OJVM  Vol.3 No.5 , September 2013
Experimental Haematobiochemical Alterations in Broiler Chickens Fed with T-2 Toxin and Co-Infected with IBV
Abstract: The purpose of this experimental study was to evaluate and record the effects of T-2 toxicity alone and in association with IBV infection on haematobiochemical parameters. A total of 128 one-week-old chicks were divided into four groups of 32 birds each and were treated respectively with T-2 toxin alone, IBV alone, T-2 toxin and co-infected with IBV, and no treatment (control) for a period of 6 weeks. Haematologically, the birds treated with T-2 toxin developed anaemia as indicated by significant decrease in haemoglobin levels, total erythrocyte counts and packed cell volume values; leucopenia, lymphocytopenia heterophilia and thrombocytopenia. The IBV infected birds exhibited lymphocytophilia and heteropoenia; the degrees of severity of leucopenia, lymphocytopenia heterophilia and thrombocytopenia were more pronounced in T-2+IBV groups. The serum biochemistry revealed hypoproteinemia and hypoalbuminemia in all the treated groups consistently. Besides, hypoglobulinemia and increased levels of alanine aminotransferase in T-2+IBV, and increased levels of alkaline phosphatase in toxin group alone were recorded. The changes in biochemical parameters were more in magnitude in the combination treatment group and their severity increased with duration of treatment. It was concluded that T-2 toxin made the birds more susceptible to IBV infection.
Cite this paper: T. Yohannes, A. Sharma, S. Singh and V. Sumi, "Experimental Haematobiochemical Alterations in Broiler Chickens Fed with T-2 Toxin and Co-Infected with IBV," Open Journal of Veterinary Medicine, Vol. 3 No. 5, 2013, pp. 252-258. doi: 10.4236/ojvm.2013.35040.

[1]   H. S. Hussein and J. M. Brasel, “Toxicity, Metabolism, and Impact of Mycotoxins on Humans and Animals,” Toxicology, Vol. 167, No. 2, 2001, pp. 101-134. doi:10.1016/S0300-483X(01)00471-1

[2]   E. M. Binder, L. M. Tan, L. J. Chin, J. Handl and J. Richard, “Worldwide Occurrence of Mycotoxins in Commodities Feeds and Feed Ingredients,” Animal Feed Science Technology, Vol. 137, No. 3-4, 2007, pp. 265-282. doi:10.1016/j.anifeedsci.2007.06.005

[3]   A. E. Glenn, “Fusarium and Their Toxins: Mycology, Occurrence, Toxicity, Control and Economic Impact by Morgavi,” Animal Feed Science Technology, Vol. 137, No. 3, 2007, pp. 199-200.

[4]   A. E. Desjardins, “Fusarium Mycotoxins: Chemistry, Genetics, and Biology,” American Phytopathological Society, 2006, p. 260.

[5]   M. Sokolovic, V. Garaj-Vrhovac, S. Ramic and B. Simpraga, “Chicken Nucleated Blood Cells as a Cellular Model for Genotoxicity Testing Using the Comet Assay,” Food Chemical Toxicology, Vol. 45, No. 7, 2007, pp. 2165-2170. doi:10.1016/j.fct.2007.05.013

[6]   J. Shinozuka, G. Li, K. Uetsuka, H. Nakayama and K. Doi, “Process of the Development of T-2 Toxin-Induced Apoptosis in the Lymphoid Organs of Mice,” Experimental Animal, Vol. 46, 1997, pp. 117-126.

[7]   A. M. Murshedul, M. Nagase, T. Yoshizawa and N. Sakato, “Thymocyte Apoptosis by T-2 Toxin in Vivo in Mice Is Independent of Fas/Fas Ligand System,” Bioscience and Biotechnology and Biochemistry, Vol. 64, No. 1, 2000, pp. 210-213. doi:10.1271/bbb.64.210

[8]   A. Poapolathep, R. Ohtsuka, W. Kiatipattanasakul, N. Ishigami, H. Nakayama and K. Doi, “Nivalenol-Induced Apoptosis in Thymus, Spleen and Peyer’s Patches of Mice,” Experimental Toxicological Pathology, Vol. 53, No. 6, 2002, pp. 441-446. doi:10.1078/0940-2993-00211

[9]   G. M. Meissonnier, J. Laffitte, I. Raymond, E. Benoit, A. M. Cossalter, P. Pinton, G. Bertin, I. P. Oswald and P. Galtier, “Subclinical Doses of T-2 Toxin Impair Acquired Immune Response and Liver Cytochrome P450 in Pigs,” Toxicology, Vol. 247, No. 1, 2008, pp. 46-54. doi:10.1016/j.tox.2008.02.003

[10]   P. Villegas, “Viral Diagnosis of Respiratory System,” Poultry Science, Vol. 77, 1998, pp. 1143-1175.

[11]   D. Cavanagh and S. A. Naqi, “Infectious Bronchitis,” In: H. J. Barnes, J. R. Glisson, A. M. Fadly, L. R. McDougald, D. E. Swayne and Y. M. Saif, Eds., Diseases of Poultry, 11th Edition, Iowa State University Press, Ames, 2003, pp. 101-119.

