AJPS  Vol.4 No.7 B , July 2013
Field Application of Non-Pathogenic Verticillium dahliae Genotypes for Regulation of Wilt in Strawberry Plants
Abstract: Verticillium dahliae induced wilt disease in strawberry can cause severe yield losses and thus lead to inevitable negative economic impacts. Inoculation of plants with non-pathogenic strains of Verticillium was conducted as a biologic control agent (BCA) according to the concept that preoccupation of the ecologic niche rendered strawberry plants immune to infection with soil-borne pathogenic Verticillium. This concept was tested for economic viability in a field trial under commercial conditions. Results were reported for 2 years of field trials under practice conditions in two locations in Brandenburg, Germany. Inoculation was shown to have a positive effect of 20% of plants, while 30% of plants remain unaffected and of equally high vitality. However, 50%-0% of plants were impacted negatively, showing severe wilt symptoms up to total loss. The characteristic progression of wilt symptoms suggested an infestation caused by Phytophtora sp. and other pathogens. Further results showed that the main factor of the side effects was caused by different qualities of plant material in interaction to the inoculation with the BCA and only to a minor extent depended on pre-infestation of soils. We conclude that specific conditions, such as certified plant material or soil analysis for other pathogens besides Verticillium, avoided these side-effects relevant for commercial farming.
Cite this paper: K. Diehl, P. Rebensburg and P. Lentzsch, "Field Application of Non-Pathogenic Verticillium dahliae Genotypes for Regulation of Wilt in Strawberry Plants," American Journal of Plant Sciences, Vol. 4 No. 7, 2013, pp. 24-32. doi: 10.4236/ajps.2013.47A2004.

[1]   M. Heupel, “Verticillium Diseases in Germany: History, Significance and Managment,” Proceedings of the 11th International Verticillium Symposium, Gottingen, 5-8 May 2013, pp. 17-18.

[2]   D. C. Harris and J. R. Yang, “The Relationship between the Amount of Verticillium dahliae in Soil and the Incidence of Strawberry Wilt as a Basis for Disease Risk Prediction,” Plant Pathology, Vol. 45, No. 1, 1996, pp. 106-114. doi:10.1046/j.1365-3059.1996.d01-96.x

[3]   E. F. Fradin and B. P. H. J. Thomma, “Physiology and Molecular Aspects of Verticillium Wilt Diseases Caused by V. dahlia and V. alboatrum,” Molecular Plant Pathology, Vol. 7, No. 2, 2006, pp. 71-86. doi:10.1111/j.1364-3703.2006.00323.x

[4]   G. M. Malcolm, G. A. Kuldau, B. K. Gugino and M. del M. Jiménez-Gasco, “Hidden Host Plant Associations of Soilborne Fungal Pathogens: An Ecological Perspective,” Phytopathology, Vol. 103, No. 6, 2013, pp. 538-544. doi:10.1094/PHYTO-08-12-0192-LE

[5]   S. J. Klostermann, Z. K. Atallah, G. E. Vallad and K. V. Subbarao, “Diversity, Pathogenicity, and Management of Verticillium Species,” Annual Review of Phytopathology, Vol. 47, 2009, pp. 39-62. doi:10.1146/annurev-phyto-080508-081748

[6]   F. N. Martin, “Development of Alternative Strategies for Management of Soilborne Pathogens Currently Controlled with Methyl Bromide,” Annual Review of Phytopathogy, Vol. 41, 2003, pp. 325-350. doi:10.1146/annurev.phyto.41.052002.095514

[7]   F. J. López-Escudero and J. Mercado-Blanco, “Verticillium Wilt of Olive: A Case Study to Implement an Integrated Strategy to Control a Soil-Borne Pathogen,” Plant Soil, Vol. 344, No. 1-2, 2011, pp. 1-50. doi:10.1007/s11104-010-0629-2

[8]   D. V. Shaw, T. R. Gordon, J. Hansen and S. C. Kirkpatrick, “Relationship between the Extent of Colonization by Verticillium dahliae and Symptom Expression in Strawberry (Fragaria × ananassa) Genotypes Resistant to Verticillium Wilt,” Plant Pathology, Vol. 59, No. 2, 2010, pp. 376-381. doi:10.1111/j.1365-3059.2009.02203.x

