FNS  Vol.4 No.8 A , August 2013
Nutritional, Antioxidant and Anti-Inflammatory Properties of Cyclanthera pedata, an Andinean Fruit and Products Derived from Them
Abstract: There is an increasing interest in food plant with health-giving effects. The aim of this research was to evaluate the phytochemical and nutraceutical profiles of Cyclanthera pedata fruits, a native fruit of the Andean region used by Incas communities called achojcha. Soluble protein (SP), total sugar (TS), free phenolic compounds (FPC), ascorbic acid (AA), total monomeric anthocyanin (TMA), condensed and hydrolizable tannin (CT, HT) were evaluated by sprectrophotometric methods in fresh fruits with and without heating and in flour obtained from fruits. Multielemental composition by ICP-MS was done. Antioxidant activity (AOA) was determined by ABTS and Linoleic acid-b-carotene methods. The inhibitory capacity of LOX, a pro-inflammatory enzyme was also analyzed. The achojcha fresh fruits and achojcha flour showed low amount calories. The flour was high in potassium (7400 mg·K/100 g) and low in sodium (77 mg Na/100 g). High levels of FPC (670 mg GAE/100 g) were found together with elevated levels of AA (123 mg AA/ 100 g). The TMA (0.6 mg C3-GE/100 g) as well as HT (3.4 mg PB2E/100 g) were also detected in flour. All preparations obtained with fresh and dried fruits showed AOA with SC50 values between 1.8 to 14.5 μg GAE/mL. Inhibitory capacity on LOX was also demonstrated (IC50 values of 40 μg GAE/mL). The fresh fruits and flour showed nutraceutical characteristics that are demanded by functional food and could be used as dietary supplement.
Cite this paper: M. Rivas, D. Vignale, R. Ordoñez, I. Zampini, M. Alberto, J. Sayago and M. Isla, "Nutritional, Antioxidant and Anti-Inflammatory Properties of Cyclanthera pedata, an Andinean Fruit and Products Derived from Them," Food and Nutrition Sciences, Vol. 4 No. 8, 2013, pp. 55-61. doi: 10.4236/fns.2013.48A007.

[1]   Y. Wang, Y. Wang, B. McNeil and L. M. Harvey, “Maca: An Andean Crop with Multi-Pharmacological Functions,” Food Research International, Vol. 40, No. 7, 2007, pp. 783-792. doi:10.1016/j.foodres.2007.02.005

[2]   J. Lachman, E. C. Fernández and M. Orsák, “Yacon [Smallanthus sonchifolia (Poepp. et Endl.) H. Robinson] Chemical Composition and Use—A Review,” Plant Soil Environmental, Vol. 49, No. 6, 2003, pp. 283-290.

[3]   H. Dietschy, “L’antica Medicina Peruviana,” Ciba. Milano, No. 40, 1953, pp. 1318-1345.

[4]   H. Popenoe and D. C. Washington, “Lost Crops of the Incas: Little-Known Plants of the Andes with Promise for Worldwide Cultivation,” Vol. 428, 1990, pp. 206-209.

[5]   J. F. Macbride, “Cucurbitaceae Flora of Peru,” In: Field Museum of Natural History, Botanical Series, 1937, pp. 321-383.

[6]   P. Montoro, V. Carbone, F. de Simone, C. Pizza and N. de Tommasi, “Studies on the Constituents of Cyclanthera pedata Fruits: Isolation and Structure Elucidation of New Flavonoid Glycosides and Their Antioxidant Activity,” Journal of Agriculture and Food Chemistry, Vol. 49, No. 11, 2002, pp. 5156-5161. doi:10.1021/jf010318q

[7]   M. Monigatti, R Bussmann and C. Weckerle, “Medicinal Plant Use in Two Andean Communities Located at Different Altitudes in the Bolivar Province, Peru,” Journal of Ethnopharmacology, Vol. 145, No. 2, 2012, pp. 450-464. doi:10.1016/j.jep.2012.10.066

[8]   L. Dinan, J. Harmatha and R. Lafont, “Chromatographic Procedures for the Isolation of Plant Steroids,” Journal of Chromatographic A, Vol. 935, No. 1-2, 2001, pp. 105123. doi:10.1016/S0021-9673(01)00992-X

