Back
 FNS  Vol.4 No.8 , August 2013
Protective Effect of Gingo biloba Extract on Carbendazim-Induced Hepatotoxicity in Albino Rats
Abstract: Carbendazim is a broad spectrum carbamate fungicide used in the control of various fungal pathogens. The present work studied the effect of carbendazim on the liver of albino rats and the possible protective role of Ginko biloba extract (EGB). Liver of carbendazim-treated animals showed histopathological and histochemical alterations. The histopathological changes include hepatic tissue impairment, cytoplasmic vacuolization of the hepatocytes, and congestion of blood vessels, leucocytic infiltrations and fatty infiltration. Histochemical results showed reduction of carbohydrates and total proteins in hepatic tissues. Moreover, liver function enzymes (ALT, AST) were elevated in sera of carbendazim-treated animals. Coadministration of EGB with Carbendazim improved the hisological and histochemical changes observed in animals treated with carbendazim. In addition, EGB treatment leads to a significant decrease in ALT and AST. According to the present results, it is concluded that EGB can improve the hepatotoxicity of carbendazim and this effect may be attributed to antioxidant properties of Ginko biloba extract.
Cite this paper: F. Mahboub and H. Lamfon, "Protective Effect of Gingo biloba Extract on Carbendazim-Induced Hepatotoxicity in Albino Rats," Food and Nutrition Sciences, Vol. 4 No. 8, 2013, pp. 866-872. doi: 10.4236/fns.2013.48113.
References

[1]   W. J. Hayes and E. R. Laws, “Handbook of Pesticide Toxicology, Vol. 3, Classes of Pesticides,” Academic Press, New York, 1990.

[2]   H. H. Oruc, M. Cengiz and H. Beskaya, “Chronic Copper Toxicosis in Sheep Following the Use of Copper Sulfate as a Fungicide on Fruit Trees,” Journal of Veterinary Diagnostic Investigation, Vol. 21, No. 4, 2009, pp. 540-543. doi:10.1177/104063870902100420

[3]   R. S. Guitart, X. Manosa, G. Guerrero and R. Mateo, “Animal Poisonings: The 10-Year Experience of a Veterinary Analytical Toxicology Laboratory,” Veterinary and Human Toxicology, Vol. 41, No. 5, 1999, pp. 331-335.

[4]   F. Mortazavi and A. Jafari-Javid, “Acute Renal Failure due to Copper Sulphate Poisoning: A Case Report,” Iranian Journal of Pediatrics, Vol. 19, No. 1, 2009, pp. 7578.

[5]   R. H. Dreisbach, “Handbook of Poisoning Prevention, Diagnosis and Treatment,” 12th Edition, Lange Medical, Los Altos, 1983.

[6]   N. Barlas, G. Selmanoglu, A. Kockaya and S. Songur “Effects of Carbendazim on Rat Thyroid, Parathyroid, Pituitary and Adrenal Glands and Their Hormones,” Human and Experimental Toxicology, Vol. 21, No. 4, 2002, pp. 217-221. doi:10.1191/0960327102ht187oa

[7]   G. Selmanoglu, N. Barlas, S. Songür and E. A. Kockaya, “Carbendazim-Induced Haematological, Biochemical and Histopathological Changes to the Liver and Kidney of Male Rats,” Human and Experimental Toxicology, Vol. 20, No. 12, 2001, pp. 625-630. doi:10.1191/096032701718890603

[8]   M. R. Gawande, J. A. Ganaie, A. Ramtake and V. K. Shrivastava, “Carbendazim Induced Histopathological Changes in Testis and Epididymis and Some Enzymes Activities in Testis of Rattus rattus,” Journal of Experimental Zoology (India), Vol. 12, No. 1, 2009, pp. 153156.

[9]   V. Muthuviveganandavel, P. Muthuraman, S. Muthu and K. J. Srikumar, “Toxic Effects of Carbendazim at Low Dose Levels in Male Rats,” Journal of Toxicological Sciences, Vol. 33, No. 1, 2008, pp. 33-30. doi:10.2131/jts.33.25

[10]   S. Logani, M. C. Chen, T. Tran, T. Le and R. B. Raffa, “Actions of Ginkgo Biloba Related to Potential Utility for the Treatment of Conditions Involving Cerebral Hypoxia,” Life Sciences, Vol. 67, No. 12, pp. 1389-1396. doi:10.1016/S0024-3205(00)00741-4

[11]   B. J. Diamond, S. S. C. hiflett, N. Feiwel, R. J. Matheis, O. Noskin, J. A. Richards and N. E. Schoenberger, “Ginkgo biloba Extract: Mechanisms and Clinical Indications,” Archives of Physical Medicine and Rehabilitation, Vol. 81, No. 5, 2000, pp. 668-678. doi:10.1053/mr.2000.3840

