objective of this study is to verify potential of various types of microorganisms during spray
drying and non-refrigerated storage that can be enhanced substantially by selecting suitable protective colloids.
Four selected probiotics tested are Lactbacillus plantarum B13 and B18, which
are the bacteria probiotics and Kluyveromyces lactis and Saccharomyces
blouradii, non-bacteria probiotics. Two levels of experiment occur starting
with formulation study of encapsulation agent followed by the viability study
of different probiotics after spray dry and two weeks nonrefrigerated storage.
The formulation of 30% of gum Arabic, 15% of gelatin and 45% of coconut oil can
homogenize well at least for two hours and can produce acceptable dried product
(below 4% of moisture content) at low outlet temperature (70℃ -75℃).
K. lactis, S. blouradii gives 2.57% and 2.4% of viability percentage after
spray drying process and 25.84% and 2.04% after two weeks nonrefrigerated
storage respectively. The colonies of non-probiotics bacteria after both
conditions are between 1010 and 106 cfu/mL which is among the accepted level
for industrial application. However, the survival of probiotics in a
spray-dried form during non-refrigerated storage is higher at low of moisture
content compared to others.
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