JACEN  Vol.2 No.3 , August 2013
Biphenyl- and carvone-induced protein expression patterns in Rhodococcus sp. ACS
Abstract: Protein expression patterns in the polychlorinated biphenyl (PCB)-degrading Rhodococcussp. strain ACS were examined following growth on two substrates capable of inducing the enantioselective biotransformation of PCBs via different degradation pathways. Eleven inducible proteins were identified by SDS-PAGE and characterized by LC-MS/MS. Four of the peptides, a spore coat protein, an extracellular serine protease, a spoVP, and a molecular chaperonin from Bacillus subtilis, were identified as being unique to biphenyl-induced cells, whereas anextracellular serine protease from B. subtilis was identified as being unique to carvone-induced cells. None of the peptides identified had sequences that corresponded to known dioxygenases or other PCB-degrading enzymes of this Gram- positive bacterium, suggesting that the identified induced proteins may be involved in either PCB degradation or adaptive responses that protect cells from toxicity.
Cite this paper: J. Kim, "Biphenyl- and carvone-induced protein expression patterns in Rhodococcus sp. ACS," Journal of Agricultural Chemistry and Environment, Vol. 2 No. 3, 2013, pp. 65-73. doi: 10.4236/jacen.2013.23010.

[1]   Holoubek, I. (2001) Polychlorinated biphenyl (PCB) contaminated sites worldwide. In: Robertson, L.W. and Hansen, L.G.. Ed., PCBs: Recent Advances in Environmental Toxicology and Health Effects, University of Kentucky Press, Lexington, 17-26.

[2]   Park, J.S., Linderholm, L., Charles, M.J., Athanasiadou, M., Petrik, J., Kocan, A., Drobna, B., Trnovec, T., Bergman, A. and Hertz-Picciotto, I. (2007) Polychlorinated biphenyls and their hydroxylated metabolites (OH-PCBs) in pregnant women from eastern Slovakia. Environmental Health Perspectives, 115, 20-27. doi:10.1289/ehp.8913

[3]   Rezek, J., Macek, T., Mackova, M., Triska, J. and Ruzickova, K. (2008) Hydroxy-PCBs, methoxy-PCBs and hydroxyl-methoxy-PCBs: Metabolites of polychlorinated biphenyls formed in vitro by tobacco cells. Environmental Science & Technology, 42, 5746-5751. doi:10.1021/es800445h

[4]   Zorádová-Murínová, S., Dudá?o-vá, H., Luká?ová, L., Certík, M., Silharová, K., Vrana, B. and Dercová, K. (2012) Adaptation mechanisms of bacteria during the degradation of polychlorinated biphenyls in the presence of natural and synthetic terpenes as potential degradation inducers. Applied Microbiology and Biotechnology, 94, 1375-1385. doi:10.1007/s00253-011-3763-8

[5]   Focht, D.D. (1995) Strategies for the improvement of aerobic metabolism of polychlorinated biphenyls. Current Opinion in Biotechnology, 6, 341-407. doi:10.1016/0958-1669(95)80057-3

[6]   Gilbert, E.S. and Crowley, D.E. (1997) Plant compounds that induce polychlorinated biphenyl biodegradation by Arthrobacter sp. strain B1B. Applied and Environmental Microbiology, 63, 1933-1938.

[7]   Gilbert, E.S. and Crowley, D.E. (1998) Repeated application of carvone-induced bacteria to enhance biodegradation of polychlorinated biphenyls in soil. Applied Microbiology and Biotechnology, 50, 489-494. doi:10.1007/s002530051325

[8]   Singer, A.C., Gilbert, E.S., Luepromchai, E. and Crowley, D.E. (2000) Bioremediation of polychlorinated biphenyl-contaminated soil using carvone and surfactant-grown bacteria. Applied Microbiology and Biotechnology, 54, 638-843. doi:10.1007/s002530000472

[9]   Singer, A.C., Wong, C.S. and Crowley, D.E. (2002) Differential enantioselective transformation of atropisomeric polychlorinated biphenyls by multiple bacterial strains with different inducing compounds. Applied and Environmental Microbiology, 68, 5756-5759. doi:10.1128/AEM.68.11.5756-5759.2002

[10]   Tandlich, R., Brezna, B. and Dercova, K. (2001) The effect of terpenes on the biodegradation of polychlorinated biphenyls by Pseudomonas stutzeri. Chemosphere, 44, 1547-1555. doi:10.1016/S0045-6535(00)00523-3

[11]   Kohler, H.-P.E., Kohler-Staub, D. and Focht, D.D. (1988) Cometabolism of polychlorinated biphenyls: Enhanced transformation of Aroclor 1254 by growing bacterial cells. Applied and Environmental Microbiology, 54, 1940-1945.