[12]   J. Shen, J. Wang, B. Zhao, J. Hou, T. Gao and W. Xin “Effects of EGb 761 on Nitric Oxide and Oxygen Free Radicals, Myocardial Damage and Arrhythmia in Ischemia-Reperfusion Injury in Vivo,” Biochimica et Biophysica Acta, Vol. 1406, No. 3, 1998, pp. 228-236. doi:10.1016/S0925-4439(98)00007-6

[13]   C. J. Chao and C. C. Chu, “Effect of Ginkgo biloba Extract on Cell Proliferation and Cytotoxicity in Human Hepatocellular Carcinoma Cells,” World Journal of Gastroenterology, Vol. 10, No. 1, 2004, pp. 37-41.

[14]   Y. L. Yang, Y. W. Su, M. C. Ng, C. L. Chang and K. T. Lu, “Extract of Ginkgo biloba EGb 761 Facilitates Fear Conditioning Measured by Fear-Potentiated Startle,” Neuroscience Letters, Vol. 383, No. 1-2, 2005, pp. 145-150. doi:10.1016/j.neulet.2005.04.003

[15]   R. Masteikova, J. Muselik, J. Bernatoniene and R. Bernatoniene, “Antioxidative Activity of Ginkgo, Echinacea and Ginseng Tinctures,” Medicina (Kaunas), Vol. 43, No. 4, 2007, pp. 306-309.

[16]   S. Mahadevan and Y. Park, “Multifaceted Therapeutic Benefits of Ginkgo biloba L.: Chemistry Efficacy, Safety and Uses,” Journal of Food Science, Vol. 73, No. 1, 2008, pp. 14-19. doi:10.1111/j.1750-3841.2007.00597.x

[17]   J. Qi, Y. Liu, Q. Li and X. Chen, “Effect of Ginkgo biloba Extract against Pulmonary Fibrosis and Its Mechanisms,” Zhongguo Zhong Yao Za Zhi, Vol. 35, No. 22, 2010, pp. 3043-3047.

[18]   M. M. Harputluoglu, U. Demirel and H. Ciralik, “Protective Effects of Gingko biloba on Thioacetamide-Induced Fulminant Hepatic Failure in Rats,” Human and Experimental Toxicology, Vol. 25, No. 12, 2006, pp. 705-713. doi:10.1177/0960327106073827

[19]   G. Sener, L. Kabasakal, B. M. Atasoy, C. Erzik, A. Velioglu-Ogünc, S. Cetinel, N. Gedik and B. C. Yegen “Ginkgo biloba Extract Protects against Ionizing Radiation-Induced Oxidative Organ Damage in Rats,” Pharmacological Research, Vol. 53, No. 3, 2006, pp. 241-252. doi:10.1016/j.phrs.2005.11.006

[20]   G. Sener, L. Kabasakal, M. Yüksel, N. Gedik and Y. Alican, “Hepatic Fibrosis in Biliary-Obstructed Rats Is Prevented by Ginkgo biloba Treatment,” World Journal of Gastroenterology, Vol. 11, No. 35, 2005, pp. 5444-5449.

[21]   M. A. Al-Attar, “Attenuating Effect of Ginkgo biloba Leaves Extract on Liver Fibrosis Induced by Thioacetamide in Mice,” Journal of Biomedicine and Biotechnology, Vol. 2012, 2012, Article ID: 761450. doi:10.1155/2012/761450

[22]   S. A. Sakr, H. A. Mahran and A. M. Abdel-Maksoud “Suppressive Effect of Ginkgo biloba Extract (EGb761) on Topsin Induced Ovarian Toxicity and Oxidative Stress in Albino Rats,” Journal of Applied Pharmaceutical Science, Vol. 1, No. 4, 2011, pp. 46-54.

[23]   S. A. Metwally, H. A. Abdel-latif, H. M. Fawzy and A. Hamdy, “The Protective Effect of Linseed Oil against Carbendazim Induced Testicular Toxicity in Rats,” European Journal of Scientific Research, Vol. 49, No. 2, 2011, pp. 208-224.

[24]   J. A. Kiernan, “Histological and Histochemical Methods, Theory and Practice,” Pergamon Press, New York, 1981

[25]   A. G. E. Pearse, “Histochemistry, Theoretical and Applied,” 23rd Edition, Churchill Livingstone, London, 1972.

[26]   S. Reitman and S. Frankel, “Colorimetric Determination of Glutamic Oxaloacetic and Glutamic Pyruvite Transaminase,” Journal of Clinical Pathology, 1975, pp. 28-56.

[27]   H. A. Lamfon “Protective Effect of Ginger (Zingiber officinale) against Metalaxyl Induced Hepatotoxicity in Albino Mice,” Journal of American Science, Vol. 7, No. 6, 201, pp. 1093-1100.

