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 FNS  Vol.4 No.8 A , August 2013
Major Phenolics in Yerba Mate Extracts (Ilex paraguariensis) and Their Contribution to the Total Antioxidant Capacity
Lorena Deladino, Aline Schneider Teixeira, Mario Reta, Antonio D. Molina García, Alba S. Navarro, Miriam N. Martino
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Abstract: Yerba mate (Ilex paraguariensis) is an indigenous crop which is highly consumed as an infusion in the South American subtropical forest. It has a high concentration of antioxidant substances, providing health benefits and helping to prevent diseases. The objectives of this work were to characterize an aqueous yerba mate extract by spectrophotometric and chromatographic (HPLC) methods and to study the effect of the freeze-drying process on the polyphenols profile and antioxidant activity, determined by a novel method. An aqueous extract was obtained and lyophilized to obtain a yerba mate powder with antioxidant properties. The extracts showed a high polyphenol content, determined by Folin-Ciocalteau and HPLC, and a high antioxidant activity towards the DPPH· radical and after the recently developed method of photochemiluminescence. A linear correlation was found between Folin-Ciocalteau and DPPH methods for lyophilized samples. HPLC analysis allowed determining antioxidant components like rutin, caffeine and chlorogenic acid. Lyophilization caused a decrease in total polyphenol content and antiradical activity of the extracts and this fact was mainly attributed to changes in the chlorogenic acid related compounds and rutin structures, after their photochemiluminescence data. The photoluminiscent method proves to be an advantageous approach for antioxidant capacity determination.
Keywords: Yerba Mate; Natural Antioxidants; Freeze Drying; DPPH; HPLC; PCL
Cite this paper: L. Deladino, A. Teixeira, M. Reta, A. García, A. Navarro and M. Martino, "Major Phenolics in Yerba Mate Extracts (Ilex paraguariensis) and Their Contribution to the Total Antioxidant Capacity," Food and Nutrition Sciences, Vol. 4 No. 8, 2013, pp. 154-162. doi: 10.4236/fns.2013.48A019.
References

[1]   N. Bracesco, et al., “Antioxidant Activity of a Botanical Extract Preparation of Ilex Paraguariensis: Prevention of DNA Double-Strand Breaks in Saccharomyces Cerevisiae and Human Low-Density Lipoprotein Oxidation,” The Journal of Alternative and Complementary Medicine, Vol. 9, No. 3, 2003, pp. 379-387. doi:10.1089/107555303765551606

[2]   N. Bracesco, A. G. Sanchez, V. Contreras, T. Menini and A. Gugliucci, “Recent Advances on Ilex Paraguariensis Research: Minireview,” Journal of Ethnopharmacology, Vol. 136, No. 3, 2011, pp. 378-384. doi:10.1016/j.jep.2010.06.032

[3]   A. Gugliucci and A. J. Stahl, “Low Density Lipoprotein Oxidation Is Inhibited by Extracts of Ilex Paraguariensis,” Biochemistry & Molecular Biology International, Vol. 35, No. 1, 1995, pp. 47-56.

[4]   N. Lunceford and A. Gugliucci, “Ilex Paraguariensis Extracts Inhibit AGE Formation More Efficiently than Green Tea,” Fitoterapia, Vol. 76, No. 5, 2005, pp. 419-427.

[5]   C. Anesini, G. Ferraro and R. Filip, “Peroxidase-Like Activity of Ilex Paraguariensis,” Food Chemistry, Vol. 97, No. 3, 2006, pp. 459-464.

[6]   D. Rodriguez de Sotillo, M. Hadley and E.T. Holm, “Potato Peel Waste: Stability and Antioxidant Activity of a Freeze-Dried Extract,” Journal of Food Science, Vol. 59, No. 5, 1994, pp. 1031-1033. doi:10.1111/j.1365-2621.1994.tb08182.x

[7]   R. Filip, S. B. Lotito, G. Ferraro and C. G. Fraga, “Antioxidant Activity of Ilex Paraguariensis and Related Species,” Nutrition Research, Vol. 20, No. 10, 2000, pp. 1437-1446. doi:10.1016/S0271-5317(00)80024-X

[8]   C. I. Heck, M. Schmalko and E. González de Mejía, “Effect of Growing and Drying Conditions on the Phenolic Composition of Mate Teas (Ilex Paraguariensis),” Journal of Agricultural and Food Chemistry, Vol. 56, No. 18, 2008, pp. 8394-8403. doi:10.1021/jf801748s

[9]   G. Popov and I. Lewin, “Photochemiluminescent Detection of Antiradical Activity; IV: Testing of Lipid-Soluble Antioxidants,” Journal of Biochemical and Biophysical Methods, Vol. 31, No. 1-2, 1996, pp. 1-8.

[10]   W. Brand-Williams, M. E. Cuvelier and C. Berset, “Use of a Free Radical Method to Evaluate Antioxidant Activity,” LWT—Food Science and Technology, Vol. 28, No. 1, 1995, pp. 25-30.

