Using a rat oxidative stress-induced femoral head
osteonecrosis model, we determined the presence/ absence and timing of the
generation of hypoxia in the femoral head. DL-Buthionine-(S,R)-sulfoximine (BSO)
500 mg/kg was administered intraperitoneally to male Wistar rats. The rats were
killed at 1, 3, 6, 12 hours, and 1, 3, 5 days after BSO administration, and the
bilateral femora were removed. A group not administered BSO (control group)
was also studied (each group n = 5). In the femoral heads of each group, the expression
of hypoxia-inducible factor-1 alpha (HIF-1α)
as an index of hypoxia was confirmed by the Western blot method, and quantified
using analytical software. In the femoral head increased HIF-1α expression was found in all groups
from 1 hour after BSO administration (p < 0.05). In particular, in all
specimens of the group 3 hours after BSO administration the most intense
expression of HIF-1α amounting to
about 13-fold of that of control group was noted (p < 0.001). The present results
suggested that in the extremely short period of 3 hours after BSO
administration hypoxia severe enough to cause osteonecrosis was induced by
oxidative stress in the rat femoral head.
Cite this paper
Ichiseki, T. , Kaneuji, A. , Kaneko, S. , Ueda, S. , Ueda, Y. , Yonekura, H. , Fukui, K. and Matsumoto, T. (2013) The timing and extent of intraosseous hypoxia in the oxidative stress-induced rat osteonecrosis model. Advances in Bioscience and Biotechnology, 4, 814-817. doi: 10.4236/abb.2013.48107.
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