[9]   D. C. Harris, J. R. Yang and M. S. Ridout, “The Detection and Estimation of Verticillium dahliae in Naturally Infested Soil,” Plant Pathology, Vol. 42, 1993, pp. 238-250. doi:10.1111/j.1365-3059.1993.tb01496.x

[10]   Z. K. Atallah, J. Bae, S. H. Jansky, D. I. Rouse and W. R. Stevenson, “Multiplex Real-Time Quantitative PCR to Detect and Quantify Verticillium dahliae Colonization in Potato Lines that Differ in Response to Verticillium Wilt,” Phytopathology, Vol. 97, No. 7, 2007, pp. 865-872. doi:10.1094/PHYTO-97-7-0865

[11]   J. Peters, “Detecting Verticillium dahliae in Olive Platnation Soils,” Proceedings of the 11th International Verticillium Symposium, Gottingen, 5-8 May 2013, p. 70.

[12]   K. V. Subbarao and Z. Kabir, “Management of Soilborne Diseases in Strawberry Using Vegetable Rotations,” Plant Disease, Vol. 91, No. 8, 2007, pp. 964-972. doi:10.1094/PDIS-91-8-0964

[13]   J. Hiemstra, B. van der Sluis, P. van Dalfsen, A. Smits, J. Visser and G. Korthals, “Control of Verticillium in Tree Nurseries through Biological Soil Infestation,” Proceedings of the 11th International Verticillium Symposium, Gottingen, 5-8 May 2013, p. 62.

[14]   T. A. Wheeler, J. P. Bordovsky, J. W. Keeling, B. G. Mullinix and J. E. Woodward, “Effects of Crop Rotation, Cultivar, and Irrigation and Nitrogen Rate on Verticillium Wilt in Cotton,” Plant Disease, Vol. 96, No. 7, 2012, pp. 985-989. doi:10.1094/PDIS-02-11-0111-RE

[15]   A. Dressler, P. Scheewe, P. Lentzsch and K. Olbricht, “Evaluation of Strawberry Cultivars for Resistance to Verticillium dahliae Kleb,” Proceedings of the 14th Ecofruit International Conference on Organic Fruit Growing, Hohenheim, 22-24 February 2010, pp. 350-352.

[16]   J. Taylor and L. A. Harrier, “A Comparison of Development and Mineral Nutrition of Micropropagated Fragaria × Ananassa cv. Elvira (Strawberry) When Colonized by Nine Species of Arbuscular Mycorrhizal Fungi,” Applied Soil Ecology, Vol. 18, No. 3, 2001, pp. 205-215. doi:10.1016/S0929-1393(01)00164-0

[17]   J. Debode, K. De Maeyer, M. Perneel, J. Pannecoucque, G. De Backer and M. Hofte, “Biosurfactants Are Involved in the Biological Control of Verticillium microsclerotia by Pseudomonas spp.,” Journal of Applied Microbiology, Vol. 103, 2007, pp. 1184-1196. doi:10.1111/j.1365-2672.2007.03348.x

[18]   G. Berg, K. Opelt, C. Zachow, J. Lottmann, M. Gotz, R. Costa and K. Smalla, “The Rhizosphere Effect on Bacteria Antagonistic towards the Pathogenic Fungus Verticillium Differs Depending on Plant Species and Site,” FEMS Microbiology Ecology, Vol. 56, No. 2, 2006, pp. 250-261. doi:10.1111/j.1574-6941.2005.00025.x

[19]   G. Berg, C. Zachow, J. Lottmann, M. Gotz, R. Costa and K. Smalla, “Impact of Plant Species and Site on Rhizosphere-Associated Fungi Antagonistic to Verticillium dahliae Kleb,” Applied and Environmental Microbiology, Vol. 71, No. 8, 2005, pp. 4203-4213. doi:10.1128/AEM.71.8.4203-4213.2005

[20]   L. Tyvaert, S. C. Franca, J. Debode and M. Hofte, “Interaction of the Wilt Pathogen Verticillium longisporum and the Biological Control Agent Verticillium Vt305 in Cauliflower Plants,” Proceedings of the 11th International Verticillium Symposium, Gottingen, 5-8 May 2013, p. 79.

