Institute of Problems of Chemical Physics, Russian Academy of Sciences, Chernogolovka, Russia.
Iron tetranitrosyl complex bearing the thiosulfate ligand (TNIC) is an efficient nitrogen monoxide donor (NO). He shows antitumor properties and may be used as an original drug for the therapy of acute coronary syndrome. In this work, the reaction of the TNIC with adenosine triphosphoric acid (ATP) was studied. Formation of the products for the reaction of ATP with TNIC was shown by electronic microscopy. The kinetics of the reaction was controlled by spectrofluorometric method, and the complexation constant was measured. The mechanism of interaction of ATP with TNIC was proposed, and the relevant kinetic model satisfactorily described the experimental data, which permitted to calculate the rate constants for these process stages. NMR, IR, and M?ssbauer studies were used for determination of the reaction product structure. NMR study showed TNIC interaction only with adenine part of ATP. The method of IR spectroscopy identified both the absence NO in the reaction products and the occurrence of new Fe-S and Fe-N bonds. M?ssbauer study showed that iron in the reaction products was presented by two forms: Fe(II) and Fe(III). Thus, the structures for the [ATP-Fe2+S] and [ATP-Fe3+S] complexes were proposed.
Saratovskikh, E. , Psikha, B. and Sanina, N. (2013) The reaction of the iron thiosulfate-nitrosyl complex with adenosine triphosphoric acid. Natural Science, 5, 800-810. doi: 10.4236/ns.2013.57097.
[1] Lehninger, A. (1983) Principles of biochemistry. W. H. Freeman, London. [2] Obrezan, A.G. and Raimuev, K.V. (2009) Chronic cardiac deficiency: The state of the problem at the modent stage. Vestn. St. Petersburg Post-Diploma Education Medical Academy, 1, 5. (in Russian) [3] Bokeriya, L.A. and Chicherin, I.N. (2007) Nature and clinic significance of “new ischemic syndromes”. A.N. Bakulev NTsSSKh, Russian Academy of Medical Sciences, Moscow. (in Russian) [4] Belovol, A.N. and Knyazrkova, I.I. (2009) Prospects of pharmacological correction of hypoxia in cardiology and neurology. Liki Ukraini plyus, 3, 4. (in Russian) [5] Erickson, R.R., Yu-Drend, P. and Holtzman, J.L. (1982) The kinetics ethylmorpfine N-demethylase in isolated hepatocydes: The effect of drug transport and oxygen concentration. Journal of Pharmacology and Experimental Therapeutics, 220, 35. [6] Weiss, J.M., Lamp, S.T. and Shine, K.I. (1989) Celular K+ loss and union efflux during myocardial ischemia and metabolic inhibition. American Journal of Physiology, 256, 1165. [7] Todorov, I.N. (2007) Mitochondria: Oxidative stress and mitochondrial DNA mutations in the development of pa thologies, the process of aging and apoptosis. Rossiiskii Khimicheskii Zhurnal, 51, 93. [8] Grazhdan, K.V. (2009) Complexation of Fe(III) with nikotinamidom in aqueous solutions of ethanol and dime thylsulphoxide. Ph.D. Thesis, State Medicine Academy, Ivanovo. (in Russian). [9] Dobrokhotova, O.V., Tatryanenko, L.V., Kotelrnikov, A.I., Saratovskikh, E.A., Rudneva, T.N., Sanina, N.A. and Al doshin, S.M. (2009) The impact of nitroxide iron-sulphur complexes on the activity of hydrolytic enzymes. Khimiko Farmatsevticheskii Zhurnal, 43, 45. [10] Sanina, N.A. (2010) The study of cardioprotective properties nitrosyl iron complexes donor-NO, with the use of models of ischemic and reperfusion damage of myocardium in vitro and in vivo. 18th International Conference on Novel Informational Technologies in Medicine, Biology, Pharmacology, and Ecology, Yalta-Gurzuf, 31 May 9 June 2010, 348. [11] Sanina, N.A., Rudneva, T.N., Sulimenkov, I.V., Kono valova, N.P., Sashenkova, T.E. and Aldoshin, S.M. (2009) Antitumor activity of iron nitrosyl complexes: New donors of nitrogen monoxide. Rossiiskii Khimicheskii Zhur nal, 53, 164. [12] Sanina, N.A., Serebryakova, L.I., Shul’zhenko, V.S., Pisarenko, O.I., Rudneva, T.N. and Aldoshin, S.M. (2011) Application of the binuclear anionic sulfur nitrosyl iron complex as a vasodilatory drug. RF Patent No. 2437667. [13] Sanina, N.A. (2011) A new class of donors carbon monoxide nitrogen: Structure and properties of nitrosyl iron complexes with functional sulfur ligands. Ph.D. Thesis, Institute of Problems of Chemical Physics Russian Academy of Sciences, Chernogolovka. (in Russian) [14] Saratovskikh, E.A., Kondratreva, T.A., Psikha, B.L., Gvozdev, R.I. and Kartsev, V.G. (1988) Complex-formation of some pesticides with adenosine triphosphoric acide. Akademiia nauk SSSR. Izvestiia. Seriia khimiche skaia, 37, 2501. [15] Sanina, N.A., Aldoshin, S.M., Rudneva, T.N., Golovina, N.I., Shilov, G.N., Shulrga, Yu. M., Martynenko, V.M. and Ovanesyan, N.S. (2005) Synthesis, structure, and solid phase transformations of the nitrosyl iron complex Na2[Fe2(S2O3)2(NO)4] 4H2O. Koordinatsionnaya Khimiya, 31, 323. [16] Dibbern, H.W., Muller, R.M. and Wirbitzki, E. (2002) UV and IR spectra of some important drugs. Publ. Editio Cantor VERLAG (ECV), Berlin. [17] Nakanishi, K. (1962) Infrared adsorption spectroscopy. Holden Day, San Francisco. [18] Nakamoto, K. (1963) Infrared spectra of inorganic and coordination compounds. Marquette University, Milwau kee. [19] Sverdlov, L.M., Kovner, M.A. and Krainov, E.P. (1970) Kolebatel’nye spektry mnogoatomnykh molekul [Vibrational Spectra of Polyatomic Molecules], Nauka, Moscow. (in Russian) [20] Burshtein E.A. (1977) Own luminescence of proteins: The nature and application. Science and Technics in Review, Ser. Biophysics, VINITI, Moscow, 7, 139. (in Russian) [21] Vasilreva, S.V., Moshkovskaya, E. Yu., Sanina, N.A., Rudneva, T.N., Kulikov, A.V. and Aldoshin, S.M. (2005) Formation of the dinitrosyl iron complex as a necessary stage in the accomplishment of genetic activity. Doklady Akademii Nauk, 402, 705. [22] Williams, V.R. (1973) Basic physical chemistry for the life. Sciences, San Francisco.