[9]   N. De Tommasi, F. De Simone, G. Speranza and C. Pizza, “Studies on the Constituents of Cyclanthera pedata (Caigua) Seeds: Isolation and Characterization of Six New Cucurbitacin Glycosides,” Journal of Agriculture and Food Chemistry, Vol. 44, No. 8, 1996, pp. 2020-2025. doi:10.1021/jf950532c

[10]   N. De Tommasi, F. De Simone, G. Speranza and C. Pizza, “Studies on the Constituents of Cyclanthera pedata Fruits: Isolation and Structure Elucidation of New Triterpenoid Saponins,” Journal of Agriculture and Food Chemistry, Vol. 47, No. 11, 1999, pp. 4512-4519. doi:10.1021/jf9900128

[11]   V. Carbone, P. Montoro, N. De Tommasi and C. Pizza, “Analysis of Flavonoids from Cyclanthera pedata Fruits by Liquid Chromatography/Electrospray Mass Spectrometry,” Journal of Pharmaceutics and Biomedical Analysis, Vol. 34, No. 2, 2004, pp. 295-304.

[12]   L. Galvez Ranilla, Y. Kwon, E. Apostolidis and K. Shetty, “Phenolic Compounds, Antioxidant Activity and in Vitro Inhibitory Potential against Key Enzymes Relevant for Hyperglycemia and Hypertension of Commonly Used Medicinal Plants, Herbs and Spices in Latin America,” Bioresource Technology, Vol. 101, No. 12, 2010, pp. 4676-4689. doi:10.1016/j.biortech.2010.01.093

[13]   F. E. Prado, J. A. Gonzalez, C. Boero and A. R. Sampietro, “A Simple and Sensitive Method for Determining Reducing Sugars in Plant Tissues. Application to Quantify the Sugar Content in Quinoa (Chenopodium quinoa Willd.) Seedlings,” Phytochemical Analysis, Vol. 9, No. 2, 1998, pp. 58-62.

[14]   M. Dubois, K. A. Gilles, J. K. Hamilton, P. A., Rebers and F. Smith, “Colorimetric Method for Determination of Sugars and Related Substances,” Analytical Chemistry, Vol. 28, No. 3, 1956, pp. 350-356. doi:10.1021/ac60111a017

[15]   M. Somogyi, “A New Reagent for the Determination of Sugar,” Journal of Biological Chemistry, Vol. 160, No. 1, 1945, pp. 61-68.

[16]   N. Nelson, “A Photometric Adaptation of the Somogyi Method for the Determination of Glucose,” Journal of Biological Chemistry, Vol. 153, 1944, pp. 375-380.

[17]   M. M. Bradford, “Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding,” Analytical Biochemistry, Vol. 72, No. 1-2, 1976, pp. 248-254. doi:10.1016/0003-2697(76)90527-3

[18]   AOAC, “Official Methods of Analysis,” 16th Edition, Association of Official Analytical Chemists, Arlington, 1998.

[19]   V. L. Singleton, R. Orthofer and R. M. Lamuela-Raventos, “Analysis of Total Phenols and Other Oxidation Substrates and Antioxidants by Means of Folin-Ciocalteu Reagent,” Method in Enzymology, Vol. 299, 1999, pp. 152178. doi:10.1016/S0076-6879(99)99017-1

[20]   B. W. Zoecklein, K. C. Fuelsang B. H. Gump and F. S. Nury, “Phenolic Compounds and Wine Color,” In: Van Nostrand Reinhold, Ed., Production Wine Analysis, New York, 1990, pp. 129-168. doi:10.1007/978-1-4615-8146-8_7

[21]   J. L. C. Lamaison and A. Carnet, “Teneurs en Principaux Flavonoides des Fleurs de Crataegus monogyna Jacq et de Crataegus laevigata (Poiret D. C) en Fonction de la Vegetation,” Pharmaceutica Acta Helvetia, Vol. 65, 1990, pp. 315-320.

[22]   M. Popova, S. Silici, O. Kaftanoglu and V. Bankova, “Antibacterial Activity of Turkish Propolis and Its Qualitative and Quantitative Chemical Composition,” Phytomedicine, Vol. 12, No. 3, 2005, pp. 221-228.

