy Subjects,” The New England Journal of Medicine, Vol. 323, 1990, pp. 439-445. doi:10.1056/NEJM199008163230703

[28]   J. T. Judd, B. A. Clevidence, R. A. Muesing, J. Wittes, M. E. Sunkin and J. J. Podczasy, “Dietary Trans-Fatty Acids: Effects on Plasma Lipids and Lipoproteins of Healthy Men And Women,” The American Journal of Clinical Nutrition, Vol. 59, 1994, pp. 861-868.

[29]   I. Ramírez-Silva, S. Villalpando, J. E. Moreno-Saracho and D. Bernal-Medina, “Fatty Acids Intake in the Mexican Population. Results of the National Nutrition Survey 2006,” Nutrition & Metabolism, Vol. 8, No. 33, 2011.

[30]   J. R. Fernández, D. T. Redden, A. Petrobelli and D. B. Allison, “Waist Circumference Percentiles in Nationally Representative Samples of African-American, European American, and Mexican-American Children and Adoles cents,” The Journal of Pediatrics, Vol. 145, 2004, pp. 439-444.

[31]   National High Blood Pressure Education Program Working Group on High Blood Pressure in Children and Adolescents, “The Fourth Report on the Diagnosis, Evaluation, and Treatment of High Blood Pressure in Children and Adolescents,” Pediatrics, Vol. 114, No. 2, 2004, pp. 555-576.

[32]   S. Villalpando, I. Ramírez-Silva, D. Bernal-Medina and V. De la Cruz-Góngora, “Grasas, Dieta y Salud: Tablas de Composición de ácidos Grados de Alimentos Frecuentes en la Dieta Mexicana,” Instituto Nacional de Salud Pública, México City, 2007.

[33]   P. Koh-Banerjee, N. F. Chu, D. Spiegelman, B. Rosner, G. Colditz, W. Willet, et al., “Prospective Study of the As sociation of Changes in Dietary Intake, Physical Activity, Alcohol Consumption, and Smoking with 9-y Gain in Waist Circumference Among 16587 US Men,” The American Journal of Clinical Nutrition, Vol. 78, 2003, pp. 719-727.

[34]   M. Straczkowski, I. Kowalska, A. Nikolajuk, S. Dzienis Straczkowska, I. Kinalska, M. Baranowski, et al., “Rela tionship between Insulin Sensitivity and Sphingomyelin Signaling Pathway in Human Skeletal Muscle,” Diabetes, Vol. 53, 2004, pp. 1215-1221. doi:10.2337/diabetes.53.5.1215

[35]   J. Górski, A. Dobrzyn and M. Zendzian-Piotrowska, “The Sphingomyelin-Signalig Pathway in Skeetal Muscles and Its Role in Regulation of Glucose Uptake,” Annals of the New York Academy of Sciences, Vol. 967, 2002, pp. 236-248. doi:10.1111/j.1749-6632.2002.tb04279.x

[36]   V. Jakobik, I. Burus and T. Decsi, “Fatty Acid Composition of Erythrocyte Membrane Lipids in Healthy Subjects from Birth to Young Adulthood,” European Journal of Pediatrics, Vol. 168, 2009, pp. 141-147. doi:10.1007/s00431-008-0719-9

[37]   T. Burrows, C. E. Collins and M. L. Garg, “Omega-3 Index, Obesity and Insulin Resistance in Children,” International Journal of Pediatric Obesity, Vol. 6, 2011, pp. e532-e539.