[28]   P. S. Waghe, P. S. Saini, S. Rampal, N. Prakash and L. V. Lokesh, “Sub-Chronic Exposure to Carbendazim Induces Biochemical and Hematological Alterations in Male Goats,” Toxicological & Environmental Chemistry, Vol. 95, No. 2, 2013, pp. 330-336. doi:10.1080/02772248.2013.770859

[29]   S. Sherlock, “Disease of the Liver and Biliary System,” 8th Edition, Blackwell Scientific Publication, Oxford, 1981.

[30]   M. P. Mahadevaswami, U. C. Jardaramkunti, M. B. Hiremath and B. B. Kaliwal, “Effect of Mancozeb on Ovarian Compensatory Hypertrophy and Biochemical Constituents in Hemicastrated Albino Rat,” Reproductive Toxicology, Vol. 14, No. 2, 2001, pp. 127-134.

[31]   A. R. Shakoori, S. S. Ali and M. A. Saleem, “Effect of Six Months Feeding of Cypermethrin on the Blood and Liver of Albino Rats,” Journal of Biochemical Toxicology, Vol. 3, 1988, pp. 59-72. doi:10.1002/jbt.2570030107

[32]   S. A. Sakr and S. Y. Shalaby, “Metiram-Induced Histological and Histochemical Alterations in Liver and Kidney of Pregnant Mice,” Life Science Journal, Vol. 9, No. 1, 2012, pp. 71-76.

[33]   S. A. Sakr, H. A. A. Samei and M. E. Soliman, “Exploring Hepatotoxicity of Benomyl: Histological and Histochemical Study on Albino Rats,” Journal of Medical Sciences, Vol. 4, No. 1, 2004, pp. 77-83.

[34]   A. A. Fouda, M. El-Shishtawy, A. H. Rizk, G. Abdel Salam, M. B. Bakr and A. El-Badway, “Ameliorative Effect of Ginkgo biloba Extract on Gamma Radiation Injury: Histological and Histochemical Evaluation in Rats,” Mansoura Journal of Forensic Medicine and Clinical Toxicology, Vol. 17, No. 2, 2009, pp. 43-62.

[35]   L. Ji, X. X. Yin, Z. M. Wu, J. Y. Wang, Q. Lu and Y. Y. Gao, “Ginkgo biloba Extract Prevents Glucose-Induced Accumulation of ECM in Rat Mesangial Cells,” Phytotherapy Research, Vol. 23, No. 4, 2009, pp. 477-485. doi:10.1002/ptr.2652

[36]   D. Tang, Z. Zhang, Y. Gao, Y. Wei and L. Han, “Protective Effects of Serum Containing Ginkgo biloba Extract on Glomerulosclerosis in Rat Mesangial Cells,” Journal of Ethnopharmacology, Vol. 124, No. 1, 2009, pp. 26-33. doi:10.1016/j.jep.2009.04.017

[37]   Y. Taki, Y. Yamazaki, F. Shimura, S. Yamada and K. Umegaki, “Time-Dependent Induction of Hepatic Cytochrome P450 Enzyme Activity and mRNA Expression by Bilobalide in Rats,” Journal of Pharmacological Sciences, Vol. 109, No. 3, 2009, pp. 459-462. doi:10.1254/jphs.08198SC

[38]   K. A. Shenoy, S. N. Somayaji and K. L. Bairy, “Hepatoprotective Effects of Ginkgo biloba against Carbon Tetrachloride Induced Hepatic Injury in Rats,” Indian Journal of Pharmacology, Vol. 33, No. 4, 2001, pp. 260-266.

[39]   S. Q. Liu, J. P. Yu, H. L. Chen, S. M. Luo, H. S. Chen and H. G. Yu, “Therapeutic Effects and Molecular Mechanisms of Ginkgo biloba Extract on Liver Fibrosis in Rats,” The American Journal of Chinese Medicine, Vol. 34, No. 1, 2006, pp. 99-114. doi:10.1142/S0192415X06003679

[40]   C. F. Zhang, C. Q. Zhang, Y. H. Zhu, J. Wang, H. W. Xu and W. H. Ren, “Ginkgo biloba Extract EGb 761 Alleviates Hepatic Fibrosis and Sinusoidal Microcirculation Disturbance in Patients with Chronic Hepatitis B,” Gastroenterology Research, Vol. 1, 2008, pp. 20-28.

[41]   S. I. Aleynik, M. A. Leo, X. Ma, M. K. Aleynik and C. S. Lieber, “Polyenylphosphatidylcholine Prevents Carbon Tetrachloride-Induced Lipid Peroxidation While It Attenuates Liver Fibrosis,” Journal of Hepatology, Vol. 27, No. 3, 1997, pp. 554-561. doi:10.1016/S0168-8278(97)80361-3

 
 
Top