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 OJAS  Vol.3 No.3 , July 2013
Effect of heat stress on the maturation, fertilization and development rates of in vitro produced bovine embryos
Abstract: Heat stress is one of the main reasons for reproductive performance decrease in cattle, resulting in severe economic losses. The aim of this study was to evaluate the effect of heat stress during maturation, fertilization and development of in vitro produced bovine embryos. Cumulus oocyte complexes (COCs) were obtained by follicular puncture from slaughterhouse ovaries and after identification, were divided into four groups: control (CG), exposed 1 (EG1), exposed 2 (EG2), and exposed 3 (EG3). The oocytes of the group CG and CG3 were cultured at 38°C and the oocytes of group EG1 and EG2 were cultured at 40°C during the maturation period (24 hours at 5% CO2 in air). After the maturation period, oocytes of group CG, EG1, EG2, and EG3 were fecundated with frozen thawed semen. The oocytes of CG, EG2 and EG3 groups were cultured at 38°C, and the group EG1 was cultured at 40°C (18 hours at 5% CO2 in air). After that, the CG and EG2 groups were cultured in SOF at 38°C and the groups EG1 and EG3 at 40°C during embryonic development. The embryos were evaluated for cleavage, morula and blastocyst rates by optical microscopy. In control (CG) and EG3 groups, the oocytes showed uniform expansion of cumulus cells, classified as moderate to high, with brown color and uniform appearance of the ooplasm. In the oocytes exposed to 40°C (EG1 and EG2) we observed a decrease in the expansion of cumulus cells, and the same showed rounded appearance and retraction of the ooplasm with dark coloration. The control group (CG) had 68.23% ± 2% of cleavage, 50.16% ± 2% morulas, and 43.28% ± 1% blastocysts. Whereas the EG2 had 31.46% ± 2% cleavage, 35.64% ± 2% morula, and no blastocysts development. The EG3 had 3.7% ± 2% cleavage, and no embryo production. These data suggest that in all stages of exposure to heat stress, the embryos and the gametes are susceptible, leading to a decrease in embryonic development.
Cite this paper: Alves, M. , Gonçalves, R. , Pavão, D. , Palazzi, E. , Souza, F. , Queiróz, R. , Angelo, M. and Achilles, M. (2013) Effect of heat stress on the maturation, fertilization and development rates of in vitro produced bovine embryos. Open Journal of Animal Sciences, 3, 174-178. doi: 10.4236/ojas.2013.33026.
References

[1]   [1] Pires, M.F.A., Ferreira, A.M. and Coelho, S.G. (1999) Estresse calórico em Bovinos de Leite. Caderno técnico de Veterinária e Zootecnia, 29, 23-37.

[2]   Encarnacao, R.O. (1992) Estresse e producao animal. Ph.D. Dissertation, EMBRAPA-CNPGC, Campo Grande.

[3]   Santos Junior, E.R. (2010) Efeito do estresse térmico na maturacao in vitro de oócitos caprinos e ovinos em protocolo de producao de embrioes. Ph.D. Dissertation, Universidade Federal Rural de Pernambuco, Recife.

[4]   Ferro, F.R.A., Cavalcanti Neto, C.C., Toledo Filho, M.R., Ferri, S.T.S. and Montaldo, Y.C. (2010) Efeito do estresse calórico no desempenho reprodutivo de vacas leiteiras. Revista Verde, 5, 1-25.

[5]   Hansen, P.J. and Aréchiga, C.F. (1999) Strategies for managing reproduction in the heat-stressed dairy cow. Journal of Animal Science, 77, 36-50.

[6]   Paula-Lopes, F.F. and Hansen, P.J. (2002) Heat-shock induced apoptosis in preimplantation bovine embryos is a developmentally-regulated phenomenon. Biology of Reproduction, 66, 1169-1177.

[7]   Pires, M.F.A., Ferreira, A.M. and Coelho, S.G. (1999) Estresse calórico em Bovinos de Leite. Caderno técnico de Veterinária e Zootecnia, 29, 23-37.

[8]   Vianna, F.P. (2002) Influência do estresse térmico na atividade reprodutiva de fêmeas bovinas. Ph.D. Dissertation, Universidade Estadual de Sao Paulo, Botucatu.

[9]   Roman-Ponce, H., Thatcher, W.W., Caton, D., Barron, D.H. and Wilcox, C.J. (1978) Thermal stress effects on uterine blood flow in dairy cows. Journal of Animal Science, 46, 175-180.

