AS  Vol.4 No.7 , July 2013
Soybean meal as a source of protein in formulated diets for tiger grouper, Epinephelus fuscoguttatus juvenile. Part I: Effects on growth, survival, feed utilization and body compositions

The effects of fish meal (FM) replacement with graded level of soybean meal (SBM) on growth performance, feed utilization, survival rate and body composition were investigated in juvenile tiger grouper, Epinephelus fuscoguttatus (initial body weight 13.9 ±0.65 g). Six experimental diets were formulated to contain 50% crude protein, 16% crude lipid and 365.8 kcal/100g feed with SBM replacing FM protein at 0% (SM 0), 10% (SM 0), 20% (SM 20), 30% (SM 30), 40% (SM 40) and 20% with phytase (SM 20P) replacement levels. At the end of the ten-week feeding trial, there were no significant differences detected in terms of growth performances (weight gain and specific growth rate), feed conversion ratio (FCR) and survival rates of fish fed with the control diet (SM 0), SM 20, SM 30 and SM 20P. Net protein utilization of fish fed SM 20P was higher than those fed with other diets suggesting an improved utilization of nutrients with phytase addition in the diet. Replacements of FM protein with SBM at 10% and 40% have resulted in significantly lower growth and poorer FCR than other replacement levels. Survival rates remain high (≥90%) throughout the trial. Whole-body proximate composition of the fish was significantly affected by the inclusion of SBM in the diets. It can be concluded that 20%-30% of FM protein replacement with SBM is recommended and addition of phytase in the SBM-based diet should be considered to improve nutrient utilization of tiger grouper juvenile.

Cite this paper: Shapawi, R. , Ebi, I. and Yong, A. (2013) Soybean meal as a source of protein in formulated diets for tiger grouper, Epinephelus fuscoguttatus juvenile. Part I: Effects on growth, survival, feed utilization and body compositions. Agricultural Sciences, 4, 317-323. doi: 10.4236/as.2013.47045.

[1]   Cheng, Z.J. and Hardy R.W. (2002) Effect of microbial phytase on apparent nutrient digestibility of barley, canola meal, wheat and wheat middlings, measured in vivo using rainbow trout (Oncorhynchus mykiss). Aquaculture Nutrition, 8, 271-277. doi:10.1046/j.1365-2095.2002.00219.x

[2]   Brown, P.B., Kaushik, S.J. and Peres, H. (2008) Protein dietstuffs originating from soybeans. In: Lim, C., Webster, C.D. and Lee, C., Eds., Alternative Protein Sources in Aquaculture Diets. The Haworth Press, New York, 205-223.

[3]   Silva-Carillo, Y., Hernandez, C., Hardy, R.W., Gonzalez-Rodriguez, B. and Castillo-Vargasmachuca, S. (2012) The effect of substituting fish meal with soybean meal on growth, fees efficiency, body composition and blood chemistry in juvenile spotted rose snapper Lutjanus guttatus (Steindachner, 1869). Aquaculture, 364-365, 180-185. doi:10.1016/j.aquaculture.2012.08.007

[4]   Tantikitti, C., Sangpong, W. and Chiavareesajja, S. (2005) Effects of deffated soybean protein levels on growth performance and nitrogen and phosphorus excretion in Asian seabass (Lates calcarifer). Aquaculture, 248, 41-50. doi:10.1016/j.aquaculture.2005.04.027

[5]   Lim, S.-J., Kim, S.-S., Ko, G.-Y., Song, J.-W., Oh, D.-H., Kim, J.-D., Kim, J.-U. and Lee, K.-J. (2011) Fish meal replacement by soybean meal in diets for tiger puffer, Takifugu rubripes. Aquaculture, 313, 165-170. doi:10.1016/j.aquaculture.2011.01.007

[6]   Amerio, M., Mazzola, M., Caridi, D., Crisafi, E. and Genovese, L. (1991) Soybean products in diets for sea bass. Proceedings of the IX National ASPA Congress, ASA, Roma, 3-7 June 1991.

[7]   Kikuchi, K. (1999) Use of deffated soybean meal as a substitute for fish meal in diets of Japanese flounder (Paralicthys oliveaceus). Aquaculture, 179, 3-11. doi:10.1016/S0044-8486(99)00147-7

[8]   Biswas, A.K., Kaku, H., Ji, S.C., Seoka, M. and Takii, K. (2007) Use of soybean meal and phytase for partial replacement of fishmeal in the diet of red sea bream, Pagrus major. Aquaculture, 267, 284-291. doi:10.1016/j.aquaculture.2007.01.014

[9]   Association of Official Analytical Chemists International (AOAC International) (1990) Official methods of analysis of AOAC international. 16th Edition, AOAC International, Arlington.

