FNS  Vol.4 No.9 , September 2013
Evaluation of Sensory and in Vitro Cardio Protective Properties of Sardine (Sardina pilchardus): The Effect of Grilling and Brining
Abstract: The aim of this study was to investigate the effect of grilling and brining on the sensory properties, the fillet fatty acid composition and the cardio-protective activity of sardine (Sardina pilchardus), studying the in vitro activity against Platelet-Activating-Factor (PAF) induced platelet aggregation. Sensory evaluation of grilled and brined sardine showed that grilled sardine had higher scores for the attributes: grilled fish, marine and fresh fish whereas brined sardine had higher scores for the attributes: salty, iodine, oily and bitter. Grilled sardine exhibited significantly increased fillet fatty acid content while the brined fish sample significantly decreased fatty acid levels. Polar lipids of all specimens (raw, grilled and brined) showed strong inhibitory activity against PAF action indicating that grilling and brining have not diminished the cardio-protective properties of sardine.
Cite this paper: C. Nasopoulou, E. Psani, E. Sioriki, C. Demopoulos and I. Zabetakis, "Evaluation of Sensory and in Vitro Cardio Protective Properties of Sardine (Sardina pilchardus): The Effect of Grilling and Brining," Food and Nutrition Sciences, Vol. 4 No. 9, 2013, pp. 940-949. doi: 10.4236/fns.2013.49122.

[1]   F. Médale, F. Lefèvre and G. Corraze, “Qualité Nutrition nelle et Diététique des Poissons: Constituants de la Chair et Facteurs de Variations,” Cahiers de Nutrition et de Diététique, Vol. 38, No. 1, 2003, pp. 37-44.

[2]   K. Gebauer, T. L. Psota, W. S. Harris and P. M. Kris Etherton, “ω-3 Fatty Acid Dietary Recommendations and Food Sources to Achieve Essentiality and Cardiovascular Benefits,” American Journal of Clinical Nutrition, Vol. 83, No. Suppl 6, 2006, pp. 1526-1535.

[3]   D. Bhatnagar and P. N. Durrington, “Omega-3 Fatty Ac ids: Their Role in the Prevention and Treatment of Athe rosclerosis Related Risk Factors and Complications,” In ternational Journal of Clinical Practice, Vol. 57, No. 4, 2003, pp. 305-314.

[4]   T. Nomikos, H. C. Karantonis, C. Skarvelis, C. A. Demo poulos and I. Zabetakis, “Antiatherogenic Properties of Lipid Fractions Obtained from Raw and Fried Fish Sam ples,” Food Chemistry, Vol. 96, No. 1, 2006, pp. 29-35. doi:10.1016/j.foodchem.2005.01.060

[5]   C. Nasopoulou, H. C. Karantonis, D. N. Perrea, S. E. Theocharis, D. G. Iliopoulos, C. A. Demopoulos and I. Zabetakis, “In Vivo Anti-Atherogenic Properties of Cul tured Gilthead Sea Bream (Sparus aurata) Polar Lipid Extracts in Hypercholesterolaemic Rabbits,” Food Chem istry, Vol. 120, No. 3, 2010, pp. 831-836. doi:10.1016/j.foodchem.2009.11.023

[6]   C. A. Demopoulos, R. N. Pinckard and D. J. Hanahan, “Platelet Activating Factor. Evidence for 1-O-alkyl-2-ace tyl-sn-glyceryl-3-phosphoryl-choline as the Active Com ponent (a New Class of Lipid Chemicalmediators),” Jour nal of Biological Chemistry, Vol. 254, No. 19, 1979, pp. 9355-9358.

