Health  Vol.2 No.12 , December 2010
Effects of fullerene nanowhiskers on cytotoxicity and gene expression
ABSTRACT
With recent developments in nanoscience and nanotechnology, carbon-based nanomaterials, such as carbon nanotubes or Fullerene nanowhiskers (FNWs), are attracting a great deal of interest. However, nanomaterials have novel properties that may cause safety problems in biological systems. Although the toxic effects of multiwall carbon nanotubes (MWCNTs) in vitro and in vivo have been well described, the effect of FNWs on cells remains unclear. In the present study, we analyzed the gene expression and cytotoxicity of FNWs-treated cells. The results of cell viabil-ity and gene expression analysis indicate that FNWs has a relatively smaller ability to induce cellular gene expression as compared with MWCNTs or titania nanoparticles. Our results suggest that FNWs have weak cytotoxic effects as compared to the effects of MWCNTs and tita-nia nanoparticles.

Cite this paper
nullOkuda-Shimazaki, J. , Nudejima, S. , Takaku, S. , Kanehira, K. , Sonezaki, S. and Taniguchi, A. (2010) Effects of fullerene nanowhiskers on cytotoxicity and gene expression. Health, 2, 1456-1459. doi: 10.4236/health.2010.212216.
References
[1]   [1] Nel, A., Xia, T., M?dler, L., Li, N. (2006) Toxic potential of materials at the nanolevel. Science, 311 (5761), 622- 627.

[2]   [2] Service, R.F. (2004) Nanotoxicology. Nanotechnology grows up. Science, 304 (5678), 1732-1734.

[3]   [3] Oberd?rster, G., Oberd?rster, E., Oberd?rster, J. (2005) Nanotoxicology: An emerging discipline evolving from studies of ultrafine particles. Environ. Health Perspect. 113(7), 823-839.

[4]   [4] Poland, C.A., Duffin, R., Kinloch, I., et al. (2008) Carbon nanotubes introduced into the abdominal cavity of mice show asbestos-like pathogenicity in a pilot study. Nat. Nanotechnol., 3 (7), 423-428.

[5]   [5] Takagi, A., Hirose, A., Nishimura, T., et al. (2008) Induction of mesothelioma in p53+/- mouse by intraperitoneal application of multi-wall carbon nanotube. J. Toxicol. Sci., 33 (1), 105-116.

[6]   [6] Okuda-Shimazaki, J., Takaku, S., Kanehira, K., et al. (2010) Aggregate Particle Size Effects of Titanium Dioxide Nanoparticles on the Gene Expression. Int. J. Mol. Sci., 11, 2383-2392.

[7]   [7] Patlolla, A., Patlolla, B., Tchounwou, P. (2010) Evaluation of cell viability, DNA damage, and cell death in normal human dermal fibroblast cells induced by functionalized multiwalled carbon nanotube. Mol Cell Biochem., 338 (1-2), 225-232.

[8]   Jos, A., Pichardo, S., Puerto, M., et al. (2009) Cameán AM. Cytotoxicity of carboxylic acid functionalized single wall carbon nanotubes on the human intestinal cell line Caco-2. Toxicol In Vitro. 23 (8), 1491-1496.

[9]   [8] Lin, C., Fugetsu, B., Su, Y., Watari, F. (2009) Studies on toxicity of multi-walled carbon nanotubes on Arabidopsis T87 suspension cells. J Hazard Mater. 170 (2-3), 578-583.

[10]   [9] Tong, H., McGee, JK., Saxena, R.K., et al. (2009) Influence of acid functionalization on the cardiopulmonary toxicity of carbon nanotubes and carbon black particles in mice. Toxicol Appl Pharmacol., 239 (3), 224-232.

[11]   [10] Belyanskaya, L., Weigel, S., Hirsch, C., et al. (2009) Effects of carbon nanotubes on primary neurons and glial cells. Neurotoxicology. 30 (4), 702-711.

[12]   [11] Miyazawa, K., Kuwasaki, Y., Obayashi, A., Kuwabara, M. (2002) C60 nanowhiskers formed by the liquid-liquid interfacial precipitation method. J. Mater. Res., 17 (1), 83-88.

[13]   [12] Miyazawa, K., Cha, S., Ringor, C., et al. (2008) Synthesis of fullerene nanotubes and microtubes for materials storage, delivery and recovery. Nano, 3, 335-339.

[14]   [13] Nudejima S., Miyazawa K., Okuda J., et al. (2009) Observation of phagocytosis of fullerene nanowhiskers by PMA-treated THP-1 cells. J. Physics, 159, 1-6.

[15]   [14] Okuda-Shimazaki, J., Yamamoto, A., Kuroda, D., et al. (2007) The effect of metal materials on heat shock protein 70B’ gene expression. Open Biotechnol. J. 1, 14-17.

[16]   [15] Nudejima S, Miyazawa K, Okuda-Shimazaki J et al. (2010) Biodegradation of C60 Fullerene Nanowhiskers by Macrophage-like Cells. ADVANCES IN BIOMEDICAL RESEARCH, pp.89-94

 
 
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