ABSTRACT The seed size, seed mass, and growth rate of transgenic Arabidopsis plants containing PgTIP1, a ginseng tonoplast aquaporin gene, are significantly higher than those of wild-type Arabidopsis plants. Whole genome expression and bioinformatics analysis, including analysis of co-expression networks and transcription factors (Tfscan), were used to determine the key genes that are activated after the expression of PgTIP1 and the transcription factors that play important roles in the regulation of the genes controlling growth of Arabidopsis thaliana seeds by using transgenic Arabidopsis plants containing PgTIP1. Differential gene analysis showed that transformation of exogenous PgTIP1 to Arabidopsis induced endogenous gene expression changes. Analysis of gene co-expression networks revealed 2 genes, PIP1 (plasma membrane aquaporin 1 gene) and RD26 (responsive to desiccation 26 gene; a NAC transcription factor), that were localized in the core of the networks. Analysis of the transcriptional regulation network of transgenic Arabidopsis plants containing PgTIP1 showed that PIP1 and RD26 were regulated via DNA binding with a finger domain on transcription factor 2 (Dof2). In this study, we demonstrated that Dof2 induces up-regulation of PIP1 and RD26 after transformation with PgTIP1. The results of this study provide a new means for conducting research into and controlling growth of Arabidopsis thaliana seeds.
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nullChen, H. , Ying, L. , Jin, J. , Li, Q. and Cai, W. (2010) Determining the transcriptional regulation pattern of PgTIP1 in transgenic Arabidopsis thaliana by constructing gene. Advances in Bioscience and Biotechnology, 1, 384-390. doi: 10.4236/abb.2010.15051.
 Lin, W.L., Peng, Y.H., Li, G.W., Arora, R., Tang, Z.C., Su, W.A. and Cai, W.M. (2007) Isolation and functional characterization of PgTIP1, a hormone-autotrophic cells-specific tonoplast aquaporin in ginseng. Journal of Experimental Botany, 58(5), 947-956.
Mao, J., Zhang, Y.C., Sang, Y., Li, Q.H. and Yang, H.Q. (2005) A role for Arabidopsis cryptochromes and COP1 in the regulation of stomatal opening. Proceedings of the National Academy of Sciences, USA, 102(34), 12270-12275.
Clough, S.J. and Bent, A.F. (1998) Floral dip: A simplified method for Agrobacterim-mediated transformation of Arabidopsis thaliana. Plant Journal, 16(6), 725-742.
Weigel, D. and Glazebrook, J. (2002) Arabidopsis: a laboratory manual. Cold Spring Harbor Laboratory Press, New York.
Wright, G.W. and Simon, R.M. (2003) A random variance model for detection of differential gene expression in small microarray experiments. Bioinformatics, 19(15), 2448-2455.
Yang, H., Crawford, N., Lukes, L., Finney, R., Lancaster, M. and Hunter, K.W. (2005) Metastasis predictive signature profiles pre-exist in normal tissues. Clinical and Experimental Metastasis, 22(7), 593-603.
Clarke, R., Ressom, H.W., Wang, A., Xuan , J., Liu, M.C., Gehan, E.A. and Wang, Y. (2008) The properties of high-dimensional data spaces: Implications for exploring gene and protein expression data. Nature Reviews Cancer, 8(1), 37-49.
Pujana, M.A., Han, J.D., Starita, L.M., Stevens, K.N., Tewari, M., Ahn, J.S., Rennert, G., Moreno, V., Kirchhoff, T., Gold, B., Assmann, V., Elshamy, W.M., Rual, J.F., Levine, D., Rozek, L.S., Gelman, R.S., Gunsalus, K.C., Greenberg, R.A., Sobhian, B., Bertin, N., Venkatesan, K., Ayivi-Guedehoussou, N., Sole, X., Hernandez, P., Lazaro, C., Nathanson, K.L., Weber, B.L., Cusick, M.E., Hill, D.E., Offit, K., Livingston, D.M., Gruber, S.B., Parvin, J.D. and Vidal, M. (2007) Network modeling links breast cance
Prieto, C., Risueno, A., Fontanillo, C. and De las Rivas, J. (2008) Human gene coexpression landscape: Confident network derived from tissue transcriptomic profiles. PLoS One, 3(12), e3911.
Carlson, M.R., Zhang, B., Fang, Z., Mischel, P.S., Horvath, S. and Nelson, S.F. (2006) Gene connectivity, function, and sequence conservation: Predictions from modular yeast coexpression networks. BMC Genomics, 7, 40.
Barabasi, A.L. and Oltvai, Z.N. (2004) Network biology: understanding the cell’s functional organization. Nature Reviews Genetics, 5(2), 101-113.
Ravasz, E., Somera, A.L., Mongru, D.A., Oltvai, Z.N. and Barabási, A.L. (2002) Hierarchical organization of modularity in metabolic networks. Science, 297(5586), 1551-1555.
Vermeirssen, V., Barrasa, M.I., Hidalgo, C.A., Babon, J.A., Sequerra, R., Doucette-Stamm, L., Barabási, A.L. and Walhout, A.J. (2007) Transcription factor modularity in a gene-centered C. elegans core neuronal protein-DNA interaction network. Genome Research, 17(7), 1061-1071.
Franka, S., Melvin, T. T., Claudio, L., Andrea, S. and Ralf, K. (2002) PIP1 plasma membrane aquaporins in tobacco: From cellular effects to function in plants. Plant Cell, 14(4), 869-876.
Olivier, P., Colette, T., Alexandre, G., Yann, B., Rapha?l, M., Anton, R.S. and Christophe, M. (2010) A PIP1 Aquaporin contributes to hydrostatic pressure-induced water transport in both the root and rosette of arabidopsis. Plant Physiology, 152(3), 1418-1430.
Fujita, M., Fujita, Y., Maruyama, K., Seki, M., Hiratsu, K., Ohme-Takagi, M., Tran, L.S., Yamaguchi-Shinozaki, K. and Shinozaki, K. (2004) A dehydration-induced NAC protein, RD26, is involved in a novel ABA-dependent stress-signaling pathway. Plant Journal, 39(6), 863-876.
Shuichi, Y. (2004) Dof domain proteins: Plant-specific transcription factors associated with diverse phenomena unique to plants. Plant and Cell Physiology, 45(4), 386-391.