[12]   Singer, A.C., Jury, W., Luepromchai, E., Yahng, C.S. and Crowley, D.E. (2001) Contribution of earthworms to PCB bioremediation. Soil Biology & Biochemistry, 33, 765-776. doi:10.1016/S0038-0717(00)00224-8

[13]   Furukawa, K. and Arimura, N. (1987) Nucleotide sequence of the 2,3-dihydroxybiphenyl dioxygenase gene of Pseudomonas pseudoalcaligenes. Journal of Bacteriology, 169, 924-927.

[14]   Haddock, J.D. and Gibson, D.T. (1995) Purification and characterization of the oxygenase component of biphenyl 2,3-dioxygenase from Pseudomonas sp. strain LB400. Journal of Bacteriology, 177, 5834-5839.

[15]   Hurtubise, Y., Barriault, D., Powlowski, J. and Sylvestre, M. (1995) Purification and characterization of the Comamonas testosteroni B-356 biphenyl dioxygenase components. Journal of Bacteriology, 177, 6610-6618.

[16]   Leigh, M.B., Prouzova, P., Mackova, M., Macek, T., Nagle, D.P. and Fletcher, J.S. (2006) Polychlorinated biphenyl (PCB)-degrading bacteria associated with trees in a PCB-contaminated site. Applied and Environmental Microbiology, 72, 2331-2342. doi:10.1128/AEM.72.4.2331-2342.2006

[17]   Benvenuti, M., Briganti, F., Scozzafava, A., Golovleva, L., Travkin, V.M. and Mangani, S. (1999) Crystallization and preliminary crystallographic analysis of the hydroxyquinol 1,2-dioxygenase from Nocardioides simplex 3E: A novel dioxygenase involved in the biodegradation of polychlorinated aromatic compounds. Acta Crystallographica Section D: Biological Crystallography, D55, 901-903. doi:10.1107/S0907444998017715

[18]   Ohmori, T., Morita, H., Tanaka, M., Miyauchi, K., Kasai, D., Furukawa, K., Miyashita, K., Ogawa, N., Masai, E. and Fukuda, M. (2011) Development of a strain for efficient degradation of polychlorinated biphenyls by patchwork assembly of degradation pathways. Journal of Bioscience and Bioengineering, 111, 437-442. doi:10.1016/j.jbiosc.2010.12.002

[19]   Patrauchan, M.A., Miyazawa, D., LeBlanc, J.C., Aiga, C., Florizone, C., Dosanjh, M., Davies, J., Eltis, L.D. and Mohn, W.W. (2012) Proteomic analysis of survival of Rhodococcus jostii RHA1 during carbon starvation. Applied and Environmental Microbiology, 78, 6714-6725. doi:10.1128/AEM.01293-12

[20]   Chang, I.F., Szick-Miranda, K., Pan, S. and Bailey-Serres, J. (2005) Proteomic characterization of evolutionarily conserved and variable proteins of Arabidopsis cytosolic ribosomes. Plant Physiology, 137, 848-862. doi:10.1104/pp.104.053637

[21]   Perkins, D. N., Pappin, D.J.C., Creasy, D.M. and Cottrell, J.S. (1999) Probability-based protein identification by searching sequence databases using mass spectrometry data. Electrophoresis, 20, 3551-3567. doi:10.1002/(SICI)1522-2683(19991201)20:18<3551::AID-ELPS3551>3.0.CO;2-2

[22]   Lai, E.M., Phadke, N.D., Kachman, M.T., Giorno, R., Vazquez, S., Vazquez, J.A., Maddock, J.R. and Driks, A. (2003) Proteomic analysis of the spore coats of Bacillus subtilis and Bacillus anthracis. Journal of Bacteriology, 185, 1443-1454. doi:10.1128/JB.185.4.1443-1454.2003