[21]   R. G. Bhat and K. V. Subbarao, “Host Range Specificity in Verticillium dahliae,” Phytopathology, Vol. 89, No. 12, 1999, pp. 1218-1225. doi:10.1094/PHYTO.1999.89.12.1218

[22]   T. R. Gordon, D. V. Shaw and K. D. Larson, “Comparative Response of Strawberries to Conidial Root-Dip Inoculations and Infection by Soilborne Microsclerotia of Verticillium dahliae Kleb,” HortScience, Vol 40, No. 5, 2005, pp. 1398-1400.

[23]   P. Schubert, J. Golldack, H. Schwarzel and P. Lentzsch, “Pathogenicity in Verticillium on Strawberry Plants,” Proceedings of the 13th Ecofruit International Conference on Cultivation Technique and Phytopathological Problems in Organic Fruit-Growing, Weinsberg, 18-20 February 2008, pp. 138-143.

[24]   P. Schubert, J. Golldack, H. Schwarzel and P. Lentzsch, “Temperature Dependent Pathogenicity of Verticillium dahliae Kleb. Populations in Strawberry Plants of the Cultivar ‘Elsanta’,” Acta Horticulturae ISHS, Vol. 842, No. 29, 2009, pp. 203-206.

[25]   P. Schubert, J. Golldack, H. Schwarzel and P. Lentzsch, “Influence of Soil Temperature to the Pathosystem Strawberry-Verticillium,” Acta Horticulturae ISHS, Vol. 838, No. 23, 2009, pp. 139-144.

[26]   P. Lentzsch, J. Golldack, H. Schwarzel and P. Schubert, “Composition and Method for the Prevention of Plant Damage Caused by Verticillium,” Patent No. WO/2007/ 051654, 2007.

[27]   P. Interbitzin, R. M. Bostock, R. M. Davis, T. Usami, H. W. Platt and K. V. Subbarao, “Phylogenetics and Taxonomy of the Fungal Vascular Wilt Pathogen Verticillium, with the Description of Five New Species,” PLoS ONE, Vol. 6, No. 12, 2011, e28341. doi:10.1371/journal.pone.0028341

[28]   T. R. Gordon, S. C. Kirkpatrick, J. Hansen and D. V. Shaw, “Response of Strawberry Genotypes to Inoculation with Isolates of Verticillium dahliae Differing in Host Origin,” Plant Pathology, Vol. 55, No. 6, 2006, pp. 766-769. doi:10.1111/j.1365-3059.2006.01459.x

[29]   T. Jecz and M. Korbin, “Inoculation of Micropropagated Plants with Wounded Roots as a Tool to Precisely Distinguish Strawberry Genotypes Tolerant and Susceptible to Verticillium Wilt Disease,” Phytopathologia, Vol. 58, No. 58, 2010, pp. 33-42.

[30]   S. Klose, V. Acosta-Martínez and H. A. Ajwa, “Microbial Community Composition and Enzyme Activities in a Sandy Loam Soil after Fumigation with Methyl Bromide or Alternative Biocides,” Soil Biology and Biochemistry, Vol. 38, No. 6, 2006, pp. 1243-1254. doi:10.1016/j.soilbio.2005.09.025

[31]   S. Klose and H. A. Ajwa, “Enzyme Activities in Agricultural Soils Fumigated with Methyl Bromide Alternatives,” Soil Biology and Biochemistry, Vol. 36, No. 10, 2004, pp. 1625-1635. doi:10.1016/j.soilbio.2004.07.009

[32]   C. Carroll, C. A. Carter and K. V. Subbarao, “California Lettuce Industry Threatened by Imported Pathogen,” ARE Update, University of California Giannini Foundation of Agricultural Economics, Vol. 14, No. 4, 2012, pp. 9-11.

[33]   C. C. Thanassoulopoulos, “Spread of Verticillium Wilt by Nursery Plants in Olive Groves in the Halkidiki Area (Greece),” EPPO Bulletin, Vol. 23, No. 3, 1993, pp. 517-520. doi:10.1111/j.1365-2338.1993.tb01363.x