Many cases of toxicity and mortality either due to accidental or deliberate ingestion of hair dyes were reported in Egypt, Sudan, Israel, Morocco, Saudi Arabia, India and Tunisia. In the field of forensic analytical toxicology, the identification and quantification of chemicals are essential in deaths related to intoxication. The aim of the present study is to conduct chemico-analytical characterization of para-phenylenediamine (PPD) based hair dye of (Black stone hair dye, SHD) currently used for dying hear in Egypt, to confirm the identification of its components, that may be potentially toxic on oral or dermal exposure, this study uses comparison between different analytical methods. The applied analytical methods were inductively coupled plasma/MS (ICP/MS), witch used to determine the metal components of the dye, high performance liquid chromatography/MS (HPLC/MS) and gas chromatography/mass spectroscopy (GC/MS) were used for qualitative and quantitative analysis of PPD in the dye. A derivatization method was applied by transforming PPD (aromatic amines) into corresponding imine derivatives. This compound was prepared by condensation of 4-methylbenzeneamine with benzaldehyed to give N-benzylidene-4-methylbenzene-amine, which is a very stable internal standard. ICP/MS analysis revealed that most metal components of hair dye are Be, Na, Al, K, Ca, Sc, Ti, V, Cr, Mn, Fe, Cu, and Zr, where HPLC/MS analysis revealed that PPD is the main ingredient of SHD. Derivatization is necessary for accurate determination of PPD in hair dye by GC/MS method and this results could not be obtained by direct injection of dye (without derivatization).
 L. S. Cook, K. E. Malone, J. R. Daling, L. F. Voigt and N. S. Weiss, “Hair product use and the risk of breast cancer in young women,” Cancer Causes and Control, Vol. 10, No. 6, 1999, pp. 551-559. doi:10.1023/A:1008903126798
 C. Nagata, H. Shimizu, K. Hirashima, E. Kakishita, K. Fujimura, Y. Niho, M. Karasawa, S. Oguma, Y. Yoshiday and H. Mizoguchi, “Hair Dye Use and Occupational Exposure to Organic Solvents as Risk Factors for Myelodysplastic Syndrome,” Leukemia Research, Vol. 23, No. 1, 1999. pp. 57-62. doi:10.1016/S0145-2126(98)00135-0
 M. Gago-Dominguez, J. E. Castelao, J. M. Yuan, M. C. Yu and R. K. Ross, “Use of Permanent Hair Dyes and Bladder-Cancer Risk,” International Journal of Cancer, Vol. 91, No. 4, 2001, pp. 575-579. doi:10.1002/1097-0215(200002)9999:9999<::AID-IJC1092>3.0.CO,2-S
 M. Hashim, Y. O. Hamza, B. Yahia, F. M. Khogali and G. I. Sulieman, “Poisoning from Henna Dye and Para-Phenylenediamine Mixtures in Children in Khartoum,” Annals of Tropical Paediatrics, Vol. 12, No. 1, 1992, pp. 3-6.
 Y. Ikarashi, K. Ohno, J. Momma, T. Tsuchiya, A. Nakamura, “Assessment of Contact Sensitivity of Four Thiourea Rubber Accelerators: Comparison of Two Mouse Lymph Node Assays with the Guinea Pig Maximization Test,” Food and Chemical Toxicology, Vol. 32, No. 11, 1994, pp. 1067-1072. doi:10.1016/0278-6915(94)90148-1
 V. Andrisano, R. Gotti, A. M. Di Pietra and V. Carrini, “HPLC Analysis of Oxidation Hair Dyes in Permanent Hair Colorants,” Journal of Liquid Chromatography, Vol. 17, No. 13, 1994, pp. 2919-2937. doi:10.1080/10826079408013510
 N. Suresh and C. Rastogi, “A Method for the Analysis of Intermediates of Oxidative Hair Dyes in Cosmetic Products,” Journal of Separation Science, Vol. 24, No. 3, 2001, pp. 173-178. doi:10.1002/1615-9314(20010301)24:3<173::AID-JSSC173>3.0.CO,2-L
 N. Tanada, M. Kaegura, K. Hara, Y. Hieda, M. Takamoto and S. Kashimura, “Demonstration of Oxidation Dyes on Human Hair,” Forensic Science International, Vol. 64, No. 1, 1994, p. 1-8. doi:10.1016/0379-0738(94)90237-2
 Y. Kawamura, M. Miura, T. Sugita and T. Yamada, “Residue and Release of Antioxidants and Ultraviolet Stabilizers in Polyethylene Products in Contact with Foodstuffs,” Journal of the Food Hygienic Society of Japan, Vol. 38, No. 1, 1997, pp. 27-33. doi:10.3358/shokueishi.38.27
 M. L. Di Gioia, A. Le Pera, A. Liguori, A. Leggio, C. Siciliano and G. Sindona, “Highly Stereoselective Synthesis of Optically Pure C-Aryl Imines from α-L-Amino Acid Methyl Esters.,” Synthetic Communication, Vol. 33, No. 24, 2003, pp. 4331-4338.
 F. Denizot and R. Lang, “Rapid Colorimetric Assay for Cell Growth and Survival. Modifications to the TetraZolium Dye Procedure Giving Improved Sensitivity and Reliability,” Journal of Immunological Methods, Vol. 89, No. 2, 1986, pp. 271-277.
 K. Modha, J. P. Whiteside and R. E. Spier, “The Determination of Cellular Viability of Hybridoma Cells in Microtitre Plates: A Colorimetric Assay Based on Neutral Red,” Cytotechnology, Vol. 13, No. 3, 1993, pp. 227-232. doi:10.1007/BF00749819
 Y. Ikarashi and M. Kaniwa, “Determination of p-Phenylenediamine and Related Antioxidants in Rubber Boots by High Performance Liquid Chromatography. Development of an Analylical Method for N-(1-Methylheptyl)N’-Phenyl-p-Phenylenediamine,” Journal of Health Science, Vol. 46, No. 6, 2000, pp. 467-473.
 J. E. Coligan, A. M. Kruisbeek, D. H. Margulies, E. M. Shevach and W. Stober, “Measurement of Tumor Necrosis Factor A and B,” In: R. Coico, Ed., Current Protocols in Immunology, John Wiley and Sons, Inc., New York, 1996.
 H. Kataoka, “Derivatization Reactions for the Determination of Amines by Gas Chromatography and Their Applications in Environmental Analysis,” Journal of Chromatography A, Vol. 733, No. 1-2, 1996, pp. 19-34.
 E. H. Soufleros, E. Bouloumpasi, A. Zotou and Z. Loukou, “Determination of Biogenic Amines in Greek Wines by HPLC and Ultraviolet Detection after Dansylation and Examination of Factors Affecting Their Presence and Concentration,” Food Chemistry, Vol. 101, No. 2, 2007, pp. 704-716.
 R. Ghosh, N. Nadiminty, J. E. Fitzpatrick, W. L. Alworth, T. J. Slaga and A. P. Kumar, “Eugenol Causes Melanoma Growth Suppression through Inhibition of E2F1 Transcriptional Activity,” The Journal of Biological Chemistry, Vol. 280, No. 7, 2005, pp. 5812-5819. doi:10.1074/jbc.M411429200