ABSTRACT To examine the sex differences in the crowing behavior of the Japanese quail, we investigated the effects of sex steroids on calling behaviors using female birds, and the data were compared with those obtained in our previous study in male birds which was performed using the same experimental procedures as those in the present study. We injected the female quails daily from 11 to 41 days after hatching with testosterone propionate (TP), 5α-dihydrotestosterone (DHT; a non-aromatizable androgen), estradiol benzoate (EB) or vehicle, and examined their calling behaviors in both sexual and non-sexual contexts. In a non-sexual context of the birds being isolated in a recording chamber, androgens, either TP or DHT, induced crowing in place of distress calling while EB simply inhibited distress calling. These effects of sex steroids on the calling behaviors were almost identical to those in the male quails. In a sexual context of the birds being left undisturbed in their home cages, crowing was induced by chronic treatment with TP, but not either DHT or EB, suggesting that both estrogenic and androgenic actions are required to induce the sexually motivated crowing. Although these results were basically the same as those in the male quails, the crowing in the female quails occurred much less frequently compared to that of the male quails. These data suggest that in the Japanese quail, crowing behavior, when it is restricted to sexually motivated one, is quantitatively different between male and female in the responsiveness to sex steroids.
Cite this paper
A. Chiba and M. Fujiwara, "Roles of Sex Steroids in the Crowings with Sexual and Non-Sexual Motivations in Female Japanese Quail, Coturnix Japonica," Journal of Behavioral and Brain Science, Vol. 3 No. 3, 2013, pp. 289-295. doi: 10.4236/jbbs.2013.33028.
 J. Barthazart, M. Schumacher and M. A. Ottinger, “Sexual Differences in the Japanese Quail: Behavior, Morphology and Intracellular Metabolism of Testosterone,” General and Comparative Endocrinology, Vol. 51, No. 2, 1983, pp. 191-207. doi:10.1016/0016-6480(83)90072-2
 M. Schumacher and J. Balthazart, “The Effects of Testosterone and Its Metabolites on Sexual Behavior and Morphology in Male and Female Japanese Quail,” Physiology and Behavior, Vol. 30, No. 3, 1983, pp. 335-339.
 E. K. Adkins, “Effects of Diverse Androgens on the Sexual Behavior and Morphology of Castrated Male Quail,” Hormones and Behavior, Vol. 8, No. 2, 1977, pp. 201207. doi:10.1016/0018-506X(77)90037-X
 E. K. Adkins, J. J. Boop, D. L. Koutnik, J. B. Morris and E. E. Pniewski, “Further Evidence That Androgen Aromatization Is Essential for the Activation of Copulation in Male Quail,” Physiology and Behavior, Vol. 24, No. 3, 1980, pp. 441-446. doi:10.1016/0031-9384(80)90233-4
 E. K. Adkins and E. E. Pniewski, “Control of Reproductive Behavior by Sex Steroids in Male Quail,” Journal of Comparative, Physiological Psychology, Vol. 92, No. 6, 1978, pp. 1169-1178. doi:10.1037/h0077523
 J. Balthazart, M. Schumacher and G. Malacarne, “Relative Potencies of Testosterone and 5α-Dihydrotestosterone on Crowing and Cloacal Gland Growth in the Japanese Quail (Coturnix coturnix japonica),” Journal of Endocrinology, Vol. 100, No. 1, 1984, pp. 19-23.
 P. Deviche and M. Schumacher, “Behavioral and Morphological Dose-Responses to Testosterone and to 5α-Dihydrotestosterone in the Castrated Japanese Quail,” Behavioral Processes, Vol. 7, No. 2, 1982, pp. 107-121.
 Y. Yazaki, T. Matsushima and K. Aoki, “Testosterone Modulates Calling Behavior in Japanese Quail Chicks,” Zoological Science, Vol.14, No. 2, 1997, pp. 219-225.
 A. Chiba and N. Hosokawa, “Effects of Androgens and Estrogens on Crowings and Distress Callings in Male Japanese Quail, Coturnix japonica,” Hormones and Behavior, Vol. 49, No. 1, 2006, pp. 4-14.
 N. Hosokawa and A. Chiba, “Embryonic and Posthatching Treatments with Sex Steroids Demasculinize the Motivational Aspects of Crowing Behavior in Male Japanese Quail,” Hormones and Behavior, Vol. 55, No. 1, 2009, pp. 139-148. doi:10.1016/j.yhbeh.2008.09.005
 J. Balthazart and M. Schumacher, “Estradiol Contributes to the Postnatal Demasculinization of Female Japanese Quail (Coturnix coturnix japonica),” Hormones and Behavior, Vol. 18, No. 3, 1984, pp. 287-297.
 M. Schumacher and J. Balthazart, “The Postnatal Demasculinization of Sexual Behavior in the Japanese Quail (Coturnix coturnix japonica),” Hormones and Behavior, Vol. 18, No. 3, 1984, pp. 298-312.
 B. D. Sachs, “Photoperiodic Control of the Cloacal Gland of the Japanese Quail,” Science, Vol. 157, No. 3785, 1967, pp. 201-203. doi:10.1126/science.157.3785.201
 D. T. Davies, R. Massa and R. James, “Role of Testosterone and of Its Metabolites in Regulating Gonadotrophin Secretion in the Japanese Quail,” Journal of Endocrinology, Vol. 84, No. 1, 1980, pp. 211-222.
 J. Balthazart, M. Schumacher and G. Malacarne, “Interaction of Androgens and Estrogens in the Control of Sexual Behavior in Male Japanese Quail,” Physiology and Behavior, Vol. 35, No. 2, 1985, pp. 157-166.
 J. Balthazart and C. Surlemont, “Androgen and Estrogen Action in the Preoptic Area and Activation of Copulatory Behavior in Quail,” Physiology and Behavior, Vol. 48, No. 5, 1990, pp. 599-609.
 J. Balthazart, P. Absil, M. Gérard, D. Appeltants and G. F. Ball, “Appetitive and Consummatory Male Sexual Behavior in Japanese Quail Are Differentially Regulated by Subregions of the Preoptic Medial Nucleus,” Journal of Neuroscience, Vol. 18, No. 16, 1998, pp. 6512-6527.
 G. F. Ball and J. Balthazart, “Japanese Quail as a Model System for Studying the Neuroendocrine Control of Reproductive and Social Behaviors,” ILAR Journal, Vol. 51, No. 5, 2010, pp. 310-325. doi:10.1093/ilar.51.4.310
 G. C. Panzica, C. Viglietti-Panzica and J. Nalthazart, “The Sexually Dimorphic Medial Preoptic Nucleus of Quail: A Key Brain Area Mediating Steroid Action on Male Sexual Behavior,” Frontiers in Neuroendocrinology, Vol. 17, No. 1, 1996, pp. 51-125.