[11]   C. Sánchez-Moreno, J. A. Larrauri and F. Saura-Calixto, “A Procedure to Measure the Antiradical Efficiency of Polyphenols,” Journal of the Science of Food and Agriculture, Vol. 76, No. 2, 1998, pp. 270-276. doi:10.1002/(SICI)1097-0010(199802)76:2<270::AID-JSFA945>3.0.CO;2-9

[12]   P. Dugo, et al., “High Efficiency Liquid Chromatography Techniques Coupled to Mass Spectrometry for the Characterization of Mate Extracts,” Journal of Chromatography A, Vol. 1216, No. 43, 2009, pp. 7213-7221. doi:10.1016/j.chroma.2009.08.030

[13]   S. Isolabella, et al., “Study of the Bioactive Compounds Variation during Yerba Mate (Ilex Paraguariensis) Processing,” Food Chemistry, Vol. 122, No. 3, 2010, pp. 695699. doi:10.1016/j.foodchem.2010.03.039

[14]   V. R. Sinija, H. N. Mishra and S. Bal, “Process Technology for Production of Soluble Tea Powder,” Journal of Food Engineering, Vol. 82, No. 3, 2007, pp. 276-283. doi:10.1016/j.jfoodeng.2007.01.024

[15]   L. Liu, et al., “Determination of Polyphenolic Content and Antioxidant Activity of Kudingcha Made from Ilex Kudingcha C.J. Tseng,” Food Chemistry, Vol. 112, No. 1, 2009, pp. 35-41. doi:10.1016/j.foodchem.2008.05.038

[16]   R. Jaiswal, T. Sovdat, F. Vivan and N. Kuhnert, “Profiling and Characterization by LC-MSn of the Chlorogenic Acids and Hydroxycinnamoylshikimate Esters in Mate (Ilex paraguariensis),” Journal of Agricultural and Food Chemistry, Vol. 58, No. 9, 2010, pp. 5471-5484.

[17]   R. Jaiswal, J. Kiprotich and N. Kuhnert, “Determination of the Hydroxycinnamate Profile of 12 Members of the Asteraceae Family,” Phytochemistry, Vol. 72, No. 8, 2011, pp. 781-790. doi:10.1016/j.phytochem.2011.02.027

[18]   R. Filip, P. López, G. Giberti, J. Coussio and G. Ferraro, “Phenolic Compounds in Seven South American Ilex Species,” Fitoterapia, Vol. 72, No. 7, 2001, pp. 774-778. doi:10.1016/S0367-326X(01)00331-8

[19]   C. Anesini, S. Turner, L. Cogoi and R. Filip, “Study of the Participation of Caffeine and Polyphenols on the Overall Antioxidant Activity of Mate (Ilex Paraguariensis),” LWT —Food Science and Technology, Vol. 45, No. 2, 2012, pp. 299-304.

[20]   S. Chandra and E. G. D. Mejía, “Polyphenolic Compounds, Antioxidant Capacity, and Quinone Reductase Activity of an Aqueous Extract of Ardisia Compressa in Comparison to Mate (Ilex Paraguariensis) and Green (Camellia Sinensis) Teas,” Journal of the Science of Food and Agriculture, Vol. 52, No. 11, 2004, pp. 3583-3589.

[21]   A. K. Atoui, A. Mansouri, G. Boskou and P. Kefalas, “Tea and Herbal Infusions: Their Antioxidant Activity and Phenolic Profile,” Food Chemistry, Vol. 89, No. 1, 2005, pp. 27-36.

[22]   V. L. Singleton, R. Orthofer and R. M. Lamuela-Raventós, “Methods in Enzymology,” Academic Press, 1999, pp. 152-178.

[23]   D. H. M. Bastos, E. Y. Ishimoto, M. Ortiz, M. Marques, A. Fernando Ferri and E. A. F. S. Torres, “Essential Oil and Antioxidant Activity of Green Mate and Mate Tea (Ilex Paraguariensis) Infusions,” Journal of Food Composition and Analysis, Vol. 19, No. 6-7, 2006, pp. 538-543. doi:10.1016/j.jfca.2005.03.002

[24]   V. C. Dall’Orto, “Comparison of Tirosinase Biosensor and Colorimetric Method for Polyphenol Analysis in Different Kinds of Teas,” Analytical Letters, Vol. 38, No. 1, 2005, pp. 19-33.

[25]   L. Bravo, L. Goya and E. Lecumberri, “LC/MS Characterization of Phenolic Constituents of Mate (Ilex Paraguariensis, St. Hil.) and Its Antioxidant Activity Compared to Commonly Consumed Beverages,” Food Research International (Ottawa, Ont.), Vol. 40, No. 3, 2007, pp. 393405.

[26]   H. Zhao, et al., “Evaluation of Antioxidant Activities and Total Phenolic Contents of Typical Malting Barley Varieties,” Food Chemistry, Vol. 107, No. 1, 2008, pp. 296304. doi:10.1016/j.foodchem.2007.08.018

[27]   L. Deladino, P. S. Anbinder, A. S. Navarro and M. N. Martino, “Encapsulation of Natural Antioxidants Extracted from Ilex Paraguariensis,” Carbohydrate Polymers, Vol. 71, No. 1, 2008, pp. 126-134. doi:10.1016/j.carbpol.2007.05.030

[28]   I. Parejo, C. Codina, C. Petrakis and P. Kefalas, “Evaluation of Scavenging Activity Assessed by Co(II)/EDTAInduced Luminal Chemiluminescence and DPPH Free Radical Assay,” Journal of Pharmacological and Toxicological Methodological, Vol. 44, No. 3, 2000, pp. 507512.

[29]   I. Parejo, et al., “Comparison between the Radical Scavenging Activity and Antioxidant Activity of Six Distilled and Nondistilled Mediterranean Herbs and Aromatic Plants,” Journal of Agricultural and Food Chemistry, Vol. 50, No. 23, 2002, pp. 6882-6890.

 
 
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