ABSTRACT This study examined cells contained in needles used for the collection of breast fine needle aspirates for the detection of malignant cells trapped in the needles. Remnants of cells contained in 50 needles used for the collection of scanty breast fine needle aspirates were examined by the liquid based cytology technique and compared with the conventional cytological technique of specimens in the corresponding syringes. The breast specimens were collected with clean sterile needles attached to the syringes. Smears were made and stained by the conventional method. The needles were removed from the syringes and a fixative was withdrawn into the syringes and the syringes were recapped with the needles. The fixative containing the specimen was then completely discharged into a centrifuge tube through the needles and treated by the liquid based cytology technique. The study revealed that cells were found trapped in all the needles used for the collection of breast FNA. 6% of them were positive for malignancy, similar to results obtained in the conventional method. Needles used for the collection of breast FNA should be examined before malignancy is completely ruled out particularly in extremely scanty specimens with a clinical suspicion of malignancy.
Cite this paper
nullA. Godwin, I. Sina, B. Julius, O. Daniel and M. Ahmad, "Liquid Based Cytology of Cell Remnants in Needles Used for Breast Fine Needle Aspiration," Journal of Cancer Therapy, Vol. 1 No. 4, 2010, pp. 192-194. doi: 10.4236/jct.2010.14029.
 A. N. Kavatkar, C. A. Nagwanshi and S.M. Dabak, “Study of a Manual Method of Liquid-based Cervical Cytology.” Indian Journal of Pathology & Microbiology, Vol.51, No. 2, 2008, pp. 190-4.
ARW Williams, “Liquid-based Cytology and Conventional Smears Compared over Two 12-month Periods,” Cytopathology. Vol.17, No. 2, 2006, pp. 82-85.
B. Weynand, M. Berlière, E. Haumont, F. Massart, A. Pourvoyeur, P. Beri, et al. “A New Liquid-based Cytology Technique: Thermo Shandon's Papspin,” Acta Cytologica Vol.47, No. 2, 2003, pp. 149-53.
C. Bergeron and F. Fagnani, “Performance of a New Liquid-based Cervical Screening Technique in the Clinical Setting of a Large French laboratory.” Acta Cytologica Vol.47, No. 5, 2003, pp. 753-61.
D. Davey and R.J. Zarbo, “Introduction and Commentary, Strategic Science Symposium.” Archives of Pathology and Laboratory Medicine, Vol.12 No. 5, 2003, pp. 927-9.
C. Garbar, C. Mascaux and V. Fontaine, “Efficiency of an Inexpensive Liquid-based Cytology Performed by Cytocentrifugations: A Comparative Study Using the Histology as Reference Standard,” CytoJournal Vol. 2, No. 15, 2005, pp. 15.
M. Bibbo, W. J. Klump, J. DeCecco and A. J. Kovatich, “Procedure for Immunocytochemical Detection of P16INK4A Antigen in Thin-layer, Liquid-based Specimens,” Acta Cytologica, Vol.46, No.1, 2002, pp. 25-9.
L. Luzzatto, C. van Haaften and M. E. Boon, “Proliferation Patterns of Cervical Cells as Visualized in Leiden liquid Cytology Slides.” Diagnostic Cytopathology, Vol. 31, No.1, 2004, pp. 5-9.
R. S. Hoda, “Non-gynecologic Cytology on Liquid Based Preparations: A Morphologic Review of Facts and Artifacts.” Diagnostic Cytopathology, Vol. 35, No.10, 2007, pp. 621-34.
H. Sartelet, E. Lagonette, M. Lorenzato, Duval I., Lechki C., Rigaud C. et al., “Comparison of Liquid Based Cytology and Histology for the Evaluation of HER-2 Status Using Immunostaining and CISH in Breast Carcinoma.” Journal of Clinical Pathology, Vol. 58, No.8, 2005, pp. 864-871.
Veneti S, Daskalopoulou D, Zervoudis S, Papasotiriou E and Ioannidou-Mouzaka L., “Liquid-based Cytology in Breast Fine Needle Aspiration. Comparison with the Conventional Smear.” Acta Cytologica, Vol. 47, No.2, 2003, pp. 188-92.
Komatsu K., Nakanishi Y., Seki T., Yoshino A., Fuchinoue F., Amano S., Komatsu A., Sugitani M. and Nemoto N., “Application of Liquid-based Preparation to Fine Needle Aspiration Cytology in Breast Cancer.” Acta Cytologica, Vol. 52, No.5, 2008, pp. 591-6.
Avwioro O. G., “Textbook of Histochemistry and Tissue Pathology,” 2nd Edition, Claverianun Press, Ibadan, Nigeria.