[10]   Edwards, J.L. and Hansen, P.J. (1996) Elevated temperature increases heat shock protein 70 synthesis in bovine two-cell embryos and compromises function of maturing oocytes. Biology of Reproduction, 55, 340-346. doi:10.1095/biolreprod55.2.341

[11]   Zeron, Y., Ocheretny, A., Kedar, O., Borochov, A., Sklan, D. and Arav, A. (2001) Seasonal changes in bovine fertility: Relation to developmental competence of oocytes, membrane properties and fatty acid composition of follicles. Reproduction, 121, 447-454. doi:10.1530/rep.0.1210447

[12]   Al-Katanani, Y.M. and Hansen, P.J. (2002) Induced thermotolerance in bovine two cells embryos and the role of heat schock protein 70 in embryonic development. Molecular Reproduction and Development, 62, 174-180. doi:10.1002/mrd.10122

[13]   Ealy, A.D., Drost, M. and Hansen, P.J. (1993) Developmental changes in embryonic resistance to adverse effects of maternal heat stress in cows. Journal of Dairy Science, 76, 2899-2905. doi:10.3168/jds.S0022-0302(93)77629-8

[14]   De Souza, P.A., Watson, A.J., Schultz, G.A. and Bilodeau-Goessels, S. (1998) Oogenetic and zygotic gene expression directing early bovine embryogenesis: A review. Molecular Reproduction and Development, 51, 112-121. doi:10.1002/(SICI)1098-2795(199809)51:1<112::AID-MRD14>3.0.CO;2-9

[15]   Saeki, K., Matsumoto, K., Kaneko, T., Hosol, Y., Kato, H. and Iritani, A. (1999) Onset of RNA syntesis in early bovine embryos detected by reverse transcripitionpolymerase chain reaction following introduction of exogenous gene into their pronuclei. Theriogenology, 51, 192. doi:10.1016/S0093-691X(99)91751-X

[16]   Goncalves, R.F., Chapman, D.A., Bertolla, R.P., Eder, I., and Killian, G.J. (2008) Pre treatment of cattle semen or oocytes with purified milk osteopontin affects in vitro fertilization and embryo development. Animal Reproduction Science, 108, 375-383. doi:10.1016/j.anireprosci.2007.09.006

[17]   Van Wagtendonk-de Leeuw, A.M., Mullaart, E., de Ross, A.P., Merton, J.S., den Daas, J.H., Kemp, B. and de Ruigh, L. (2000) Effects of different reproduction techniques: AI, MOET, or IVP, on health and welfare of bovine offspring. Theriogenolog, 53, 575-597. doi:10.1016/S0093-691X(99)00259-9

[18]   Edwards, J.L. and Hansen, P.J. (1997) Differential responses of bovine oocytes and preimplantation embryos to heat shock. Molecular Reproduction and Development, 46, 138-145. doi:10.1002/(SICI)1098-2795(199702)46:2<138::AID-MRD4>3.0.CO;2-R

[19]   Ju, J.C., Parks, J.E. and Yang, X. (1999) Thermotolerance of IVM-derived bovine oocytes and embryos after short term heat stress. Molecular Reproduction and Development, 53, 336-340. doi:10.1002/(SICI)1098-2795(199907)53:3<336::AID-MRD9>3.0.CO;2-M

[20]   Ealy, A.D., Howell, J.L., Monterroso, V.H., Arechiga, C.F. and Hansen, P.J. (1995) Developmental changes in sensitivity of bovine embryos to heat shock and use of antioxidants as thermoprotectants. Journal of Animal Science, 73, 1401-1407.

[21]   Hansen, P.J., Rivera, R.M., Paula Lopes, F.F., Al-Katanani, Y.M., Krininger III, C.E. and Chase Jr., C.C. (2001) Adverse impact of heat stress on embryo production: Causes and strategies for mitigation. Theriogenology, 51, 91-103. doi:10.1016/S0093-691X(00)00448-9

[22]   Monterroso, V.H., Drury, K.C., Baly, A.D., Edwards, J.L. and Hansen, P.J. (1995) Effect of heat shock on function of frozen/thawed bull spermatozoa. Theriogenology, 44, 947-961. doi:10.1016/0093-691X(95)00282-D

[23]   Krininger III, C.E., Block, A.J., Al-Katanani, Y.M., Rivera, R.M., Case Jr., C.C. and Hansen, P.J. (2003) Differences between Brahman and Holstein cows in response to estrus synchronization, superovulation and resistance of embryos to heat shock. Animal Reproduction Science, 78, 13-24. doi:10.1016/S0378-4320(03)00045-9

[24]   Schultz, R.M. (2002) The molecular foundations of the maternal to zygotic transition in the preimplantation embryo. Human Reproduction Update, 8, 323-331. doi:10.1093/humupd/8.4.323

[25]   Memili, E. and First, N.L. (2000) Zygotic and embryonic gene expression in cow: A review of timing and mechanisms of early gene expression as compared with other species. Zygote, 8, 87-96. doi:10.1017/S0967199400000861

[26]   Tseng, J.K., Chen, C.H., Chou, P.C., Yeh, S.P. and Ju, J.C. (2004) Influences of follicular size on parthenogenetic activation and in vitro heat shock on the cytoskeleton in cattle oocytes. Reproduction of Domestic Animals, 39, 146-153. doi:10.1111/j.1439-0531.2004.00493.x

[27]   Ju, J.C. and Tseng, J.K. (2004) Nuclear and cytoskeletal alterations of in vitro matured porcine oocytes under hyperthermia. Molecular Reproduction and Development, 68, 125-133. doi:10.1002/mrd.20054

[28]   Bényei, B. and Barros, C.W.C. (2000) Variacoes fisiológicas de parametros reprodutivos em vacas de raca Holandesa importadas da Hungria para o Nordeste brasileiro. Brazilian Journal of Veterinary Research and Animal Science, 37, 3. doi:10.1590/S1413-95962000000300008

 
 
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