JBiSE  Vol.6 No.8 A , August 2013
Stimulation of decidua development by transplantation of endometrial stem cells
ABSTRACT
On all terms of pregnancy, insolvency of decidual reaction of endometrial cells is one of the reasons of miscarriages and fetal growth delay. The insufficient decidualization of endometrum leads to infertility in such pathologies, as Asherman’s syndrome and an endometrium atrophy. However, there are data on successful application of autologous bone marrow MSCs for Asherman’s syndrome treatment. The aim of this work was to assay the effect of endometrial mesenchymal stem cell (eMSC) transplantation for decidualization process in pseudopregnant rat. Our study showed that injection of human eMSC suspension into the uterine lumen of pseudopregnant rats facilitated more intensive development of decidua in comparison with phosphate buffed saline (PBS) injection in the control uterine horn. Histological analysis of decidua sections did not reveal any alterations in cell differentiation or tissue structure. In conclusion, we demonstrated for the first time that eMSC transplantation assists the development of all decidual tissue elements. It opens the possibility that eMSCs may be applied for cell therapy of infertility associated with decidualzation insufficiency.

Cite this paper
Domnina, A. , Zemelko, V. , Mikhailov, V. and Nikolsky, N. (2013) Stimulation of decidua development by transplantation of endometrial stem cells. Journal of Biomedical Science and Engineering, 6, 59-65. doi: 10.4236/jbise.2013.68A1006.
References
[1]   Kim, J.J., Taylor, H.S., Lu, Z, Ladhani, O., Hastings, J.M., Jackson, K.S., Wu, Y, Guo, S.W. and Fazleabas, A.T. (2007) Altered expression of HOXA10 in endometriosis: Potential role in decidualization. Molecular Human Reproduction, 13, 323-332. doi:10.1093/molehr/gam005

[2]   Gu, Y., Soares, M.J., Srivastava, R.K. and Gibori, G. (1994) Expression of decidual prolactin-related protein in the rat decidua. Endocrinology, 135, 1422-1427. doi:10.1210/en.135.4.1422

[3]   Rahkonen, L., Rutanen, E.M., Nuutila, M., Sainio, S., Saisto, T. and Paavonen, J. (2010) Elevated levels of decidual insulin-like growth factor binding protein-1 in cervical fluid in early and mid-pregnancy are associated with an increased risk of spontaneous preterm delivery. BJOG, 117, 701-710. doi:10.1111/j.1471-0528.2010.02551.x

[4]   Kearns, M. and Lala, P.K. (1982) Bone marrow origin of decidual cell precursors in the pseudopregnant mouse uterus. The Journal of Experimental Medicine, 155, 1537-1554. doi:10.1084/jem.155.5.1537

[5]   Bell, S.C. (1983) Decidualization: regional differentiation and associated function. Oxford Reviews of Reproductive Biology, 5, 220-271.

[6]   Brosens, J.J. and Gellersen, B. (2006) Death or survival—Progesterone-dependent cell fate decisions in the human endometrial stroma. Journal of Molecular Endocrinology, 36, 389-398. doi:10.1677/jme.1.02060

[7]   Finn, C.A., Pope, M.D. and Milligan, S.R. (1996) Relaxin and decidualization in mice: A reappraisal. Biology of Reproduction, 55, 1415-1418. doi:10.1095/biolreprod55.6.1415

[8]   Finn, C.A. and Keen, P.M. (1963) The induction of deciduomata in the rat. Journal of Embryology & Experimental Morphology, 11, 673-682.

[9]   Toshihiro, K., Amanda, R.G., Lea, A.R., Jennifer, K., HoChen, S.M., Khorshed, A., Pengli, B.M.A., Karim, R. and Michael, J.S. (2010) Subfertility linked to combined luteal insufficiency and uterine progesterone resistance. Endocrinology, 151, 4537-4550. doi:10.1210/en.2010-0440

[10]   Krehbiel, R.H. (1937) Cytological studies of the decidual reaction in the rat during early pregnancy and in the production of deciduomata. Physiological Zoology, 10, 212-234.