[7]   C. A. Demopoulos, H. C. Karantonis and S. Antonopou lou, “Platelet Activating Factor—A Molecular Link be tween Atherosclerosis Theories,” European Journal of Lipid Science and Technology, Vol. 105, No. 11, 2003, pp. 705-716. doi:10.1002/ejlt.200300845

[8]   G. Subbanagounder, N. Leitinger, P. T. Shih, K. F. Faull and J. A. Berliner, “Evidence That Phospholipid Oxida tion Products and/or Platelet-Activating Factor Play an Important Role in Early Atherogenesis: in Vitro and in Vivo Inhibition by WEB 2086,” Circulation Research, Vol. 85, No. 4, 1999, pp. 311-318. doi:10.1161/01.RES.85.4.311

[9]   S. Antonopoulou, T. Nomikos, H. C. Karantonis, E. Fra gopoulou and C. Demopoulos, “PAF, a Potent Lipid Me diator,” In: A. D. Tselepis, Ed., Bioactive Phospholipids. Role in Inflammation and Atherosclerosis, Transworld Research Network, Kerala, 2008, pp. 85-134.

[10]   A. Bognar, “Comparative Study of Frying to Other Cook ing Techniques. Influence on the Nutritive Value,” Gra sas Aceites, Vol. 49, No. 3-4, 1998, pp. 250-260. doi:10.3989/gya.1998.v49.i3-4.746

[11]   S. Bellagha, A. Sahli, A. Farhat, N. Kechaou and A. Glenza, “Studies on Salting and Drying of Sardine (Sar dinella aurita): Experimental Kinetics and Modeling,” Journal of Food Engineering, Vol. 78, No. 3, 2007, pp. 947-952. doi:10.1016/j.jfoodeng.2005.12.008

[12]   E. G. Bligh and W. J. Dyer, “A Rapid Method of Total Lipid Extraction and Purification,” Canadian Journal of Biochemistry and Physiology, Vol. 37, No. 8, 1959, pp. 911-917. doi:10.1139/o59-099

[13]   D. S. Galanos and V. M. Kapoulas, “Isolation of Polar Lipids from Triglyceride Mixtures,” Journal Lipid Re search, Vol. 3, 1962, pp. 134-137.

[14]   C. Nasopoulou, G. Stamatakis, C. A. Demopoulos and I. Zabetakis, “Effects of Olive Pomace and Olive Pomace Oil on Growth Performance, Fatty Acid Composition and Cardio Protective Properties of Gilthead Sea Bream (Spa rus aurata) and Sea Bass (Dicentrarchus labrax),” Food Chemistry, Vol. 129, No. 3, 2011, pp. 1108-1113. doi:10.1016/j.foodchem.2011.05.086

[15]   C. Nasopoulou, T. Nomikos, C. A. Demopoulos and I. Zabetakis, “Comparison of Antiatherogenic Properties of Lipids Obtained from Wild and Cultured Sea Bass (Dicentrarchus labrax) and Gilthead Sea Bream (Sparus aurata),” Food Chemistry, Vol. 100, No. 2, 2007, pp. 560-567.

[16]   SO 10399, Sensory Analysis—Methodology—Duo-Trio Test, 2004.

[17]   ISO 4120, Sensory Analysis—Methodology—Triangle Test, 2004.

[18]   ISO 8587, Sensory Analysis—Methodology—Ranking, 2006.

[19]   C. M. Meilgaard, G. V. Civille and B. T. Carr, “Sensory Evaluation Techniques,” 3rd Edition, CRC Press, London, 1999.

[20]   M. J. Macfie, N. Bratchell, K. Greenhoff and L. V. Vallis, “Designs to Balance the Effect of Order of Presentation and First-Order Carry-Over Effects in Hall Tests,” Jour nal of Sensory Studies, Vol. 4, No. 2, 1989, pp. 129-148. doi:10.1111/j.1745-459X.1989.tb00463.x

[21]   A. Zotos, A. Kotaras and E. Mikras, “Effect of Baking of Sardine (Sardina pilchardus) and Frying of Anchovy (En graulis encrasicholus) in Olive Oil and Sunflower Oil on Their Quality,” Food Science and Technology Interna tional, Vol. 19, No. 1, 2013, pp. 11-23. doi:10.1177/1082013212442179

[22]   M. T. Garcia-Arias, E. Alvarez Pontes, M. C. Garcia Linares, M. C. Garcia-Fernande and F. J. Sanchez-Muniz, “Cooking-Freezing-Reheating (CFR) of Sardine (Sardina pilchardus) Fillets. Effect of Different Cooking and Re heating Procedures on the Proximate and Fatty Acid Compositions,” Food Chemistry, Vol. 83, No. 3, 2003, pp. 349-356. doi:10.1016/S0308-8146(03)00095-5