[23]   R. L. Prior, E. Fan, H. Ji, A. Howell, C. Nico, M. J. Payne and J. Reed, “Multilaboratory Validation of a Standar Method for Quantifying Proanthocyanidins in Cranberry Powders,” Journal of the Science of Food and Agriculture, Vol. 90, No. 9, 2010, pp. 1473-1478. doi:10.1002/jsfa.3966

[24]   K. H. Inoue and A. E. Hagerman, “Determination of Gallotannins with Rhodanine,” Analytical Biochemistry, Vol. 169, 1988, pp. 363-369.

[25]   J. Lee, R. W. Durst and R. E. Wrolstad, “Determination of Total Monomeric Anthocyanin Pigment Content of Fruits Juices, Beverages, Natural Colorants, and Wines by the pH Differential Method: Collaborative Study,” Journal of AOAC International, Vol. 88, No. 5, 2005, pp. 1269-1278.

[26]   D. B. Rodríguez-Amaya, “A Guide to Carotenoid Analysis in Foods,” ILDI Press, Washington DC, 1999.

[27]   L. Barros, S. Heleno, A. Carvalho and I. Ferreira, “Lamiaceae Often Used in Portuguese Folk Medicine as a Source of Powerful Antioxidants: Vitamins and Phenolics,” Food Science and Technology, Vol. 43, No. 3, 2010, pp. 544550. doi:10.1016/j.lwt.2009.09.024

[28]   R. Re, N. Pellegrini, A. Proteggente, A. Pannala, M. Yang and C. Rice-Evans, “Antioxidant Activity Applying an Improved ABTS Radical Cation Decolorization Assay,” Free Radical Biology and Medicine, Vol. 26, No. 9-10, 1999, pp. 1231-1237. doi:10.1016/S0891-5849(98)00315-3

[29]   A. A. Ordonez, D. Gomez, M. A. Vattuone and M. I. Isla, “Antioxidant Activity of Sechium edule (Jacq) Swartz,” Food Chemistry, Vol. 97, No. 3, 2006, pp. 452-458. doi:10.1016/j.foodchem.2005.05.024

[30]   I. B. Taraporewala and J. M. Kauffman, “Synthesis and Structure-Activity Relationship of Anti-Inflammatory 9, 10-Dihydro-9-oxo-2-acridine-alkanoic Acids and 4-(2-Carboxyphenyl) Aminobenzenealkanoic Acids,” Journal of Pharmaceutical Sciences, Vol. 79, No. 2, 1990, pp. 173178. doi:10.1002/jps.2600790219

[31]   C. Vasco, J. Ruales and A. Kamal-Eldin, “Total Phenolic Compounds and Antioxidant Capacities of Major Fruits from Ecuador,” Food Chemistry, Vol. 111, No. 4, 2008, pp. 816-823. doi:10.1016/j.foodchem.2008.04.054

[32]   A. Valente, T. Albuquerque, A. Sanchez-Silva and H. Costa, “Ascorbic Acid Content in Exotic Fruits: A Contribution to Produce Quality Data for Food Composition Databases,” Food Research International, Vol. 44, No. 7, 2011, pp. 2237-2242. doi:10.1016/j.foodres.2011.02.012

[33]   Expert Consultation Bangkak, “Human Vitamin and Mineral Requirements,” Report 07 a Joint FAO/OMS, 2001.

[34]   D. Konopacka, A. Seroczyńska, A. Korzeniewska, K. Jesionkowska, K. Niemirowicz-Szczytt and W. Plocharski, “Studies on the Usefulness of Cucurbita Maxima for the Production of Ready-to-Eat Dried Vegetable Snacks with a High Carotenoid Content,” LWT—Food Science and Technology, Vol. 43, No. 2, 2010, pp. 302-309. doi:10.1016/j.lwt.2009.08.012

[35]   A. Waldiceu, Verri Jr., T. M. C. Vicentini, M. Baracat, SR. Georgetti, D. R. Cardoso, T. M. Cunha, H. Ferreira, F. Q. Cunha, M. J. V. Fonseca and R. Casagrande, “Flavonoids as Anti-Inflammatory and Analgesic Drugs: Mechanisms of Action and Perspectives in the Development of Pharmaceutical Forms,” Bioactive Natural Products, Vol. 36, 2012, pp. 297-330.