[11]   Mikhailov, V.M. (2003) Life cycle of decidual cells. International Review of Cytology, 227, 1-63. doi:10.1016/S0074-7696(03)01007-6

[12]   Taylor, H.S. (2004) Endometrial cells derived from donor stem cells in bone marrow transplant recipients. The Journal of the American Medical Association, 292, 81-85. doi:10.1001/jama.292.1.81

[13]   Schwab, K.E. and Gargett, C.E. (2007) Co expression of two perivascular cell markers isolates mesenchymal stem like cells from human endometrium. Human Reproduction, 22, 2903-2911. doi:10.1093/humrep/dem265

[14]   Cho, N.H., Park, Y.K., Kim, Y.T., Yang, H. and Kim, S.K. (2004) Lifetime expression of stem cell markers in the uterine endometrium. Fertility and Sterility, 81, 403-407. doi:10.1016/j.fertnstert.2003.07.015

[15]   Gargett, C.E. (2006) Identification and characterization of human endometrial stem/progenitor cells. Australian and New Zealand Journal of Obstetrics and Gynaecology, 46, 250-253. doi:10.1111/j.1479-828X.2006.00582.x

[16]   Meng, X., Ichim, T.E., Zhong, J., Rogers, A., Yin, Z., Jackson, J., Wang, H., Ge, W., Bogin, V., Chan, K.W., Thébaud, B. and Riordan, N.H. (2007) Endometrial regenerative cells: A novel stem cell population. Journal of Translational Medicine, 5, 57-66. doi:10.1186/1479-5876-5-57

[17]   Musina, R.A., Belyavsky, A.V., Tarusova, O.V., Solovyeva, E.V. and Sukhikh G.T. (2008) Endometrial mesenchymal stem cells obtained from menstrual blood, klet. tekhn. Biology and Medicine, 2, 110-114.

[18]   Patel, A.N., Park, E., Kuzman, M., Benetti, F., Silva, F.J. and Allickson, J.G. (2008) Multipotent menstrual blood stromal stem cells: Isolation, characterization, and differentiation. Cell Transplant, 17, 303-311. doi:10.3727/096368908784153922

[19]   Zemelko, V.I., Grinchuk, T.M., Domnina, A.P., Artzibasheva, I.V., Zenin, V.V., Kirsanov, A.A., Bichevaia, N.K., Korsak, V.S. and Nikolsky, N.N. (2012) Multipotent mesenchymal stem cells of desquamated endometrium: Isolation, characterization, and application as a feeder layer for maintenance of human embryonic stem cells. Cell and Tissue Biology, 6, 1-11. doi:10.1134/S1990519X12010129

[20]   Gargett, C.E. and Masuda, H. (2010) Adult stem cells in the endometrium. Molecular Human Reproduction, 16, 818-834. doi:10.1093/molehr/gaq061

[21]   Zemelko, V.I., Kozhukharova, I.B., Alekseenko, L.L., Domnina, A.P., Reshetnikova, G.F., Puzanov, M.V., Dmitrieva, R.I., Grinchuk, T.M., Nikolsky, N.N. and Anisimov, S.V. (2013) Neurogenic potential of human mesenchymal stem cells isolated from bone marrow, adipose tissue and endometrium: A comparative study. Cell and Tissue Biology, 7, 235-244. doi:10.1134/S1990519X13030140

[22]   Beer, A.E. and Billingham, R.E. (1974) Host responses to intrauterine tissue, cellular and fetal allografts. Journal of Reproduction & Fertility, 21, 59.

[23]   Csemiczky, G., Wramsby, H., Johannisson, E. and Landgren, B.M. (1998) Importance of endometrial quality in women with tubal infertility during a natural menstrual cycle for the outcome of IVF treatment. Journal of Assisted Reproduction and Genetics, 15, 55-61. doi:10.1007/BF02766825

[24]   Dmowski, W.P. and Greenblatt, R.B. (1969) Asherman’s syndrome and risk of placenta accreta. Obstetrics & Gynecology, 34, 288-299.

[25]   Nagori, C.B., Panchal, S.Y. and Patel, H. (2011) Endometrial regeneration using autologous adult stem cells followed by conception by in vitro fertilization in a patient of severe Asherman’s syndrome. Journal of Human Reproductive Sciences, 4, 43-48. doi:10.4103/0974-1208.82360

[26]   Domnina, A.P., Fridlyanskaya, I.I., Zemelko, V.I., Pugovkina, N.A., Kovaleva, Z.V., Zenin, V.V., Grinchuk, T.M. and Nikolsky, N.N. (2013) Mesenchymal stem cells from human endometrium do not undergo spontaneous transformation during long-term cultivation. Cell and Tissue Biology, 7, 221-226. doi:10.1134/S1990519X1303005X

 
 
Top