[23]   K. Bouderoua, J. Mourot, F. Benmehdi-Tabet-Aoull and G. Selselet-Attou, “The Effects of Season and Site of Catch on Morphometric Characteristics, Mineral Content, and Fatty Acids of Sardines (Sardina pilchardus) Caught on the Algerian Coast,” Journal of Aquatic Food Product Technology, Vol. 20, No. 4, 2011, pp. 412-420. doi:10.1080/10498850.2011.577272

[24]   J. E. Kinsella, B. Lokesh and R. A. Stone, “Dietary ω-3 Polyunsaturated Fatty Acids and Amelioration of Car diovascular Disease: Possible Mechanisms,” American Journal of Clinical Nutrition, Vol. 52, No. 1, 1990, pp. 1-28.

[25]   B. Ersoy, “Effects of Cooking Methods on the Proximate, Mineral and Fatty Acid Composition of European Eel (Anguilla anguilla),” International Journal of Food Sci ence and Technology, Vol. 46, No. 3, 2011, pp. 522-527. doi:10.1111/j.1365-2621.2010.02546.x

[26]   Y. Moradi, J. Bakar, A. A. Motalebi, S. H. Syed Mu hamad and Y. Che Man, “A Review on Fish Lipid: Com position and Changes during Cooking Methods,” Journal of Aquatic Food Product Technology, Vol. 20, No. 4, 2012, pp. 379-390. doi:10.1080/10498850.2011.576449

[27]   D. D. Nambudiry, “Lipid Oxidation in Fatty Fish: The Effect of Salt Content in Meat,” Journal of Food Science and Technology, Vol. 17, 1980, pp. 176-178.

[28]   J. Kanner and J. E. Kinsella, “Lipid Deterioration Initiat ed by Phagocytic Cells in Muscle Foods: Caroene De struction by a Myeloperoxidase-Hydrogenperoxidehalide System,” Journal of Agricultural and Food Chemistry, Vol. 31, No. 2, 1983, pp. 370-376. doi:10.1021/jf00116a047

[29]   N. Tsantila, H. C. Karantonis, D. N. Perrea, S. E. Theo charis, D. G. Iliopoulos, S. Antonopoulou and C. A. De mopoulos, “Antithrombotic and Antiatherosclerotic Prop erties of Olive Oil and Pomace Polar Extracts in Rabbits,” Mediators of Inflammation, Vol. 2007, No. 1, 2007, pp. 1-11. doi:10.1155/2007/36204

[30]   A. Tokumura, T. Sumida, M. Toujima, K. Kogure and K. Fukuzawa, “Platelet-Activating Factor (PAF)-Like Oxi dized Phospholipids: Relevance to Atherosclerosis,” Bio factors, Vol. 13, No. 1-4, 2000, pp. 29-33. doi:10.1002/biof.5520130106

[31]   R. Feliste, B. Perret, P. Braqueta and H. Chap, “Protec tive Effect of BN 52021, a Specific Antagonist of Plate let-Activating Factor (PAF Acether) against Diet-Induced Cholesteryl Ester Deposition in Rabbit Arta,” Athero sclerosis, Vol. 78, No. 2-3, 1989, pp. 151-158. doi:10.1016/0021-9150(89)90219-0

[32]   H. C. Karantonis, S. Antonopoulou, D. N. Perrea, D. P. Sokolis, S. E. Theocharis, N. Kavantzas, D. G. Iliopoulos and C. A. Demopoulos, “In Vivo Antiatherogenic Proper ties of Olive Oil and Its Constituent Lipid Classes in Hy perlipidemic Rabbits,” Nutrition, Metabolism and Car diovascular Diseases, Vol. 16, No. 3, 2006, pp. 174-185. doi:10.1016/j.numecd.2005.07.003

[33]   A. S. Wierzbicki, R. Poston and A. Ferro, “The Lipid and Non-Lipid Effects of Statins,” Pharmacology and Thera peutics, Vol. 99, No. 1, 2003, pp. 95-112. doi:10.1016/S0163-7258(03)00055-X