[10]   Bonaldo, A., Roem, A.J., Pecchini, A., Grilli, E. and Gatta, P.P. (2006) Influence of dietary soybean meal levels on growth, feed utilization and gut histology of Egyptian sole (Solea aegyptiaca) juveniles. Aquaculture, 261, 580-586. doi:10.1016/j.aquaculture.2006.08.013

[11]   Kader, M.A., Bulbul, M., Kashio, S., Ishikawa, M., Yokohama, S., Nguyen, B.T. and Komilus, C.F. (2012) Effect of complete replacement of fishmeal by dehulled soybean meal with crude attractant supplementation in diets for red sea bream, Pagrus major. Aquaculture, 350-353, 109-116. doi:10.1016/j.aquaculture.2012.04.009

[12]   Ukawa, M., Takii, K., Nakamura, M. and Kumai, H. (1994) Utilization of soybean meal for red sea bream diet. Suisanzoshoku, 42, 335-338.

[13]   Pongmaneerat, J. and Watanabe, T. (1993) Nutritional evaluation of soybean meal for rainbow trout and carp. Nippon Suisan Gakkaishi, 59, 157-163. doi:10.2331/suisan.59.157

[14]   Luo, Z., Liu, Y.J., Mai, K.S., Tian, L.X., Liu, D.H. and Tan, X.Y. (2004) Optimal dietary protein requirement of grouper Epinephelus coioides juveniles fed isoenergetic diets in floating net cages. Aquaculture Nutrition, 10, 247-252. doi:10.1111/j.1365-2095.2004.00296.x

[15]   Refstie, S., Korsoen, O.J., Storebakken, T., Baeverfjord, G., Lein, I. and Roem, A.J. (2000) Differing nutritional responses to dietary soybean meal in rainbow trout (Oncorhynchus mykiss) and Atlantic salmon (Salmo salar). Aquaculture, 190, 49-63. doi:10.1016/S0044-8486(00)00382-3

[16]   Shapawi, R., Ng, W.K. and Mustafa, S. (2007) Replacement of fish meal poultry by-product meal in diets formulated for the humpback grouper, Cromileptes altivelis. Aquaculture, 237, 118-126. doi:10.1016/j.aquaculture.2007.09.014

[17]   Tidwell, J.H., Coyle, S.D., Bright, L.A. and Yasharian, D. (2005) Evaluation of plant and animal source proteins for replacement of fish meal in practical diets for the largemouth bass Micropterus salmoides. Journal of the World Aquaculture Society, 36, 454-462. doi:10.1111/j.1749-7345.2005.tb00393.x

[18]   Liu, B.L., Rafing, A. and Tzeng, Y.M. (1998) The induction and characterization of phytase and beyond. Enzyme Microbe Technology, 22, 415-424. doi:10.1016/S0141-0229(97)00210-X

[19]   Sugiura, H., Gabaudan, J., Dong, F.M. and Hardy, R.W. (2001) Dietary microbial phytase supplementation and the utilization of phosphorus, trace minerals, and protein by rainbow trout Oncorhynchus mykiss (Walbum) fed soybean meal-based diets. Aquaculture Research, 32, 583-592. doi:10.1046/j.1365-2109.2001.00581.x

[20]   Sørensen, M., Stjepanovic, N., Romarheim, O.H., Krekling, T. and Storebakken, T. (2009) Soybean meal improves the physical quality of extruded fish feed. Animal Feed Science and Technology, 149, 149-161. doi:10.1016/j.anifeedsci.2008.05.010

[21]   Davis, D.A., Jirsa, D. and Arnold, C.R. (1995) Evaluation of soybean proteins as replacements for menhaden fish meal in practical diets for red drum (Sciaenops ocellatus). Journal of the World Aquaculture Society, 26, 48-58. doi:10.1111/j.1749-7345.1995.tb00208.x

[22]   Meilahn, C.W., Davis, D.A. and Arnold, C.R. (1996) Effects of commercial fish meal analog and menhaden fish meal on growth of red drum fed isonitrogenous diets. Progressive Fish-Culturist, 58, 111-116. doi:10.1577/1548-8640(1996)058<0111:EOCFMA>2.3.CO;2