[12]   I. P. Oswald, D. E. Martin, S. Bouhet, P. Pinton, I. Taranu and F. Accensi, “Immunological Risk of Mycotoxins for Domestic Animals,” Food Additives and Contaminants, Vol. 22, No. 4, 2005, pp. 354-360. doi:10.1080/02652030500058320

[13]   AOAC, “Official Method of Analysis,” 16th Edition, Association of Official Analytical Chemists, Washington DC, 1995.

[14]   L. J. Reed and H. Muench, “A Simple Method of Estimating 50% End-Point,” American Journal of Hygiene, Vol. 27, 1983, p. 493.

[15]   N. C. Jain, “Schalm’s Veterinary Haematology,” 4th Edition, Lea Febiger, Philadelphia, 1986.

[16]   V. V. Pande, N. V. Kurkure and A. Bhandarkar, “Effects of T-2 Toxin on Growth, Performance and Haematobiochemical Alterations in Briores,” Indian Journal of Experimental Biology, Vol. 44, 2006, pp. 86-88.

[17]   P. Krishnamoorthy, S. Vairamuthu, C. Balachandra and B. Muralimanohar, “Chlorpyriphos and T-2 Toxin Induced Haemato-Biochemical Alterations in Broiler Chicken,” International Journal of Poultry Science, Vol. 5, No. 2, 2006, pp. 173-177. doi:10.3923/ijps.2006.173.177

[18]   F. J. Hoerr, W. W. Carlton, B. Yagen and A. Z. Joffe, “Mycotoxicosis Caused by Either T-2 Toxin or Diacetoxyscirpenol in the Diet of Broiler Chickens,” Fundamental Applied Toxicology, Vol. 2, 1982, pp. 121-124. doi:10.1016/S0272-0590(82)80092-4

[19]   T. A. Yohannes, “Clinicopathological, Pathological and Immunological Studies on Experimental T-2 Mycotoxicosis and IBV Infection in Broiler Chicken,” M.V.Sc. Thesis, Indian Veterinary Research Institute, India, 2010.

[20]   W. E. Huff, J. A. Doerr and P. B. Hamilton, “Decreased Glycogen Mobilization during Ochratoxicosis in Broiler Chickens,” Applied and Evironmental Microbiology, Vol. 37, No. 1, 1979, pp. 122-126.

[21]   F. J. Hoerr, “Mycotoxicoses: Diseases of Poultry,” 11th Edition, Iowa State University Press, Ames, Iowa, 2003, pp. 1103-1132.

[22]   A. M. Shareef, “Some Immunosuppressive Effect of T-2 Toxin in Broiler Chicks,” Iraqi Journal of Veterinary Sciences, Vol. 19, No. 2, 2005, pp. 99-108.

[23]   S. R. Chowdhury, T. K. Smithm, H. J. Boermans and B. Woodward, “Effects of Feed-Borne Fusarium Mycotoxins on Hematology and Immunology of Laying Hens,” Poultry Science, Vol. 84, 2005, pp. 1841-1850.

[24]   U. K. Mishra, P. K. Dwarakanath, S. P. Agrawal and M. I. Hossain, “Effect of T-2 Toxin on Haemostatic Profile in Growing Chickens,” Indian Veterinary Journal, Vol. 73, No. 11, 1996, pp. 1133-1137.

[25]   T. H. Nataraja, H. D. N. Swamy, S. K. Vijayasarathi, G. C. Prakash and B. S. Kumar, “Biochemical Alterations in Combined Mycotoxicosis of Broiler Chicks,” Indian Veterinary Journal, Vol. 81, No. 3, 2004, pp. 264-266.

[26]   R. Madheswaran, C. Balachandran and B. M. Manohar, “Pathological Effects of Feeding Aflatoxin and T-2 Toxin in Japanese Quail,” Indian Journal of Veterinary Pathology, Vol. 29, No. 1, 2005, pp. 23-26.

[27]   M. Yegani, T. K. Smith, S. Leeson and H. J. Boermanst, “Effect of Feeding Grains Naturally Contaminated with Fusarium Mycotoxins on Performance and Metabolism of Broiler Breeders,” Poultry Science, Vol. 85, 2006, pp. 1541-1549.

[28]   E. Cundliffe, M. Cannon and J. Davies, “Mechanism of Inhibition of Eukaryotic Protein Synthesis by Trichothecene Fungal Toxins,” Proceedings of National Academy of Sciences, Vol. 71, 1974, pp. 30-40. doi:10.1073/pnas.71.1.30

[29]   D. E. Corrier, “Mycotoxicosis: Mechanisms of Immunosuppression,” Veerinary Immunology and Immunopathology, Vol. 30, No. 1, 1991, pp. 73-87.

[30]   S. Leeson, G. Diaz and J. D. Summers, “Poultry Metabolic Disorders and Mycotoxins,” University Books, Ontario, 1995.

[31]   K. L. Arvind, V. S. Patil, G. Devegowda, B. Umakantha and S. P. Ganpule, “Efficacy of Esterified Glucomannan to Counteract Mycotoxicosis in Naturally Contaminated Feed on Performance and Serum Biochemical and Hematological Parameters in Broilers,” Poultry Science, Vol. 82, No. 4, 2003, pp. 571-576.