The research is designed to study the
relationship of intake of fatty acids through fast food items, consumption
trends and related health issues. Fried food products most commonly consumed at
restaurants were selected. Food outlets of both branded and non-branded vendors
were included in the study for comparison. Total fat content, Fatty acids and
Fatty Acid Methyl Esters (FAME’s) in food samples were determined
experimentally using titrimetric and spectrophotometric methods. Fatty acids of
Stearic, Oleic and Myristic were extracted from each food sample using petroleum
ether as extracting solvent, followed by conversion to FAME’s by treating the
filtrate with methylamine, ethanol and sulphuric acid. A survey analysis using the
questionnaire as a tool was also conducted. The results showed variable concentration
in each sample. Total fat content was highest in French fries, followed by fish
and chicken. Among the fatty acids, Stearic acid was found higher in
concentration than other two fatty acids. The study concluded that the level of
saturated fatty acids was 0.4 g (7.6%), which was securely under the allowable
limit of daily value (on a 2000 calorie diet) of 3 g (15%).
Cite this paper
A. Wasti and U. Rafique, "Extraction, Methylation and Quantification of Fatty Acids in Fast Food Items and Its Health Implications," Advances in Chemical Engineering and Science
, Vol. 3 No. 3, 2013, pp. 1-5. doi: 10.4236/aces.2013.33A2001
 Z. Fang, Z. W. Chen, Z. Xian, S. Y. Rui, L. G. Juan and Y. J. Mao, “Determination of Free Fatty Acids by High Performance Liquid Chromatography with Fluorescence Detection and Identification with Mass Spectrometry,” Chinese Journal of Analytical Chemistry, Vol. 35, No. 4, 2007, pp. 489-494. doi:10.1016/S1872-2040(07)60045-0
 H. W. Lawson, “Standards for Fats and Oils,” The Publishing Company, Westport, 1985.
 L. M. Smith, A. J. Clifford, R. K. Creceling and C. L. Hamblin, “Lipid Content and Fatty Acid Profiles of Various Deep-Fat Fried Foods,” Journal of the American Oil Chemists Society, Vol. 62, No. 6, 1985, pp. 996-999.
 D. P. Houhoula, V. Oreopoulou and C. J. Tzia, “A Kinetic Study of Oil Deterioration during Frying and a Comparison with Heating,” Journal of the American Oil Chemists’ Society, Vol. 79, No. 2, 2002, pp. 133-137.
 H. Gloria and J. M. Aguilera, “Assessment of the Quality of Heated Oils by Differential Scanning Calorimetry,” Journal of Agricultural and Food Chemistry, Vol. 46, No. 4, 1998, pp. 1363-1368. doi:10.1021/jf9703664
 W. L. Clark and G. W. Serbia, “Safety Aspects of Frying Fats and Oils,” Food Technology, Vol. 45, No. 2, 1991, pp. 84-89.
 C. C. Akoh and D. B. Min, “Food Lipids: Chemistry, Nutrition and Biotechnology,” 2nd Edition, Marcel Dekker, New York, 2002.
 H.-Q. Wu, X.-L. Huang, X.-S. Lin, F. Huang, Z.-X. Zhu and Y.-F. Ma, “Gas Chromatographic Retention Time Rule and Mass Spectrometric Fragmentation Rule of Fatty Acids and Its Application in Food,” Chinese Journal of Analytical Chemistry, Vol. 35, No. 7, 2007, pp. 998-1003.
 US Department of Agriculture and Health and Human services, “Dietary Guidelines for Americans,” 2010.
 F. Anwar, M. Bhanger, S. Latif and M. Manzoor, “Lipid content and Fatty Acid Composition of Some Deep Fried and Fast Foods from Sindh,” Journal of Chemistry, Vol. 28, No. 4, 2006, pp. 374-379.
 P. Observer, “Weight Problem in Pakistan,” 2010.