[23]   Lim, S.R., Choi, S.M., Wang, W.J., Kim, K.W., Shin, I.S., Min, T.S. and Bai, S.C. (2004) Effects of dehulled soybean meal as fishmeal replacer in diets for fingerling and growing Korean rockfish Sebastes schlegi. Aquaculture, 231, 457-468. doi:10.1016/j.aquaculture.2003.09.008

[24]   Chen, W., Ai, Q., Mai, K., Xu, W., Liufu, Z., Zhang, W. and Cai, Y. (2011) Effects of dietary soybean saponins on feed intake, growth performance, digestibility and intestinal structure in juvenile Japanese flounder (Paralichthys olivaceus). Aquaculture, 318, 95-100. doi:10.1016/j.aquaculture.2011.04.050

[25]   Francis, G., Karem, Z., Makkar H.P.S. and Becker K. (2002) The biological action of saponins in animal systems—A review. Britain Journal of Nutrition, 88, 587-605. doi:10.1079/BJN2002725

[26]   Bowyer, J.N., Qin, J.G., Smullen, R.P., Adams, L.R., Thomson, M.J.S. and Stone, D.A.J. (2013) Aquaculture, 384-387, 35-45.

[27]   Hernàndez, M.D., Martínez, F.J., Jover, M. and García, G.B. (2007) Effects of partial replacement of fish meal by soybean meal in sharpsnout seabream (Diplodus puntazzo) diet. Aquaculture, 263,159-167. doi:10.1016/j.aquaculture.2006.07.040

[28]   Deng, J., Mai, K, Ai, Q., Zhang, W., Wang, X., Xu, W. and Liufu, Z. (2006) Effects of replacing fish meal with soy protein concentrate on feed intake and growth of juvenile Japanese flounder, Paralichthys olivaceus. Aquaculture, 258, 503-513. doi:10.1016/j.aquaculture.2006.04.004

[29]   Luo, Z., Liu, Y.-J., Mai, K.-S., Tian, L.-X., Tan, X.-Y., Yang, H.-J., Liang, G.-Y. and Liu, D.-H. (2006) Quantitative lysine requirement of juvenile grouper Epinephelus coiedes. Aquaculture Nutrition, 12, 165-172. doi:10.1111/j.1365-2095.2006.00392.x

[30]   Luo, Z., Liu, Y.-J., Mai, K.-S., Tian, L.-X., Yang, H.-J. and Liu, D. (2005) Dietary l-methionine requirement of juvenile grouper Epinephelus coiedes at a constant dietary cystine level. Aquaculture, 249, 409-418. doi:10.1016/j.aquaculture.2005.04.030

[31]   Davis, D.A., Miller, C.L. and Phelps, R.P. (2005) Replacement of fish meal with soybean meal in the production diets of juvenile red snapper, Lutjanus campecthanus. Journal of the World Aquaculture Society, 36, 114-119. doi:10.1111/j.1749-7345.2005.tb00137.x

[32]   Antolovic, N., Kozul, V., Antolovic, M. and Bolotin, J. (2012) Effects of partial replacement of fishmeal by soybean meal on growth of juvenile saddled bream (Sparidae). Turkish Journal of Fisheries and Aquatic Sciences, 12, 247-252.

[33]   Elangovan, A. and Shim, K.F. (2000) The influence of replacing fish meal partially in the diet with soybean meal on growth and body composition of juvenile tin foil barb (Barbodes altus). Aquaculture, 189, 133-144. doi:10.1016/S0044-8486(00)00365-3

[34]   Olli, J.J., Krogdahl, A. and Vabeno, A. (1995) Dehulled solvent extracted soybean meal as a protein source in diets for Atlantic salmon, Salmo salar L. Aquaculture Research, 26,167-174. doi:10.1111/j.1365-2109.1995.tb00899.x

[35]   Mohsen, A.A. and Lovell, R.T. (1990) Partial substitution of soybean meal with animal protein sources in diets for channel catfish. Aquaculture, 90, 303-311. doi:10.1016/0044-8486(90)90254-K

[36]   Satoh, S., Takeuchi, T. and Watanabe, T. (1984). Effects of starvation and environmental temperature on proximate and fatty acid composition of Tilapia nilotica. Bulletin of Japan Society for the Science of Fish, 50, 79-84. doi:10.2331/suisan.50.79

[37]   Mahdi, A.A. (2006) Effects of starvation on the proximate chemical composition of the juveniles bunnyi, Barbus sharpeyi. Iraqi Journal of Aquaculture, 1, 11-16.